BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	549,751	A→T	14.8%	V141V (GTA→GTT)	tufB →	elongation factor Tu‑B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	549,751	0	A	T	14.8%	443.8 / 54.7	155	V141V (GTA→GTT)	tufB	elongation factor Tu‑B
Reads supporting (aligned to +/- strand): ref base A (63/69); new base T (10/13); total (73/82)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 158 total aligned reads displayed.

ATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACC  >  NC_002947/549602‑549898

aTGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTa                                                                                                                                                     <  2:3813548/150‑1 (MQ=37)

aTGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTa                                                                                                                                                     <  2:3813335/150‑1 (MQ=37)

   aTCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgac                                                                                                                                                  <  2:2829894/150‑1 (MQ=37)

       ccGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGacgacg                                                                                                                                              >  1:4526336/1‑150 (MQ=37)

       ccGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGacgacg                                                                                                                                              <  2:2735014/150‑1 (MQ=37)

            gctgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGaa                                                                                                                                         >  1:5030513/1‑149 (MQ=2)

            gctgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGaa                                                                                                                                         <  2:3102439/150‑2 (MQ=2)

             ctgcACAGATGGACGGCGCGATCCTGGTTGTTTCGTCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTAAGGTTTGCGTTACTGCGATCTTCGTGTTCCTGTACAAGTGCGATCAGGTTGACGATGCTGaac                                                                                                                                        <  1:3455175/150‑3 (MQ=2)

             ctgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGaac                                                                                                                                        <  1:2530517/150‑3 (MQ=2)

              tgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAact                                                                                                                                       >  2:499446/1‑150 (MQ=2)

              tgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAact                                                                                                                                       >  1:2949122/1‑150 (MQ=2)

              tgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAact                                                                                                                                       >  2:1037575/1‑150 (MQ=2)

                cccAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgc                                                                                                                                     >  1:177188/1‑150 (MQ=37)

                 acAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttct                                                                                                                                    >  2:365042/2‑150 (MQ=2)

                  cAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGctgctg                                                                                                                                   >  1:992513/1‑150 (MQ=37)

                        gACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTg                                                                                                                             <  2:321270/150‑1 (MQ=39)

                         aCGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACtcg                                                                                                                            >  2:2488760/1‑148 (MQ=255)

                             cgcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCga                                                                                                                        <  2:3270179/150‑1 (MQ=39)

                              gcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgag                                                                                                                       >  1:764957/1‑150 (MQ=39)

                                  tCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATgg                                                                                                                   >  1:2249327/1‑150 (MQ=39)

                                  cccTGGTTTGCGCGGCCGCCGATGGGCCGATGCCCCAAACGCGAGCGCCCATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGACTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGTCCTGGTCGTGATgg                                                                                                                   <  2:4574220/149‑1 (MQ=11)

                                    cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                 <  1:2138188/150‑1 (MQ=39)

                                    cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                 <  1:4926937/150‑1 (MQ=39)

                                    cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                 <  1:166901/150‑1 (MQ=39)

                                    cTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                 <  2:4902973/150‑1 (MQ=39)

                                     tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                                                                                                                >  1:2286608/1‑150 (MQ=39)

                                          tGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgc                                                                                                           <  1:1357214/150‑1 (MQ=39)

                                          tGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgc                                                                                                           <  1:1357179/150‑1 (MQ=39)

                                          gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTcgc                                                                                                           <  2:2623243/147‑1 (MQ=255)

                                            cTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGa                                                                                                         <  1:1013498/150‑1 (MQ=39)

                                            cTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGa                                                                                                         <  1:4072616/150‑1 (MQ=39)

                                             tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc                                                                                                        >  2:4580866/1‑150 (MQ=39)

                                             tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc                                                                                                        >  1:3629479/1‑150 (MQ=39)

                                             tCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGaa                                                                                                        <  1:3032475/150‑2 (MQ=255)

                                               ggCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCTGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACct                                                                                                      <  1:4393599/150‑1 (MQ=255)

                                                gccgccGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCTGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctg                                                                                                     <  2:627065/150‑1 (MQ=255)

                                                 ccgccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgc                                                                                                    <  2:3266669/150‑1 (MQ=39)

                                                  cgccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgct                                                                                                   >  2:4136737/1‑150 (MQ=39)

                                                     cgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTc                                                                                                <  1:3641710/150‑1 (MQ=39)

                                                     cgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTc                                                                                                <  1:4580866/150‑1 (MQ=39)

                                                      gacggcccGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTcg                                                                                               <  1:1194474/144‑2 (MQ=255)

                                                       aTGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCa                                                                                              >  2:3608343/1‑150 (MQ=39)

                                                        tGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCAc                                                                                             <  2:2213400/150‑1 (MQ=39)

                                                        tGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCAc                                                                                             >  2:4660309/1‑150 (MQ=39)

                                                              gATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGa                                                                                       >  1:3431538/1‑150 (MQ=39)

                                                                  ccacagaCCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTc                                                                                   <  2:2154964/144‑1 (MQ=255)

                                                                   cGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTcc                                                                                  >  2:4973741/1‑150 (MQ=38)

                                                                    gCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTccc                                                                                 >  1:4247954/1‑150 (MQ=38)

                                                                    acagaCCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTccc                                                                                 >  2:2582768/5‑150 (MQ=255)

                                                                     cAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCa                                                                                <  2:2926706/150‑1 (MQ=37)

                                                                          ccGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGcga                                                                           >  2:2343177/1‑150 (MQ=37)

                                                                           cGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGcgac                                                                          <  1:3889972/150‑1 (MQ=37)

                                                                                gCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGacac                                                                     >  2:1552020/1‑150 (MQ=37)

                                                                                  acaTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTc                                                                   <  2:2814316/150‑1 (MQ=37)

                                                                                    aTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCg                                                                 >  2:4380830/1‑150 (MQ=37)

                                                                                    aTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCg                                                                 >  2:1204279/1‑150 (MQ=37)

                                                                                     tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGa                                                                <  1:4116283/150‑1 (MQ=37)

                                                                                      ccTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGat                                                               >  1:143384/1‑150 (MQ=37)

                                                                                         gctgTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGatcat                                                            <  2:1317540/150‑1 (MQ=35)

                                                                                            cgctcGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGCGACGAAGTTCCAatcatcaa                                                         <  2:386739/146‑2 (MQ=2)

                                                                                                cGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGt                                                     >  1:792941/1‑150 (MQ=34)

                                                                                                 gTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGtt                                                    >  2:4350487/1‑150 (MQ=34)

                                                                                                 gTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGtt                                                    >  2:1393129/1‑150 (MQ=34)

                                                                                                 gTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGtt                                                    >  1:1471215/1‑150 (MQ=34)

                                                                                                  tCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTc                                                   <  2:4211650/150‑1 (MQ=34)

                                                                                                  tCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTc                                                   >  2:3166029/1‑150 (MQ=34)

                                                                                                     ggTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGc                                                <  1:4655137/150‑1 (MQ=34)

                                                                                                     ggTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGc                                                <  1:4273022/150‑1 (MQ=34)

                                                                                                      gTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCt                                               >  1:1606371/1‑150 (MQ=34)

                                                                                                       tAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTc                                              >  1:2212488/1‑150 (MQ=34)

                                                                                                          gCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTa                                           <  1:1099791/150‑1 (MQ=34)

                                                                                                          gCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTa                                           >  2:1147279/1‑150 (MQ=34)

                                                                                                          gCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTa                                           <  1:1234137/150‑1 (MQ=34)

                                                                                                               ccGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGcc                                      <  2:1731947/150‑1 (MQ=35)

                                                                                                                 gTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGAGTTCCCAGGCGACGACACTCCGATCATCATCGGTTGCGCTCGTATGGCCCt                                    >  2:4013063/1‑150 (MQ=255)

                                                                                                                 gTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCt                                    <  1:251084/150‑1 (MQ=37)

                                                                                                                 gTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCt                                    >  1:3206915/1‑150 (MQ=37)

                                                                                                                     aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                <  1:3921280/150‑1 (MQ=37)

                                                                                                                     aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                <  1:667505/150‑1 (MQ=37)

                                                                                                                      tCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAg                               <  1:4107808/150‑1 (MQ=37)

                                                                                                                        gTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGc                             >  1:4822298/1‑150 (MQ=37)

                                                                                                                         tGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCa                            >  1:3711028/1‑150 (MQ=37)

                                                                                                                           gTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCaaa                          <  1:4733469/150‑1 (MQ=37)

                                                                                                                           gTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCaaa                          <  2:1703632/150‑1 (MQ=37)

                                                                                                                              ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgac                       >  1:1326752/1‑150 (MQ=37)

                                                                                                                              ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgac                       <  1:3713534/150‑1 (MQ=37)

                                                                                                                              ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgac                       >  1:1729940/1‑150 (MQ=37)

                                                                                                                                 cTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacgac                    >  1:2256301/1‑150 (MQ=37)

                                                                                                                                 cTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacgac                    <  2:1218076/150‑1 (MQ=37)

                                                                                                                                  tGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACa                   >  2:2852598/1‑150 (MQ=37)

                                                                                                                                  tGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACa                   <  2:2816638/150‑1 (MQ=37)

                                                                                                                                  tGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACa                   >  2:2526070/1‑150 (MQ=37)

                                                                                                                                  tGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACa                   >  1:1000955/1‑150 (MQ=37)

                                                                                                                                   gAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACaa                  >  2:4376796/1‑150 (MQ=37)

                                                                                                                                   gAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGGTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACaa                  >  1:1094375/1‑150 (MQ=37)

                                                                                                                                         ggCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTccc                                                                                 <  2:2129936/81‑1 (MQ=38)

                                                                                                                                         ggCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAAt            >  2:939560/1‑150 (MQ=37)

                                                                                                                                             gACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGc        >  1:1928963/1‑150 (MQ=34)

                                                                                                                                             gACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGc        <  2:177188/150‑1 (MQ=34)

                                                                                                                                              aCCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCa       <  2:4172600/150‑1 (MQ=34)

ATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACC  >  NC_002947/549602‑549898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`