BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,584	C→T	14.0%	T174I (ACC→ATC) ‡	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,584	0	C	T	14.0%	507.6 / 60.1	201	T174I (ACC→ATC) ‡	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base C (65/107); new base T (16/12); total (81/120)
Fisher's exact test for biased strand distribution p-value = 6.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

GGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAC  >  NC_002947/554435‑554733

   tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                                                    <  2:3818852/150‑1 (MQ=255)

      gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                                                                 <  2:2924633/150‑1 (MQ=255)

      gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                                                                 >  1:2880940/1‑150 (MQ=255)

         gtcgatcgCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTa                                                                                                                                              >  2:4564276/8‑150 (MQ=255)

            gatcgCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTTCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATcg                                                                                                                                           >  2:3519735/5‑150 (MQ=255)

             gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTGCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTTGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTTGTATcgc                                                                                                                                          <  2:358688/150‑1 (MQ=255)

             gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgc                                                                                                                                          >  2:4159290/1‑150 (MQ=255)

             gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgc                                                                                                                                          <  2:502149/150‑1 (MQ=255)

              tAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgcg                                                                                                                                         >  1:2267132/1‑150 (MQ=255)

                gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                       <  1:1003930/150‑1 (MQ=255)

                gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                       <  1:3385929/150‑1 (MQ=255)

                gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                       <  1:3785547/150‑1 (MQ=255)

                gCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGaa                                                                                                                                       >  2:1446298/1‑150 (MQ=255)

                 cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                      <  1:3967639/150‑1 (MQ=255)

                 cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                      <  2:1261075/150‑1 (MQ=255)

                 cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                      >  1:1803863/1‑150 (MQ=255)

                  tCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAgg                                                                                                                                     >  1:1962528/1‑150 (MQ=255)

                  tCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAgg                                                                                                                                     >  2:3773790/1‑150 (MQ=255)

                    agcagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTc                                                                                                                                   >  2:868543/1‑150 (MQ=255)

                    agcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTc                                                                                                                                   <  1:1806967/150‑1 (MQ=255)

                     gcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCg                                                                                                                                  >  2:3311039/1‑150 (MQ=255)

                      cagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                                                                 <  1:3768189/150‑1 (MQ=255)

                      cagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                                                                 >  2:2864161/1‑150 (MQ=255)

                        gcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgt                                                                                                                               >  2:2350115/1‑150 (MQ=255)

                          tcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgttc                                                                                                                             >  1:3491150/3‑148 (MQ=255)

                          tcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgttc                                                                                                                             >  2:546192/3‑148 (MQ=255)

                          tGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                                                             >  1:1836847/1‑150 (MQ=255)

                            gAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCct                                                                                                                           >  2:727764/1‑150 (MQ=255)

                              aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCctct                                                                                                                         <  1:2120458/150‑1 (MQ=255)

                                    tgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCacac                                                                                                                   >  2:4585211/1‑150 (MQ=255)

                                    tgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCacac                                                                                                                   >  2:1320908/1‑150 (MQ=255)

                                     gtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACAcc                                                                                                                  <  1:964702/150‑1 (MQ=255)

                                        aTGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTg                                                                                                               >  1:3586440/1‑150 (MQ=255)

                                          gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                                                                                                             <  2:2470090/150‑1 (MQ=255)

                                          gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCGGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                                                                                                             >  2:4689146/1‑150 (MQ=255)

                                           ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                            >  1:4459868/1‑150 (MQ=255)

                                           ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                            >  2:3951139/1‑150 (MQ=255)

                                             ccGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGc                                                                                                          <  2:2397366/150‑1 (MQ=255)

                                                      cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                 >  2:3143963/1‑150 (MQ=255)

                                                      cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                 >  1:557208/1‑150 (MQ=255)

                                                      cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                 <  2:4979082/150‑1 (MQ=255)

                                                       aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                <  2:3111208/150‑1 (MQ=255)

                                                       aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                <  2:1170625/150‑1 (MQ=255)

                                                       aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                <  2:301408/150‑1 (MQ=255)

                                                         gAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                                                              <  2:3033872/150‑1 (MQ=255)

                                                          aaGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTac                                                                                             >  1:1393556/1‑150 (MQ=255)

                                                            gCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaa                                                                                           >  1:4879029/1‑150 (MQ=255)

                                                              gTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaaca                                                                                         <  1:225901/150‑1 (MQ=255)

                                                              gTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaaca                                                                                         <  1:1012360/150‑1 (MQ=255)

                                                                   gTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTAc                                                                                    <  1:1603679/150‑1 (MQ=255)

                                                                       aGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                            <  2:37482/122‑1 (MQ=255)

                                                                        gAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTAGTACCGAGTGGAATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGc                                                                               >  2:986160/1‑150 (MQ=255)

                                                                            gCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcac                                                                           >  2:1719370/1‑150 (MQ=255)

                                                                             ccATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcaca                                                                          <  2:4652729/150‑1 (MQ=255)

                                                                              cATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCacac                                                                         <  2:1301472/150‑1 (MQ=255)

                                                                                 gCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcaccac                                                                      >  2:2675759/1‑150 (MQ=255)

                                                                                   gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                                                    >  1:2571904/1‑150 (MQ=255)

                                                                                   gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                                                    <  2:1659554/150‑1 (MQ=255)

                                                                                    tATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTa                                                                   >  2:2412299/1‑150 (MQ=255)

                                                                                            ccAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGa                                                           >  2:2723316/1‑150 (MQ=255)

                                                                                              aaGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATc                                                         <  1:3030023/150‑1 (MQ=255)

                                                                                                gggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCgg                                                       <  1:2726087/150‑1 (MQ=255)

                                                                                                 ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                      <  2:4842002/150‑1 (MQ=255)

                                                                                                  gCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCggcg                                                     <  2:3194735/150‑3 (MQ=255)

                                                                                                    aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                                                   >  2:1916494/1‑150 (MQ=255)

                                                                                                     tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                  <  2:4504061/150‑1 (MQ=255)

                                                                                                     tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                  <  2:3902990/150‑1 (MQ=255)

                                                                                                     tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                  <  1:351426/150‑1 (MQ=255)

                                                                                                        aGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAgg                                               >  2:188173/1‑150 (MQ=255)

                                                                                                           tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGTTCGTGTGCCTCTGCACACCCTGCGTGCCGTTATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                            <  2:3678635/150‑1 (MQ=255)

                                                                                                           tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                            <  2:2316487/150‑1 (MQ=255)

                                                                                                           tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                            <  2:4044494/150‑1 (MQ=255)

                                                                                                           tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                            <  1:3204718/150‑1 (MQ=255)

                                                                                                            ccAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTg                                           <  2:3557654/150‑1 (MQ=255)

                                                                                                             cAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTgg                                          <  2:3887257/150‑1 (MQ=255)

                                                                                                                     cGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaa                                  <  2:1998485/150‑1 (MQ=255)

                                                                                                                      ggTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaaa                                 <  1:2701599/150‑1 (MQ=255)

                                                                                                                      ggTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaaa                                 <  1:2145815/150‑1 (MQ=255)

                                                                                                                        tcgtcTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAggg                               <  1:3311039/150‑1 (MQ=255)

                                                                                                                        tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                 >  1:371137/1‑84 (MQ=255)

                                                                                                                        tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAgg                               >  2:4305277/1‑150 (MQ=255)

                                                                                                                         cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                              <  1:2803032/150‑1 (MQ=255)

                                                                                                                         cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                              <  1:1677322/150‑1 (MQ=255)

                                                                                                                           tcttGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGa                            >  1:4038895/1‑150 (MQ=255)

                                                                                                                           tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                            <  2:2267249/150‑1 (MQ=255)

                                                                                                                           tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                            <  2:2267106/150‑1 (MQ=255)

                                                                                                                            ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                           <  1:2318747/150‑1 (MQ=255)

                                                                                                                            ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTCCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                           <  1:759/150‑1 (MQ=255)

                                                                                                                             tcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                                                              <  1:3901725/82‑1 (MQ=255)

                                                                                                                                gcggcgCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcc                       >  2:335424/1‑145 (MQ=255)

                                                                                                                                 cggCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcct                      <  2:4466956/150‑7 (MQ=255)

                                                                                                                                   gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCGACGAAGCTCACACCACTTCCGGTGTGATCGGTGTGAAGGTTTGGATCTTCAACGGCGAAGTTATtg                    <  2:564618/150‑1 (MQ=255)

                                                                                                                                     gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                          >  1:1306714/1‑126 (MQ=255)

                                                                                                                                      cTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtg                 <  1:4667901/150‑1 (MQ=255)

                                                                                                                                       tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                >  2:2298732/1‑150 (MQ=255)

                                                                                                                                       tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                >  2:789830/1‑150 (MQ=255)

                                                                                                                                        gagaTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTggtggt               >  1:593076/1‑150 (MQ=255)

                                                                                                                                         agaTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTc              >  2:2187161/1‑150 (MQ=255)

                                                                                                                                           aTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGc            <  1:2864161/150‑1 (MQ=255)

                                                                                                                                                     ccGAGTGGTATAGCGAAGGTCGTGTGCCTCTGCACACCCTGCTTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAATGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAc  <  2:713141/150‑1 (MQ=255)

GGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAC  >  NC_002947/554435‑554733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`