BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,585	C→G	13.9%	T174T (ACC→ACG) ‡	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,585	0	C	G	13.9%	570.3 / 72.9	201	T174T (ACC→ACG) ‡	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base C (65/108); new base G (16/12); total (81/120)
Fisher's exact test for biased strand distribution p-value = 6.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 206 total aligned reads displayed.

GTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACT  >  NC_002947/554436‑554734

  tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                                                     <  2:3818852/150‑1 (MQ=255)

     gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                                                                  <  2:2924633/150‑1 (MQ=255)

        gtcgatcgCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTa                                                                                                                                               >  2:4564276/8‑150 (MQ=255)

           gatcgCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTTCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATcg                                                                                                                                            >  2:3519735/5‑150 (MQ=255)

            gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTGCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTTGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTTGTATcgc                                                                                                                                           <  2:358688/150‑1 (MQ=255)

            gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgc                                                                                                                                           >  2:4159290/1‑150 (MQ=255)

            gTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgc                                                                                                                                           <  2:502149/150‑1 (MQ=255)

             tAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgcg                                                                                                                                          >  1:2267106/1‑150 (MQ=255)

             tAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATcgcg                                                                                                                                          >  1:2267249/1‑150 (MQ=255)

               gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                        <  1:343022/150‑1 (MQ=255)

               gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                        <  1:1003930/150‑1 (MQ=255)

               gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                                                        <  1:2219137/150‑1 (MQ=255)

               gCTCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGaa                                                                                                                                        >  2:1446298/1‑150 (MQ=255)

                cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                       >  1:1803863/1‑150 (MQ=255)

                cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                       <  1:3967639/150‑1 (MQ=255)

                cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                                                                                                       <  2:1261075/150‑1 (MQ=255)

                 tCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAgg                                                                                                                                      >  2:3773790/1‑150 (MQ=255)

                   agcagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTc                                                                                                                                    >  2:868543/1‑150 (MQ=255)

                   agcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTc                                                                                                                                    <  1:1806967/150‑1 (MQ=255)

                   agcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTc                                                                                                                                    >  1:3308098/1‑150 (MQ=255)

                    gcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCg                                                                                                                                   >  2:3311039/1‑150 (MQ=255)

                     cagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                                                                  >  2:2864161/1‑150 (MQ=255)

                       gcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgt                                                                                                                                >  2:2350115/1‑150 (MQ=255)

                         tcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgttc                                                                                                                              >  1:3367956/3‑148 (MQ=255)

                         tcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgttc                                                                                                                              >  2:546192/3‑148 (MQ=255)

                         tGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                                                              >  1:1836847/1‑150 (MQ=255)

                           gAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCct                                                                                                                            >  2:727764/1‑150 (MQ=255)

                              cGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTg                                                                                                                         <  1:3501026/150‑1 (MQ=255)

                                   tgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCacac                                                                                                                    >  2:1320908/1‑150 (MQ=255)

                                   tgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCacac                                                                                                                    >  2:4585211/1‑150 (MQ=255)

                                    gtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACAcc                                                                                                                   <  1:964702/150‑1 (MQ=255)

                                         gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                                                                                                              <  2:2470090/150‑1 (MQ=255)

                                         gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCGGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                                                                                                              >  2:4689146/1‑150 (MQ=255)

                                          ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                                                                                                                  <  1:284714/129‑1 (MQ=255)

                                          ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                             >  1:4459868/1‑150 (MQ=255)

                                          ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                             >  2:3951139/1‑150 (MQ=255)

                                            ccGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGc                                                                                                           <  2:2397366/150‑1 (MQ=255)

                                               tCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGa                                                                                                        <  1:2144446/150‑1 (MQ=255)

                                                     cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                  <  2:4979082/150‑1 (MQ=255)

                                                     cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                  >  1:557208/1‑150 (MQ=255)

                                                     cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                                                                  >  2:3143963/1‑150 (MQ=255)

                                                      aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                 >  1:3589883/1‑150 (MQ=255)

                                                      aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                 <  2:301408/150‑1 (MQ=255)

                                                      aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                 <  2:3111208/150‑1 (MQ=255)

                                                      aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                                                                 <  2:1170625/150‑1 (MQ=255)

                                                       tGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACt                                                                                                >  1:2780746/1‑150 (MQ=255)

                                                        gAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                                                               <  2:3033872/150‑1 (MQ=255)

                                                         aaGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTac                                                                                              >  1:1393556/1‑150 (MQ=255)

                                                           gCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaa                                                                                            >  1:4879029/1‑150 (MQ=255)

                                                             gTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTacaaca                                                                                          <  1:1012360/150‑1 (MQ=255)

                                                                  gTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTAc                                                                                     <  1:1603679/150‑1 (MQ=255)

                                                                  gTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTAc                                                                                     >  1:2426357/1‑150 (MQ=255)

                                                                      aGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                             >  1:37482/1‑122 (MQ=255)

                                                                      aGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                                                             <  2:37482/122‑1 (MQ=255)

                                                                       gAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTAGTACCGAGTGGAATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGc                                                                                >  2:986160/1‑150 (MQ=255)

                                                                           gCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcac                                                                            >  1:1940569/1‑150 (MQ=255)

                                                                           gCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcac                                                                            >  2:1719370/1‑150 (MQ=255)

                                                                            ccATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcaca                                                                           <  2:4652729/150‑1 (MQ=255)

                                                                             cATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCacac                                                                          <  2:1301472/150‑1 (MQ=255)

                                                                                gCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcaccac                                                                       >  2:2675759/1‑150 (MQ=255)

                                                                                  gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                                                     <  2:1659554/150‑1 (MQ=255)

                                                                                   tATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTa                                                                    >  2:2412299/1‑150 (MQ=255)

                                                                                       ggTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGgt                                                                <  1:3773790/150‑1 (MQ=255)

                                                                                           ccAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGa                                                            >  2:2723316/1‑150 (MQ=255)

                                                                                                ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                       <  1:2085076/150‑1 (MQ=255)

                                                                                                ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                       <  2:4842002/150‑1 (MQ=255)

                                                                                                 gCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCggcg                                                      <  2:3194735/150‑3 (MQ=255)

                                                                                                   aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                                                    >  2:1916494/1‑150 (MQ=255)

                                                                                                    tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                   <  1:351729/150‑1 (MQ=255)

                                                                                                    tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                   <  2:4504061/150‑1 (MQ=255)

                                                                                                    tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                   <  2:3902990/150‑1 (MQ=255)

                                                                                                       aGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAgg                                                >  2:188173/1‑150 (MQ=255)

                                                                                                          tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGTTCGTGTGCCTCTGCACACCCTGCGTGCCGTTATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                             <  2:3678635/150‑1 (MQ=255)

                                                                                                          tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                             <  2:2316487/150‑1 (MQ=255)

                                                                                                          tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                             <  1:2890724/150‑1 (MQ=255)

                                                                                                          tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                             <  2:4044494/150‑1 (MQ=255)

                                                                                                           ccAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTg                                            <  2:3557654/150‑1 (MQ=255)

                                                                                                            cAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTgg                                           <  2:3887257/150‑1 (MQ=255)

                                                                                                                    cGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaa                                   <  2:1998485/150‑1 (MQ=255)

                                                                                                                       tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAgg                                >  2:4305277/1‑150 (MQ=255)

                                                                                                                        cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                               <  1:1677322/150‑1 (MQ=255)

                                                                                                                          tcttGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGa                             >  1:4038895/1‑150 (MQ=255)

                                                                                                                          tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                             <  2:2267106/150‑1 (MQ=255)

                                                                                                                          tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                             <  2:2267249/150‑1 (MQ=255)

                                                                                                                           cttggcggcGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGag                            <  1:3127818/147‑2 (MQ=255)

                                                                                                                           ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTCCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                            <  1:759/150‑1 (MQ=255)

                                                                                                                            tcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTa                                                                                               <  1:3901725/82‑1 (MQ=255)

                                                                                                                               gcggcgCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcc                        >  2:335424/1‑145 (MQ=255)

                                                                                                                               gcggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTa                        <  1:2872059/150‑1 (MQ=255)

                                                                                                                                cggCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcct                       <  2:4466956/150‑7 (MQ=255)

                                                                                                                                  gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                     <  1:2723316/150‑1 (MQ=255)

                                                                                                                                  gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                     <  1:2630505/150‑1 (MQ=255)

                                                                                                                                  gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCGACGAAGCTCACACCACTTCCGGTGTGATCGGTGTGAAGGTTTGGATCTTCAACGGCGAAGTTATtg                     <  2:564618/150‑1 (MQ=255)

                                                                                                                                    gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                           >  1:1306714/1‑126 (MQ=255)

                                                                                                                                     cTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtg                  <  1:4667901/150‑1 (MQ=255)

                                                                                                                                      tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                 >  2:789830/1‑150 (MQ=255)

                                                                                                                                      tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                 >  2:2298732/1‑150 (MQ=255)

                                                                                                                                       gagaTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTggtggt                >  1:593076/1‑150 (MQ=255)

                                                                                                                                        agaTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTc               >  2:2187161/1‑150 (MQ=255)

                                                                                                                                                    ccGAGTGGTATAGCGAAGGTCGTGTGCCTCTGCACACCCTGCTTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAATGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAc   <  2:713141/150‑1 (MQ=255)

GTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACT  >  NC_002947/554436‑554734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`