BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,660	C→G	6.7%	H199Q (CAC→CAG)	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,660	0	C	G	6.7%	541.4 / 21.0	164	H199Q (CAC→CAG)	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base C (55/98); new base G (4/7); total (59/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 176 total aligned reads displayed.

GCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTAC  >  NC_002947/554511‑554809

gCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcac                                                                                                                                                       >  2:1719370/1‑150 (MQ=255)

gCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcac                                                                                                                                                       >  1:1940569/1‑150 (MQ=255)

  cATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCacac                                                                                                                                                     <  2:1301472/150‑1 (MQ=255)

    tGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacca                                                                                                                                                   <  2:3127441/150‑1 (MQ=255)

    tGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacca                                                                                                                                                   <  2:572486/150‑1 (MQ=255)

        tATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTa                                                                                                                                               >  2:2412299/1‑150 (MQ=255)

                ccAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGa                                                                                                                                       >  2:2723316/1‑150 (MQ=255)

                  aaGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATc                                                                                                                                     <  1:3030023/150‑1 (MQ=255)

                   aGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCg                                                                                                                                    <  2:99448/150‑1 (MQ=255)

                    gggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCgg                                                                                                                                   <  1:2726087/150‑1 (MQ=255)

                     ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                  <  2:1641290/150‑1 (MQ=255)

                      gCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCggcg                                                                                                                                 <  2:3194735/150‑3 (MQ=255)

                       cATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgtgt                                                                                                                                <  2:4614758/150‑1 (MQ=255)

                        aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                                                                                                                               >  2:1916494/1‑150 (MQ=255)

                         tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                              <  2:4504061/150‑1 (MQ=255)

                         tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                              <  1:351426/150‑1 (MQ=255)

                           aaGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAg                                                                                                                            <  2:3944313/150‑1 (MQ=255)

                            aGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAgg                                                                                                                           >  2:188173/1‑150 (MQ=255)

                               tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGTTCGTGTGCCTCTGCACACCCTGCGTGCCGTTATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                        <  2:3678635/150‑1 (MQ=255)

                               tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                        <  2:2316424/150‑1 (MQ=255)

                               tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                        <  2:1674614/150‑1 (MQ=255)

                               tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                        <  1:2890724/150‑1 (MQ=255)

                               tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                        <  2:4044494/150‑1 (MQ=255)

                                ccAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTg                                                                                                                       <  2:3557654/150‑1 (MQ=255)

                                ccAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTg                                                                                                                       <  2:3557085/150‑1 (MQ=255)

                                 cAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTgg                                                                                                                      <  2:3887257/150‑1 (MQ=255)

                                  aGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGa                                                                                                                     <  2:193830/150‑1 (MQ=255)

                                   ggTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGAt                                                                                                                    >  2:875111/1‑150 (MQ=255)

                                        gCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCa                                                                                                               >  2:3089063/1‑150 (MQ=255)

                                         cGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaa                                                                                                              <  2:1998485/150‑1 (MQ=255)

                                          ggTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCaag                                                                                                             <  2:2287628/150‑2 (MQ=255)

                                          ggTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaaa                                                                                                             <  1:2145815/150‑1 (MQ=255)

                                          ggTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaaa                                                                                                             <  1:2701612/150‑1 (MQ=255)

                                            tcgtcTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAggg                                                                                                           <  1:3311039/150‑1 (MQ=255)

                                            tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAgg                                                                                                           >  2:3951318/1‑150 (MQ=255)

                                             cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                                                                                                          <  1:1677322/150‑1 (MQ=255)

                                              gtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCg                                                                                                         <  1:3963661/150‑1 (MQ=255)

                                               tcttGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGa                                                                                                        >  1:4038895/1‑150 (MQ=255)

                                               tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                                                                                                        <  2:2267132/150‑1 (MQ=255)

                                               tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                                                                                                        <  2:2267106/150‑1 (MQ=255)

                                                ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                                                                                                       <  1:2318747/150‑1 (MQ=255)

                                                ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                                                                                                       <  2:3166057/150‑1 (MQ=255)

                                                 tcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                              >  1:5020242/1‑126 (MQ=255)

                                                 tcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                              <  2:5020242/126‑1 (MQ=255)

                                                    gcggcgCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcc                                                                                                   <  2:24053/150‑6 (MQ=255)

                                                     cggCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcct                                                                                                  <  2:4466956/150‑7 (MQ=255)

                                                     cggCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcct                                                                                                  <  2:3305162/150‑7 (MQ=255)

                                                      ggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTAtt                                                                                                 <  2:2496037/150‑1 (MQ=255)

                                                      ggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTAtt                                                                                                 <  2:1722938/150‑1 (MQ=255)

                                                       gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                                                                                                <  2:564557/150‑1 (MQ=255)

                                                       gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                                                                                                <  1:2630505/150‑1 (MQ=255)

                                                         gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                                                                                                      <  2:1306714/126‑1 (MQ=255)

                                                         gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                                                                                                      >  1:1306714/1‑126 (MQ=255)

                                                           tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                                                                                            >  2:789830/1‑150 (MQ=255)

                                                               aTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGc                                                                                        <  1:2864161/150‑1 (MQ=255)

                                                               aTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGc                                                                                        <  2:2880940/150‑1 (MQ=255)

                                                                    tCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCaaga                                                                                   >  2:2431395/1‑150 (MQ=255)

                                                                        aCCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAgaagaa                                                                               <  1:1111423/150‑1 (MQ=255)

                                                                         ccGAGTGGTATAGCGAAGGTCGTGTGCCTCTGCACACCCTGCTTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAATGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAc                                                                              <  2:713141/150‑1 (MQ=255)

                                                                           gAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTg                                                                            >  2:1829985/1‑150 (MQ=255)

                                                                               ggTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAAc                                                                        <  1:100444/150‑1 (MQ=255)

                                                                                   tCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACcaca                                                                    >  1:2789411/1‑150 (MQ=255)

                                                                                    cgcgAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGTAGTTATTGGTGGTCGCCAAGAAGAACTGTAACCACaa                                                                   <  2:4827371/150‑1 (MQ=255)

                                                                                     gcgAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAg                                                                  >  2:2082618/1‑150 (MQ=255)

                                                                                       gAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCa                                                                >  1:3799763/1‑150 (MQ=255)

                                                                                       gAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTCACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCa                                                                >  1:1216855/1‑150 (MQ=255)

                                                                                          ggTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCa                                                             >  1:5063600/1‑150 (MQ=255)

                                                                                            tCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                  <  1:17212/79‑1 (MQ=255)

                                                                                              gtgtGCCACTGCACACCATGAGTGCCTATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAgcgc                                                         >  2:122519/1‑150 (MQ=255)

                                                                                               tgtgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGcc                                                        >  2:2541856/1‑150 (MQ=255)

                                                                                               tgtgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGcc                                                        >  1:3021150/1‑150 (MQ=255)

                                                                                                 tgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACAACTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTc                                                      <  1:4911358/150‑1 (MQ=255)

                                                                                                  gCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCg                                                     <  2:753645/150‑1 (MQ=255)

                                                                                                    ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTa                                                   >  1:4450452/1‑150 (MQ=255)

                                                                                                    ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTa                                                   >  1:548960/1‑150 (MQ=255)

                                                                                                    ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTa                                                   >  2:5031451/1‑150 (MQ=255)

                                                                                                     tctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaa                                                  <  2:1803863/150‑1 (MQ=255)

                                                                                                        gCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaaaaa                                               <  1:727764/150‑1 (MQ=255)

                                                                                                        gCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaaaaa                                               >  2:53703/1‑150 (MQ=255)

                                                                                                            acCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgct                                           <  2:4459868/150‑1 (MQ=255)

                                                                                                            acCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgct                                           <  1:1928389/150‑1 (MQ=255)

                                                                                                              ccTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgctgc                                         <  1:4585211/150‑1 (MQ=255)

                                                                                                              ccTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgctgc                                         <  1:1320908/150‑1 (MQ=255)

                                                                                                                 gCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCtaa                                      <  2:100816/150‑1 (MQ=255)

                                                                                                                       cGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAgg                                <  2:2571904/150‑1 (MQ=255)

                                                                                                                       cGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAgg                                >  2:4790856/1‑150 (MQ=255)

                                                                                                                            tCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTAc                           <  1:4159290/150‑1 (MQ=255)

                                                                                                                              gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCTGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                         >  1:2411082/1‑150 (MQ=255)

                                                                                                                              gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                         >  1:2887378/1‑150 (MQ=255)

                                                                                                                              gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                         <  2:4863801/150‑1 (MQ=255)

                                                                                                                               aCTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGcc                        >  1:1327770/1‑150 (MQ=255)

                                                                                                                                     acacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGt                  >  1:370056/1‑150 (MQ=255)

                                                                                                                                     acacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGt                  >  1:3489586/1‑150 (MQ=255)

                                                                                                                                     acacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGt                  <  1:868543/150‑1 (MQ=255)

                                                                                                                                      cacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGtt                 <  1:3143963/150‑1 (MQ=255)

                                                                                                                                           aCGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAAc            >  1:5063647/1‑150 (MQ=255)

                                                                                                                                            cGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAAcc           <  1:2412299/150‑1 (MQ=255)

                                                                                                                                                 cTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGc      <  1:875111/150‑1 (MQ=255)

                                                                                                                                                  tCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCg     >  1:4257655/1‑150 (MQ=255)

                                                                                                                                                    acacAAATTACGGTGTGATCGGTGTGAAGGTTTTTATCTTCAAAGGCGAAGTTATTGGTGGTCGCGTATAAGTAGTGAAACCACAAGCACCAGCGGGTCGTAAAAAATCTGATATGTGAGGGGTAGGCCAATTGTTGCAACaaaatggta   >  2:4302057/1‑145 (MQ=255)

GCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTAC  >  NC_002947/554511‑554809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`