Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 554,664 A→G 6.6% T201A (ACT→GCT)  rpsC → 30S ribosomal protein S3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947554,6640AG 6.6% 518.6 / 16.5 167T201A (ACT→GCT) rpsC30S ribosomal protein S3
Reads supporting (aligned to +/- strand):  ref base A (58/98);  new base G (4/7);  total (62/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 175 total aligned reads displayed.

TGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTACAAA  >  NC_002947/554515‑554812
                                                                                                                                                     |                                                                                                                                                    
tGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacca                                                                                                                                                      <  2:572486/150‑1 (MQ=255)
 gCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcaccac                                                                                                                                                     >  2:2675759/1‑150 (MQ=255)
   gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                                                                                                                                   <  2:1659554/150‑1 (MQ=255)
   gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                                                                                                                                   >  1:2571904/1‑150 (MQ=255)
            ccAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGa                                                                                                                                          >  2:2723316/1‑150 (MQ=255)
               aGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCg                                                                                                                                       <  2:99448/150‑1 (MQ=255)
                 ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                     <  2:4842002/150‑1 (MQ=255)
                 ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                     <  1:2085076/150‑1 (MQ=255)
                 ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                     <  2:1641290/150‑1 (MQ=255)
                  gCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCggcg                                                                                                                                    <  2:4209924/150‑3 (MQ=255)
                    aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                                                                                                                                  >  2:1916494/1‑150 (MQ=255)
                     tCAAGATCGAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAGCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCAGACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTCCGGTGTGATCGGTGTGa                                                                                                                                 <  2:4504044/150‑1 (MQ=255)
                     tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                                 <  1:351426/150‑1 (MQ=255)
                     tCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                                 <  2:3902990/150‑1 (MQ=255)
                        aGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAgg                                                                                                                              >  2:188173/1‑150 (MQ=255)
                           tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt                                                                                                                           <  2:2316424/150‑1 (MQ=255)
                            ccAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTg                                                                                                                          <  2:3557654/150‑1 (MQ=255)
                               ggTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGAt                                                                                                                       >  2:875111/1‑150 (MQ=255)
                                    gCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCa                                                                                                                  >  2:3089063/1‑150 (MQ=255)
                                     cGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaa                                                                                                                 <  2:1998485/150‑1 (MQ=255)
                                      ggTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCaag                                                                                                                <  2:2287628/150‑2 (MQ=255)
                                      ggTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCaaa                                                                                                                <  1:2701599/150‑1 (MQ=255)
                                        tcgtcTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAggg                                                                                                              <  1:3311039/150‑1 (MQ=255)
                                        tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAgg                                                                                                              >  2:3951318/1‑150 (MQ=255)
                                        tcgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAgg                                                                                                              >  2:4305277/1‑150 (MQ=255)
                                         cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                                                                                                             <  1:1677322/150‑1 (MQ=255)
                                         cgtcTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGc                                                                                                             <  1:2803032/150‑1 (MQ=255)
                                           tcttGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGa                                                                                                           >  1:4038895/1‑150 (MQ=255)
                                           tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                                                                                                           <  2:2267249/150‑1 (MQ=255)
                                           tctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGa                                                                                                           <  2:2267106/150‑1 (MQ=255)
                                            ctcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGaa                                                                                                          <  2:3166057/150‑1 (MQ=255)
                                             tcGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGa                                                                                                                                 <  2:5020242/126‑1 (MQ=255)
                                                gcggcgCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcc                                                                                                      >  2:335424/1‑145 (MQ=255)
                                                gcggcgCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcc                                                                                                      <  2:24053/150‑6 (MQ=255)
                                                gcggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTa                                                                                                      <  1:2872059/150‑1 (MQ=255)
                                                 cggCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTGGGTATTCAAGGGCGagatcct                                                                                                     <  2:4466956/150‑7 (MQ=255)
                                                  ggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTAtt                                                                                                    <  2:2496037/150‑1 (MQ=255)
                                                  ggTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTAtt                                                                                                    <  2:1722938/150‑1 (MQ=255)
                                                   gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                                                                                                   <  1:2723316/150‑1 (MQ=255)
                                                   gTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtg                                                                                                   <  2:564557/150‑1 (MQ=255)
                                                     gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                                                                                                         >  1:1306714/1‑126 (MQ=255)
                                                     gCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGTGCCGATATCGACTACGGTACGGCAGAAGCGCAGACCGCTTACGGCATCTGCGGCGTGAAGGTTTgg                                                                                                                         <  2:1306714/126‑1 (MQ=255)
                                                       tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                                                                                               >  2:789830/1‑150 (MQ=255)
                                                       tGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGAACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATtggtgg                                                                                               >  2:789685/1‑150 (MQ=255)
                                                        gagaTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTggtggt                                                                                              >  1:593076/1‑150 (MQ=255)
                                                           aTTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGc                                                                                           <  2:2880940/150‑1 (MQ=255)
                                                                tCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCaaga                                                                                      >  2:2431395/1‑150 (MQ=255)
                                                                    aCCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAgaagaa                                                                                  <  1:1111423/150‑1 (MQ=255)
                                                                     ccGAGTGGTCGCGCGAAGGTCGTGTGCCTCTGCACCCCCTGCGTGCCGAGATCGACTACAACACCTACGAAGCTCACACCACTTACGGGGTGATCGGTGTGAAGGGTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCACGAAGAAc                                                                                 >  2:4576269/1‑150 (MQ=255)
                                                                     ccGAGTGGTATAGCGAAGGTCGTGTGCCTCTGCACACCCTGCTTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAATGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAAc                                                                                 <  2:713141/150‑1 (MQ=255)
                                                                       gAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTg                                                                               >  2:1829985/1‑150 (MQ=255)
                                                                           ggTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAAc                                                                           <  1:100444/150‑1 (MQ=255)
                                                                               tCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACcaca                                                                       >  1:2789411/1‑150 (MQ=255)
                                                                                 gcgAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAg                                                                     >  2:2082618/1‑150 (MQ=255)
                                                                                   gAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCa                                                                   >  1:3799763/1‑150 (MQ=255)
                                                                                   gAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTCACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCa                                                                   >  1:1216855/1‑150 (MQ=255)
                                                                                        tCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                                                                                                                                     <  1:17212/79‑1 (MQ=255)
                                                                                        tCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAgc                                                              <  2:1962528/150‑1 (MQ=255)
                                                                                          gtgtGCCACTGCACACCATGAGTGCCTATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAgcgc                                                            >  2:122519/1‑150 (MQ=255)
                                                                                           tgtgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGcc                                                           >  1:3021150/1‑150 (MQ=255)
                                                                                           tgtgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGcc                                                           >  2:2541856/1‑150 (MQ=255)
                                                                                            gtgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCt                                                          <  1:3446098/150‑1 (MQ=255)
                                                                                             tgCCTCTGCACACCCTGCGTGCCGATATCGACTACAACAACTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTc                                                         <  1:4911358/150‑1 (MQ=255)
                                                                                               ccTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGt                                                       <  1:3951139/150‑1 (MQ=255)
                                                                                                ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAAcc                                                                          >  1:932734/1‑130 (MQ=255)
                                                                                                ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTa                                                      >  2:5031451/1‑150 (MQ=255)
                                                                                                ctctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTa                                                      >  1:548960/1‑150 (MQ=255)
                                                                                                 tctGCACACCCTGCTTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaa                                                     >  2:3193953/1‑150 (MQ=255)
                                                                                                 tctGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaa                                                     <  2:1803902/150‑1 (MQ=255)
                                                                                                    gCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTaaaaa                                                  <  1:727764/150‑1 (MQ=255)
                                                                                                       cacCGTGCGTGCCGATATCGCCTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTTTTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCTCCTCGTAAAAAAgc                                               <  1:3028212/150‑1 (MQ=255)
                                                                                                        acCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgct                                              <  1:1928389/150‑1 (MQ=255)
                                                                                                        acCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgct                                              <  2:557208/150‑1 (MQ=255)
                                                                                                         cccTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgctg                                             <  2:1706100/150‑1 (MQ=255)
                                                                                                          ccTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgctgc                                            <  1:4585211/150‑1 (MQ=255)
                                                                                                          ccTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAgctgc                                            <  1:1320908/150‑1 (MQ=255)
                                                                                                             gCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCtaa                                         <  2:100816/150‑1 (MQ=255)
                                                                                                                tGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTTCGGTGTGATCTGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCTAGAAGATCTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCtaagta                                      <  2:3589883/150‑1 (MQ=255)
                                                                                                                tGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCtaagta                                      <  1:2326231/150‑1 (MQ=255)
                                                                                                                   cGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAgg                                   >  2:4790856/1‑150 (MQ=255)
                                                                                                                     atatCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAgggg                                 >  2:3284856/1‑150 (MQ=255)
                                                                                                                        tCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTAc                              <  1:4159290/150‑1 (MQ=255)
                                                                                                                          gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                            <  2:2426357/150‑1 (MQ=255)
                                                                                                                          gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                            >  1:2887390/1‑150 (MQ=255)
                                                                                                                          gACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGc                            <  2:4863801/150‑1 (MQ=255)
                                                                                                                           aCTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGcc                           >  1:1327770/1‑150 (MQ=255)
                                                                                                                               caacaCCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAAt                       >  2:3998086/1‑150 (MQ=255)
                                                                                                                                 acacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGt                     <  1:868543/150‑1 (MQ=255)
                                                                                                                                 acacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGt                     >  1:370056/1‑150 (MQ=255)
                                                                                                                                  cacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGtt                    <  1:4365424/150‑1 (MQ=255)
                                                                                                                                  cacCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGtt                    <  1:3143963/150‑1 (MQ=255)
                                                                                                                                      tACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCaa                >  1:4968967/1‑150 (MQ=255)
                                                                                                                                       aCGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAAc               >  1:5063647/1‑150 (MQ=255)
                                                                                                                                        cGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAAcc              <  1:2412299/150‑1 (MQ=255)
                                                                                                                                              tCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCg        >  1:4257655/1‑150 (MQ=255)
                                                                                                                                                acacCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCgga      >  1:5022990/1‑148 (MQ=255)
                                                                                                                                                acacCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTa      >  1:2212982/1‑150 (MQ=255)
                                                                                                                                                 caccacTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTAc     >  2:370453/1‑150 (MQ=255)
                                                                                                                                                  accacTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTACa    <  1:4689146/150‑1 (MQ=255)
                                                                                                                                                    cacTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTACaaa  >  1:3035913/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTCAAAGGCGAAGTTATTGGTGGTCGCCAAGAAGAACTGAAACCACAAGCACCAGCGCCTCGTAAAAAAGCTGCTAAGTAAGGGGTACGCCAAATGTTGCAACCAAAGCGTACAAA  >  NC_002947/554515‑554812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑