BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	711,085	G→C	5.2%	G294R (GGT→CGT) ‡	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	711,085	0	G	C	5.2%	574.2 / 14.4	172	G294R (GGT→CGT) ‡	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (81/82); new base C (5/4); total (86/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 173 total aligned reads displayed.

TCCCGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGC  >  NC_002947/710936‑711231

tCCCGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGCGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAg                                                                                                                                                    >  1:1037901/1‑150 (MQ=255)

   cGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGtt                                                                                                                                                 >  1:2203796/1‑150 (MQ=255)

    ggCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTc                                                                                                                                                <  1:526619/150‑1 (MQ=255)

           cAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                         <  1:719496/150‑1 (MQ=255)

            aGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                        <  2:3546085/150‑1 (MQ=255)

            aGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTgg                                                                                                                                        >  2:178109/1‑150 (MQ=255)

            aGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTgg                                                                                                                                        >  1:236036/1‑150 (MQ=255)

               ctctGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGc                                                                                                                                     <  1:3036279/150‑1 (MQ=255)

               ctctGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGc                                                                                                                                     <  1:2486503/150‑1 (MQ=255)

                tctGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCt                                                                                                                                    >  2:1052532/1‑150 (MQ=255)

                  tGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGa                                                                                                                                  <  2:165000/150‑1 (MQ=255)

                    aaCATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATc                                                                                                                                >  1:4965786/1‑150 (MQ=255)

                    aaCATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATc                                                                                                                                <  2:70137/150‑1 (MQ=255)

                     aCATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCa                                                                                                                               <  1:2107091/150‑1 (MQ=255)

                      cctCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCaa                                                                                                                              <  2:3972744/148‑1 (MQ=255)

                           aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                         >  1:3653760/1‑150 (MQ=255)

                           aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                         <  1:1223513/150‑1 (MQ=255)

                           aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                         <  1:3974014/150‑1 (MQ=255)

                           aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCGGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                         <  2:4751835/150‑1 (MQ=255)

                               ggAATTCAAGTACGCCCTGGACGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCa                                                                                                                     <  2:3486803/150‑1 (MQ=255)

                                     cAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGtt                                                                                                               <  2:146660/150‑1 (MQ=255)

                                     cAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGtt                                                                                                               <  1:44144/150‑1 (MQ=255)

                                      caGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTCGTCGAGGCGAGCCTGAAGCGCTACAACCGGCACGGTTCGGTTGTTGCCAGCGCCCACGGTTCGGCGCAGGAGCTGCGCAACTTCCGGCATCCGGTc                                                                                                              >  2:3365762/2‑150 (MQ=255)

                                      aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTc                                                                                                              >  1:662729/1‑150 (MQ=255)

                                      aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTGGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGATc                                                                                                              >  2:1989240/1‑150 (MQ=255)

                                               cTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGc                                                                                                     <  2:3675671/150‑1 (MQ=255)

                                                 ggTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCt                                                                                                   <  2:4307954/150‑1 (MQ=255)

                                                     gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCg                                                                                               >  2:3185333/1‑150 (MQ=255)

                                                     gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCg                                                                                               >  2:4092516/1‑150 (MQ=255)

                                                      acacCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGc                                                                                              <  1:3432943/150‑1 (MQ=255)

                                                        acCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCa                                                                                            >  2:1097496/1‑150 (MQ=255)

                                                          cGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAAt                                                                                          >  1:515796/1‑150 (MQ=255)

                                                                    ctgctgGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                         >  2:5037301/1‑109 (MQ=255)

                                                                    ctgctgGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGcc                                                                                <  1:1463865/150‑1 (MQ=255)

                                                                       ctggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGAc                                                                             >  2:2075952/1‑150 (MQ=255)

                                                                       ctggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTAGGTTGTTGACAGCGCACAAGGTTCGGCTCTGGAGCTGATCAACTTCCGACATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGAc                                                                             >  2:2503760/1‑150 (MQ=255)

                                                                        tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACt                                                                            >  1:4629854/1‑150 (MQ=255)

                                                                        tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACt                                                                            >  2:1174967/1‑150 (MQ=255)

                                                                           tgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACg                                                                         <  2:4965786/150‑1 (MQ=255)

                                                                              tggctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCg                                                                      >  2:333556/1‑150 (MQ=255)

                                                                                  tgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCt                                                                  >  1:3891818/1‑150 (MQ=255)

                                                                                  tgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAAACTACCTGGCCGACTACGTCGAGCt                                                                  >  2:3414687/1‑150 (MQ=255)

                                                                                    aaGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTgg                                                                <  1:1997086/150‑1 (MQ=255)

                                                                                     agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTggg                                                               <  2:2203796/150‑1 (MQ=255)

                                                                                         cTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGcc                                                           <  1:798789/150‑1 (MQ=255)

                                                                                          tGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCGGGGCGCCg                                                          >  2:2363042/1‑150 (MQ=255)

                                                                                            gTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGt                                                        >  2:991382/1‑150 (MQ=255)

                                                                                             tCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTa                                                       >  2:897365/1‑150 (MQ=255)

                                                                                              cGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTAc                                                      <  2:3891818/150‑1 (MQ=255)

                                                                                               gAGTCGTGCCTGAAGCGCTACAACCTGGAAGGGTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGGTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTAcc                                                     >  2:2999492/1‑150 (MQ=255)

                                                                                                aGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCg                                                    <  1:1423932/150‑1 (MQ=255)

                                                                                                     tGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                               <  2:3014018/150‑1 (MQ=255)

                                                                                                     tGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                               <  2:4623761/150‑1 (MQ=255)

                                                                                                      gCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtgg                                              >  1:5095273/1‑150 (MQ=255)

                                                                                                        ccgccgcgcTACACCCGGGACGGGGCGGGGGTGGCCAGCGCCCACGGTTCGGCGCGGGAGCGGATCAACTTCCGTCCTCCGTTCTACGATCGCCGGTCGCCAATCTACCGGGCCGCCGACGTCGAGCTGGGCGCCGGTACCGGCgtggtg                                            <  2:1037901/145‑1 (MQ=255)

                                                                                                         tGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGc                                           >  1:4207527/1‑150 (MQ=255)

                                                                                                                cTACACCCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTc                                    <  2:1610971/150‑1 (MQ=255)

                                                                                                                cTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTCCCGGCGTGGTGCCTTCCTc                                    >  1:4982989/1‑150 (MQ=255)

                                                                                                                 tACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCg                                   >  2:1550396/1‑150 (MQ=255)

                                                                                                                 tACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCg                                   >  2:1856630/1‑150 (MQ=255)

                                                                                                                 tACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCg                                   >  2:429986/1‑150 (MQ=255)

                                                                                                                 tACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCg                                   >  1:1249129/1‑150 (MQ=255)

                                                                                                                    aaCCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCa                                <  2:4937853/150‑1 (MQ=255)

                                                                                                                    aaCCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCa                                >  1:2652668/1‑150 (MQ=255)

                                                                                                                    aaCCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCa                                >  2:2697112/1‑150 (MQ=255)

                                                                                                                      ccTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGc                              >  2:2802910/1‑150 (MQ=255)

                                                                                                                       cTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGCGGTGCATTCCTCGCCAGcc                             >  2:3735437/1‑150 (MQ=255)

                                                                                                                        tGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCt                            >  1:1715096/1‑150 (MQ=255)

                                                                                                                        tGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCt                            >  1:3274225/1‑150 (MQ=255)

                                                                                                                         ggAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCa                                >  1:1973413/1‑145 (MQ=255)

                                                                                                                              gTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTg                      >  2:3140237/1‑150 (MQ=255)

                                                                                                                               ttCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGa                     <  2:4360350/150‑1 (MQ=255)

                                                                                                                                   gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgac                 >  1:2450816/1‑150 (MQ=255)

                                                                                                                                   gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgac                 <  1:2300686/150‑1 (MQ=255)

                                                                                                                                   gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgac                 <  1:4198427/150‑1 (MQ=255)

                                                                                                                                   gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgac                 >  2:190149/1‑150 (MQ=255)

                                                                                                                                      gttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgtcgtc              <  1:857218/150‑3 (MQ=255)

                                                                                                                                      agctccAGCGCCGAACCTTGGGCGCTGGCGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggt                                             <  2:3601544/115‑1 (MQ=38)

                                                                                                                                      agctccAGCGCCGAACCTTGGGCGCTGGCGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggt                                             >  1:3601544/5‑119 (MQ=38)

                                                                                                                                       ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt             >  2:1308684/1‑150 (MQ=255)

                                                                                                                                       ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt             >  1:4156815/1‑150 (MQ=255)

                                                                                                                                         tccAGCGCCGAACCTTGGGCGCTGGATCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc           >  1:894756/2‑150 (MQ=255)

                                                                                                                                         tccAGCGCCGAACCTTGGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc           >  1:4769338/2‑150 (MQ=255)

                                                                                                                                         gCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc           <  2:5104725/150‑1 (MQ=255)

                                                                                                                                         gCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc           <  2:1180453/150‑1 (MQ=255)

                                                                                                                                         gCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc           <  1:802995/150‑1 (MQ=255)

                                                                                                                                          ccAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCg          >  1:2878212/1‑150 (MQ=255)

                                                                                                                                          ccAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCg          >  2:4165997/1‑150 (MQ=255)

                                                                                                                                           cAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGt         >  1:4276008/1‑150 (MQ=255)

                                                                                                                                            aGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTc        <  1:348106/150‑1 (MQ=255)

                                                                                                                                            aGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTc        >  2:1563177/1‑150 (MQ=255)

                                                                                                                                             gcgcCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCa       <  2:711450/150‑1 (MQ=255)

                                                                                                                                              cgcCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCAc      <  1:1860907/150‑1 (MQ=255)

                                                                                                                                               gcCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCAcc     >  1:1135871/1‑150 (MQ=255)

                                                                                                                                                 ccAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTg   >  1:1610407/1‑150 (MQ=255)

                                                                                                                                                 ccAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTg   >  2:3845204/1‑150 (MQ=255)

                                                                                                                                                 ccAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTg   >  2:2305281/1‑150 (MQ=255)

                                                                                                                                                 ccAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTg   >  1:4294435/1‑150 (MQ=255)

                                                                                                                                                 ccAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTg   >  1:1066434/1‑150 (MQ=255)

                                                                                                                                                  cAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGc  <  1:2316220/150‑1 (MQ=255)

TCCCGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGC  >  NC_002947/710936‑711231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`