Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 727,319 A→T 5.7% W6R (TGG→AGG) ‡ PP_0621 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947727,3190AT 5.7% 572.5 / 12.5 173W6R (TGG→AGG) ‡PP_0621hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (80/82);  new base T (4/6);  total (85/88)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

GCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCAT  >  NC_002947/727172‑727467
                                                                                                                                                   |                                                                                                                                                    
gCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAg                                                                                                                                                    <  2:2828345/150‑1 (MQ=255)
      gctggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTcc                                                                                                                                              <  2:2345091/150‑1 (MQ=255)
      gctggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTcc                                                                                                                                              <  1:2896349/150‑1 (MQ=255)
        tggctgAGTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGTTCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTGTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCt                                                                                                                                            >  2:1397013/1‑150 (MQ=255)
        tggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCt                                                                                                                                            <  2:2536402/150‑1 (MQ=255)
        tggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCt                                                                                                                                            <  1:3518828/150‑1 (MQ=255)
         ggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCtt                                                                                                                                           >  2:3292616/1‑150 (MQ=255)
         ggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCtt                                                                                                                                           <  1:3793965/150‑1 (MQ=255)
          gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                          >  1:290768/1‑150 (MQ=255)
          gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                          <  2:127344/150‑1 (MQ=255)
          gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                          >  1:2148375/1‑150 (MQ=255)
           ctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttc                                                                                                                                         <  1:1803990/150‑1 (MQ=255)
            tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  1:3782357/150‑1 (MQ=255)
            tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  2:1158058/150‑1 (MQ=255)
            tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  2:3445511/150‑1 (MQ=255)
             gACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcat                                                                                                                                       >  2:1106997/1‑150 (MQ=255)
              aCTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcatt                                                                                                                                      <  1:3545564/150‑1 (MQ=255)
               cTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattc                                                                                                                                     <  1:1890280/150‑1 (MQ=255)
                tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    >  2:15036/1‑150 (MQ=255)
                tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    >  2:2073478/1‑150 (MQ=255)
                  tGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATGGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCAcc                                                                                                                                  <  2:2352973/150‑1 (MQ=255)
                        tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                            <  2:1221972/150‑1 (MQ=255)
                         gcgcCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAg                                                                                                                           <  2:2083743/150‑1 (MQ=255)
                           gcCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGt                                                                                                                         <  1:52214/150‑1 (MQ=255)
                             cacaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGGGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCa                                                                                                                       <  1:1229293/150‑1 (MQ=255)
                              acaGCCAGACCGCGGCGGCGATCAGGGCGATCCAGAACAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAg                                                                                                                      <  2:4915338/150‑1 (MQ=255)
                                  ccAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTGGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGa                                                                                                                  >  2:4754709/1‑150 (MQ=255)
                                   cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAATAGGGAGGCCCAAGGCCTCCCGTGCCTTCACCGCTGAAGGTCAGTctta                                                                                                                 >  1:1534897/1‑147 (MQ=255)
                                   cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGaa                                                                                                                 <  1:2052816/150‑1 (MQ=255)
                                   cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGaa                                                                                                                 >  1:1580568/1‑150 (MQ=255)
                                   cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACACGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGaa                                                                                                                 >  1:1168451/1‑150 (MQ=255)
                                    aGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAAc                                                                                                                >  1:426418/1‑150 (MQ=255)
                                     gAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGGACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACa                                                                                                               >  1:1132885/1‑150 (MQ=255)
                                      aaCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAg                                                                                                              <  2:499626/150‑1 (MQ=255)
                                       aCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGa                                                                                                             <  2:5047436/150‑1 (MQ=255)
                                       aCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGa                                                                                                             <  1:4007231/150‑1 (MQ=255)
                                        cgcgGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                            >  2:4092145/1‑150 (MQ=255)
                                        cgcgGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                            >  1:4294001/1‑150 (MQ=255)
                                         gcggcggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAcc                                                                                                           >  2:3368896/1‑150 (MQ=255)
                                            gcggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCaaa                                                                                                        <  1:4092145/150‑1 (MQ=255)
                                            gcggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCaaa                                                                                                        <  1:828111/150‑1 (MQ=255)
                                             cggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAg                                                                                                       >  2:2705282/1‑150 (MQ=255)
                                              ggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAgg                                                                                                      >  2:1390890/1‑150 (MQ=255)
                                                cgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTc                                                                                                    <  2:3573828/150‑1 (MQ=255)
                                                 gATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCa                                                                                                   >  2:4342240/1‑150 (MQ=255)
                                                       ggCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCtttt                                                                                                                                         <  1:3247481/106‑2 (MQ=255)
                                                       ggCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGc                                                                                             <  2:4402275/150‑1 (MQ=255)
                                                          gATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGa                                                                                          >  1:1440771/1‑150 (MQ=255)
                                                              cAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCa                                                                                      <  1:1106997/150‑1 (MQ=255)
                                                               aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACATTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                     >  2:1119896/1‑150 (MQ=255)
                                                                  aaaGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGaa                                                                                  <  1:4071957/150‑1 (MQ=255)
                                                                        ggCGAACCATGGTGTCCAGATTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCt                                                                            <  2:3612296/150‑1 (MQ=255)
                                                                         gCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCtt                                                                           >  1:73825/1‑150 (MQ=255)
                                                                          cGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCttt                                                                          <  1:3456053/150‑1 (MQ=255)
                                                                          cGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCttt                                                                          <  1:883128/150‑1 (MQ=255)
                                                                             aCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtctc                                                                       <  1:2575861/150‑1 (MQ=255)
                                                                             aCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtctc                                                                       <  1:21612/150‑1 (MQ=255)
                                                                               cATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCgg                                                                     >  1:454092/1‑150 (MQ=255)
                                                                                   gtgtCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGcttc                                                                 <  2:1580568/150‑1 (MQ=255)
                                                                                      tCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCttt                                                              <  1:3303688/150‑1 (MQ=255)
                                                                                        cAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCg                                                            <  1:1777693/150‑1 (MQ=255)
                                                                                         aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                           <  1:578486/150‑1 (MQ=255)
                                                                                           cTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGc                                                         <  2:3963370/150‑1 (MQ=255)
                                                                                                 gATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTATCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAAAGGTCTTTCTCGGCTTCTATCGGGCCTTCCg                                                   >  2:2711855/1‑150 (MQ=255)
                                                                                                  aTTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCgg                                                  >  2:4669885/1‑150 (MQ=255)
                                                                                                    tGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCa                                                >  2:3797814/1‑150 (MQ=255)
                                                                                                     gaagaaGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCAc                                               <  1:2786081/150‑1 (MQ=255)
                                                                                                      aagaagCCAACAGTCTAGGACGCGGCCAATAAAAATGGGAGCCCCAAGCCCTCCCTTGAATACCCCGCTGACGTTACGGCGAACCGACCAACGGGCAGGAAGGGGAACCAGGAACGGTATTACACGGATACTTACGGGCGTGCCGGCACg                                              >  2:4984482/1‑150 (MQ=255)
                                                                                                       agaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGa                                             <  2:2117268/150‑1 (MQ=255)
                                                                                                       agaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGa                                             <  1:4944214/150‑1 (MQ=255)
                                                                                                        gaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAt                                            <  2:3152508/150‑1 (MQ=255)
                                                                                                         aagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGCACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAtt                                           <  2:4462026/150‑1 (MQ=255)
                                                                                                         aagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAtt                                           <  1:4128529/150‑1 (MQ=255)
                                                                                                         aagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAtt                                           <  1:1742057/150‑1 (MQ=255)
                                                                                                           gCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCa                                                                                      >  2:4341026/1‑105 (MQ=255)
                                                                                                           gCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCa                                                                                      <  1:4341026/105‑1 (MQ=255)
                                                                                                              aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTc                                      >  1:3416071/1‑150 (MQ=255)
                                                                                                                cAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGc                                    >  2:4364061/1‑150 (MQ=255)
                                                                                                                  gTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCg                                  <  2:2946812/150‑1 (MQ=255)
                                                                                                                     tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtc                               >  2:1046868/1‑150 (MQ=255)
                                                                                                                     tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtc                               <  1:2777379/150‑1 (MQ=255)
                                                                                                                      aGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtct                              >  2:2706725/1‑150 (MQ=255)
                                                                                                                      aGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtct                              >  2:2272741/1‑150 (MQ=255)
                                                                                                                        gACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCg                            <  2:535041/150‑1 (MQ=255)
                                                                                                                        gACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCg                            <  2:2515688/150‑1 (MQ=255)
                                                                                                                           gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCGTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCt                         >  1:1013352/1‑150 (MQ=255)
                                                                                                                             ggCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGACCGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCttt                       >  2:1787151/1‑150 (MQ=255)
                                                                                                                             ggCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCttt                       >  1:3218718/1‑150 (MQ=255)
                                                                                                                             ggCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGGCAGGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCATCCGCCACGAGTGGGTCGCCGTTCTCGTCttt                       >  1:3758334/1‑150 (MQ=255)
                                                                                                                              gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  2:4294001/150‑1 (MQ=255)
                                                                                                                              gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  2:3067797/150‑1 (MQ=255)
                                                                                                                              gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  1:1824836/150‑1 (MQ=255)
                                                                                                                              gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  1:1069628/150‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgca                  >  2:3509982/1‑150 (MQ=255)
                                                                                                                                     aaaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcagca               >  1:5074945/1‑150 (MQ=255)
                                                                                                                                        aaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGct            >  2:33190/1‑150 (MQ=255)
                                                                                                                                           ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc         >  1:4821566/1‑150 (MQ=255)
                                                                                                                                           ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc         >  1:4821545/1‑150 (MQ=255)
                                                                                                                                             aGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGACTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTcgc       <  2:3842599/150‑1 (MQ=255)
                                                                                                                                                 ccAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCa   >  2:1811150/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCAT  >  NC_002947/727172‑727467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑