BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,416	A→C	6.2%	P651P (CCA→CCC)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,416	0	A	C	6.2%	520.6 / 17.8	160	P651P (CCA→CCC)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base A (77/73); new base C (2/8); total (79/81)
Fisher's exact test for biased strand distribution p-value = 9.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 167 total aligned reads displayed.

TGTTCCTCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAC  >  NC_002947/732268‑732562

tgttcctCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAg                                                                                                                                                   <  1:3606142/150‑1 (MQ=255)

 gttcctCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAgg                                                                                                                                                  <  2:4962302/150‑1 (MQ=255)

  ttcctCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGc                                                                                                                                                 >  2:1955447/1‑150 (MQ=255)

  ttcctCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGc                                                                                                                                                 >  2:5041745/1‑150 (MQ=255)

  gtcctCGGCCCGTCCGGTGTGGGCAAGACCGTACTGTGCAAGGCGCTGGCCGTGTTCCTGTTCGACACCGAAGAGGCGATGGTGGGCATCGACATGTGCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGTTCGGTGCACCACCAGGc                                                                                                                                                 <  1:1703716/149‑1 (MQ=255)

      tCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATg                                                                                                                                             >  1:595353/1‑150 (MQ=255)

      tCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATg                                                                                                                                             >  1:487844/1‑150 (MQ=255)

       cGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGt                                                                                                                                            >  1:4856873/1‑150 (MQ=255)

         gCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAg                                                                                                                                          <  1:3155607/150‑1 (MQ=255)

            cGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGt                                                                                                                                       >  1:109901/1‑150 (MQ=255)

             gACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTa                                                                                                                                      >  2:1095967/1‑150 (MQ=255)

                  gtgtGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAag                                                                                                                                 >  1:1463012/1‑150 (MQ=255)

                   tgtgGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAaga                                                                                                                                <  2:1886644/150‑1 (MQ=255)

                   tgtgGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAaga                                                                                                                                <  2:718405/150‑1 (MQ=255)

                      gggCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAggg                                                                                                                             <  2:1682786/150‑1 (MQ=255)

                       ggCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGc                                                                                                                            >  1:1938107/1‑150 (MQ=255)

                       ggCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGc                                                                                                                            >  2:4976058/1‑150 (MQ=255)

                        gCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCg                                                                                                                           <  2:3624612/150‑1 (MQ=255)

                             aCCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTAc                                                                                                                      <  1:363922/150‑1 (MQ=255)

                               cGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCt                                                                                                                    <  2:1514194/150‑1 (MQ=255)

                               cGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCt                                                                                                                    >  1:3209446/1‑150 (MQ=255)

                                 aaCTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGa                                                                                                                  >  2:3777210/1‑150 (MQ=255)

                                  aCTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGAc                                                                                                                 <  2:3020766/150‑1 (MQ=255)

                                     gtgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGa                                                                                                              <  1:4418552/150‑1 (MQ=255)

                                     gtgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGa                                                                                                              <  1:4418171/150‑1 (MQ=255)

                                      tgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAg                                                                                                             <  2:4868122/150‑1 (MQ=255)

                                        cAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGACTCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATCAAGAGCGCGGAAACCTGACCGAGGc                                                                                                           >  2:3055954/1‑150 (MQ=255)

                                         aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                          >  1:4980261/1‑150 (MQ=255)

                                         aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                          <  2:3371042/150‑1 (MQ=255)

                                         aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                          <  2:535251/150‑1 (MQ=255)

                                              gcTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTgcg                                                                                                     <  1:314100/150‑1 (MQ=255)

                                                tGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTgcggc                                                                                                   >  1:1792238/1‑150 (MQ=255)

                                                  tacGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGTCTAGGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGgcgc                                                                                                 >  2:1720666/3‑150 (MQ=255)

                                                  gCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGTCTGATCGGTGCACCACCAGGCTAGGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGgcgc                                                                                                 >  1:1860933/1‑150 (MQ=255)

                                                      aGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGc                                                                                             <  2:5094289/150‑1 (MQ=255)

                                                        ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                           <  1:5071062/150‑1 (MQ=255)

                                                        ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                           <  2:2253484/150‑1 (MQ=255)

                                                        ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                           <  1:4203900/150‑1 (MQ=255)

                                                          ccTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTa                                                                                         <  1:606178/150‑1 (MQ=255)

                                                           cTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTAc                                                                                        >  2:1554488/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCTTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  2:2535266/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  2:4160058/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  2:4399039/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  2:4399040/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  1:1343606/1‑150 (MQ=255)

                                                            tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                       >  1:2265688/1‑150 (MQ=255)

                                                              ttCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCg                                                                                     >  1:3419804/1‑150 (MQ=255)

                                                               tCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCgg                                                                                    <  1:2925512/150‑1 (MQ=255)

                                                               tCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCgg                                                                                    >  1:4489591/1‑150 (MQ=255)

                                                                 gACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggtg                                                                                  <  2:2238200/150‑1 (MQ=255)

                                                                    acCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGgtggtg                                                                               >  2:139721/1‑150 (MQ=255)

                                                                       gAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgctg                                                                            <  1:626792/150‑1 (MQ=255)

                                                                         agagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTgctgct                                                                          <  1:2731434/150‑1 (MQ=255)

                                                                         agagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTgctgct                                                                          <  1:4859703/150‑1 (MQ=255)

                                                                          gagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGctgctg                                                                         >  2:3729258/1‑150 (MQ=255)

                                                                             gCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGAc                                                                      <  2:3794680/150‑1 (MQ=255)

                                                                             gCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGAc                                                                      >  1:3393983/1‑150 (MQ=255)

                                                                               gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                    >  1:4048457/1‑150 (MQ=255)

                                                                                 tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAgg                                                                  <  2:1723059/150‑1 (MQ=255)

                                                                                 tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAgg                                                                  <  2:573633/150‑1 (MQ=255)

                                                                                 tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAgg                                                                  <  1:3856775/150‑1 (MQ=255)

                                                                                   gTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTg                                                                <  2:3470866/150‑1 (MQ=255)

                                                                                    tGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTgg                                                               <  2:3183670/150‑1 (MQ=255)

                                                                                         aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                          <  1:3973117/150‑1 (MQ=255)

                                                                                         aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                          <  2:309690/150‑1 (MQ=255)

                                                                                          tCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAg                                                         <  2:4113092/150‑1 (MQ=255)

                                                                                             aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                      <  1:564603/150‑1 (MQ=255)

                                                                                                    cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                               >  1:5045291/1‑150 (MQ=255)

                                                                                                     gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                              >  1:2521010/1‑150 (MQ=255)

                                                                                                          cATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGtt                                         <  2:107105/150‑1 (MQ=255)

                                                                                                           aTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAGGCCCACCCGGATGTGTTc                                        >  2:116913/1‑150 (MQ=255)

                                                                                                              gagaAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAAc                                     >  2:262133/1‑150 (MQ=255)

                                                                                                                  aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                 <  2:1938107/150‑1 (MQ=255)

                                                                                                                   gCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCt                                <  2:4692978/150‑1 (MQ=255)

                                                                                                                      cTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGtt                             <  2:2638010/150‑1 (MQ=255)

                                                                                                                       tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                            >  1:2424574/1‑150 (MQ=255)

                                                                                                                       tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                            >  2:2187866/1‑150 (MQ=255)

                                                                                                                       tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                            >  2:2180639/1‑150 (MQ=255)

                                                                                                                       tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGAATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                            >  1:4994429/1‑150 (MQ=255)

                                                                                                                              gccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGttc                     <  2:3923675/145‑3 (MQ=255)

                                                                                                                                 gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                  <  2:487844/150‑1 (MQ=255)

                                                                                                                                  tcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGa                 <  2:2857147/149‑1 (MQ=255)

                                                                                                                                   cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                <  1:973065/150‑2 (MQ=255)

                                                                                                                                     gATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGa              >  1:3025363/1‑150 (MQ=255)

                                                                                                                                       tCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACg            <  2:4856873/150‑1 (MQ=255)

                                                                                                                                         ggTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgc          >  2:148460/1‑150 (MQ=255)

                                                                                                                                          gTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACggtc         >  2:987736/1‑148 (MQ=255)

                                                                                                                                          gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc         <  1:1955447/150‑1 (MQ=255)

                                                                                                                                          gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc         >  2:2042496/1‑150 (MQ=255)

                                                                                                                                            gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc       <  2:829080/150‑1 (MQ=255)

                                                                                                                                            gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc       <  2:3209446/150‑1 (MQ=255)

                                                                                                                                            gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc       <  1:255359/150‑1 (MQ=255)

                                                                                                                                            gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc       <  2:4337189/150‑1 (MQ=255)

                                                                                                                                             caccacCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgcc      >  2:708257/1‑150 (MQ=255)

                                                                                                                                              accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt     >  2:2226865/1‑150 (MQ=255)

                                                                                                                                                caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa   >  2:1567972/1‑150 (MQ=255)

                                                                                                                                                caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa   >  1:3386690/1‑150 (MQ=255)

                                                                                                                                                caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa   >  1:2918298/1‑150 (MQ=255)

                                                                                                                                                 accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc  <  1:2463938/150‑1 (MQ=255)

                                                                                                                                                 accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc  <  1:2622529/150‑1 (MQ=255)

TGTTCCTCGGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAC  >  NC_002947/732268‑732562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`