BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,455	G→A	10.0%	E664E (GAG→GAA)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,455	0	G	A	10.0%	506.9 / 34.8	179	E664E (GAG→GAA)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base G (89/72); new base A (4/14); total (93/86)
Fisher's exact test for biased strand distribution p-value = 1.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

TGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGA  >  NC_002947/732306‑732603

tgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAg                                                                                                                                                       <  2:4868122/150‑1 (MQ=255)

  cAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGACTCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATCAAGAGCGCGGAAACCTGACCGAGGc                                                                                                                                                     >  2:3055954/1‑150 (MQ=255)

   aaGGCGCTGGCCTAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCTCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                                                                    <  2:2198245/150‑1 (MQ=255)

   aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                                                                    <  1:4603538/150‑1 (MQ=255)

   aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                                                                    <  2:3371042/150‑1 (MQ=255)

   aaGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGcc                                                                                                                                                    <  2:1765679/150‑1 (MQ=255)

     ggCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGt                                                                                                                                                  >  1:4936704/1‑150 (MQ=255)

          tGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTgcggc                                                                                                                                             >  1:1792238/1‑150 (MQ=255)

            gCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGTCTGATCGGTGCACCACCAGGCTAGGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGgcgc                                                                                                                                           >  1:1860933/1‑150 (MQ=255)

            gCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGgcgc                                                                                                                                           >  2:1720542/1‑150 (MQ=255)

                aGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGc                                                                                                                                       <  2:5094289/150‑1 (MQ=255)

                  ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                                                                     <  1:4203900/150‑1 (MQ=255)

                  ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                                                                     >  2:923043/1‑150 (MQ=255)

                     cTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTAc                                                                                                                                  >  2:1554435/1‑150 (MQ=255)

                      tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCTTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                                                                 >  2:2535266/1‑150 (MQ=255)

                      tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                                                                 >  2:4160058/1‑150 (MQ=255)

                      tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                                                                 >  2:4399039/1‑150 (MQ=255)

                      tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                                                                 >  1:1343606/1‑150 (MQ=255)

                      tGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACt                                                                                                                                 >  1:2265688/1‑150 (MQ=255)

                        ttCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCg                                                                                                                               >  1:3419804/1‑150 (MQ=255)

                        ttCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAAATCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAATAGGGCGGTTACCTGACCGAGTCCGTGCGGCGCAAGCCTTACTCg                                                                                                                               >  1:3419683/1‑150 (MQ=255)

                         tCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCgg                                                                                                                              <  1:2925512/150‑1 (MQ=255)

                         tCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCgg                                                                                                                              >  1:4489591/1‑150 (MQ=255)

                           gACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggtg                                                                                                                            <  2:2238200/150‑1 (MQ=255)

                              acCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGgtggtg                                                                                                                         >  1:3091231/1‑150 (MQ=255)

                               ccGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgc                                                                                                                        >  2:3757244/1‑150 (MQ=255)

                                 gAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgctg                                                                                                                      <  1:626792/150‑1 (MQ=255)

                                   agagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTgctgct                                                                                                                    <  1:4859703/150‑1 (MQ=255)

                                     agGCGATGGTGCGCATCGACATGTCCGAAGTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTgg                                                                                                                  <  2:225991/150‑1 (MQ=255)

                                         gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                              >  1:4048457/1‑150 (MQ=255)

                                           tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAgg                                                                                                            <  2:1723059/150‑1 (MQ=255)

                                           tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAgg                                                                                                            <  1:3655168/150‑1 (MQ=255)

                                             gTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTg                                                                                                          <  2:3470866/150‑1 (MQ=255)

                                               gcgcATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGa                                                                                                        >  2:571949/1‑150 (MQ=255)

                                                   aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                    <  1:3973111/150‑1 (MQ=255)

                                                   aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                    <  2:309701/150‑1 (MQ=255)

                                                   aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                    <  2:309690/150‑1 (MQ=255)

                                                    tCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAg                                                                                                   <  2:4113092/150‑1 (MQ=255)

                                                       aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                                                                <  1:564603/150‑1 (MQ=255)

                                                            tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCGATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                                                           >  1:1511557/1‑150 (MQ=255)

                                                              cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                         >  1:5045291/1‑150 (MQ=255)

                                                              cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                         >  1:27707/1‑150 (MQ=255)

                                                               gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                        >  1:2521010/1‑150 (MQ=255)

                                                               gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                        >  1:2520963/1‑150 (MQ=255)

                                                                     aTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAGGCCCACCCGGATGTGTTc                                                                                  >  2:116913/1‑150 (MQ=255)

                                                                        gagaAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAAc                                                                               >  2:262133/1‑150 (MQ=255)

                                                                            aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                           <  2:1938107/150‑1 (MQ=255)

                                                                            aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                           <  2:1938059/150‑1 (MQ=255)

                                                                                 tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                      >  1:2424574/1‑150 (MQ=255)

                                                                                 tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                      >  2:2180639/1‑150 (MQ=255)

                                                                                 tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                      >  2:473241/1‑150 (MQ=255)

                                                                                 tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                      >  1:5057831/1‑150 (MQ=255)

                                                                                 tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGAATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                      >  1:4994429/1‑150 (MQ=255)

                                                                                      cgcgcgCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGt                                                                 <  2:2694433/150‑1 (MQ=255)

                                                                                        gccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGttc                                                               <  2:3923675/145‑3 (MQ=255)

                                                                                          cgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCtt                                                             <  2:1006418/147‑2 (MQ=255)

                                                                                           gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                            <  2:487844/150‑1 (MQ=255)

                                                                                           gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                            <  2:595353/150‑1 (MQ=255)

                                                                                             cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCGGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                          <  1:318518/150‑2 (MQ=255)

                                                                                             cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                                                          >  2:504895/1‑150 (MQ=255)

                                                                                               gATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTtgatga                                                        >  1:599450/1‑150 (MQ=255)

                                                                                               gATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGa                                                        >  1:3025363/1‑150 (MQ=255)

                                                                                                   ggTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgc                                                    >  2:148460/1‑150 (MQ=255)

                                                                                                    gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                   <  1:1955447/150‑1 (MQ=255)

                                                                                                    gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                   >  2:2042496/1‑150 (MQ=255)

                                                                                                      gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                 <  2:4337189/150‑1 (MQ=255)

                                                                                                      gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                 <  1:255359/150‑1 (MQ=255)

                                                                                                      gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                 <  2:3209446/150‑1 (MQ=255)

                                                                                                        accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                               >  2:2226865/1‑150 (MQ=255)

                                                                                                          caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                             >  2:786427/1‑150 (MQ=255)

                                                                                                          caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                             >  1:3386690/1‑150 (MQ=255)

                                                                                                          caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                             >  2:1567972/1‑150 (MQ=255)

                                                                                                           accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                            <  1:2622529/150‑1 (MQ=255)

                                                                                                             ccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCg                                          >  1:3994997/6‑150 (MQ=255)

                                                                                                                 cTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg                                      <  2:3839826/150‑1 (MQ=255)

                                                                                                                  tATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCAGATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggt                                     <  2:4564983/150‑4 (MQ=255)

                                                                                                                  tATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGc                                     >  2:4581149/1‑150 (MQ=255)

                                                                                                                        gggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg‑g                              <  2:21374/150‑1 (MQ=255)

                                                                                                                        gggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg‑g                              >  2:3700267/1‑150 (MQ=255)

                                                                                                                            aTGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCa                          >  1:912260/1‑150 (MQ=255)

                                                                                                                            aTGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCa                          >  1:912257/1‑150 (MQ=255)

                                                                                                                              gAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAcc                        >  2:3625296/1‑150 (MQ=255)

                                                                                                                              gAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAcc                        >  2:2506504/1‑150 (MQ=255)

                                                                                                                                 gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                     <  1:1720542/150‑1 (MQ=255)

                                                                                                                                 gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                     <  2:4489591/150‑1 (MQ=255)

                                                                                                                                 gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                     <  1:212319/150‑1 (MQ=255)

                                                                                                                                    gtcgtttACCTGACCGAGGCAGTGCGTCGCAAGCCTTTCTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACTGCCGCCTGACCGACAGCCTCGGTGCGC‑CCGTggct                  <  2:3419683/145‑3 (MQ=37)

                                                                                                                                        gcgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTcc              <  2:1928968/145‑1 (MQ=255)

                                                                                                                                        gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc              <  2:1860933/150‑1 (MQ=255)

                                                                                                                                         cgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCg             <  1:4710943/146‑1 (MQ=255)

                                                                                                                                           aCCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCa           >  2:2079319/1‑150 (MQ=255)

                                                                                                                                            ccTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCaa          >  1:4714913/1‑150 (MQ=255)

                                                                                                                                             cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac         >  2:1796831/1‑150 (MQ=255)

                                                                                                                                             cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac         >  1:3920785/1‑150 (MQ=255)

                                                                                                                                               gACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAacac       >  1:3154591/1‑150 (MQ=255)

                                                                                                                                                 ccGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcg     <  2:1463012/150‑1 (MQ=255)

                                                                                                                                                 ccGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcg     >  1:823569/1‑150 (MQ=255)

                                                                                                                                                  cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt    >  2:2905976/1‑150 (MQ=255)

                                                                                                                                                  cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt    <  2:1343606/150‑1 (MQ=255)

                                                                                                                                                    aGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtga  >  1:1129830/1‑150 (MQ=255)

TGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGA  >  NC_002947/732306‑732603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`