BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,489	C→T	12.7%	L676L (CTG→TTG) ‡	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,489	0	C	T	12.7%	410.5 / 41.4	157	L676L (CTG→TTG) ‡	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base C (75/62); new base T (5/15); total (80/77)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 165 total aligned reads displayed.

AGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATT  >  NC_002947/732341‑732638

agagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTgctgct                                                                                                                                                       <  1:2731434/150‑1 (MQ=255)

 gagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGctgctg                                                                                                                                                      >  2:3729258/1‑150 (MQ=255)

      gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                 >  1:1370649/1‑150 (MQ=255)

      gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                 >  2:2162069/1‑150 (MQ=255)

      gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                 <  1:3298093/150‑1 (MQ=255)

      gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                 >  1:4048457/1‑150 (MQ=255)

        tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAgg                                                                                                                                               <  1:3856775/150‑1 (MQ=255)

        tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAgg                                                                                                                                               <  1:3655168/150‑1 (MQ=255)

           tGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTgg                                                                                                                                            <  2:3183670/150‑1 (MQ=255)

            gcgcATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGa                                                                                                                                           >  2:571949/1‑150 (MQ=255)

                aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                                                       <  1:3973111/150‑1 (MQ=255)

                aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                                                       <  2:309690/150‑1 (MQ=255)

                 tCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAg                                                                                                                                      <  2:4113092/150‑1 (MQ=255)

                    aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                                                                                                   <  1:564603/150‑1 (MQ=255)

                        gTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGG‑AAAAGCCCACCCg                                                                                                                              >  2:1681878/1‑150 (MQ=255)

                         tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                                                                                              >  1:4327562/1‑150 (MQ=255)

                         tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCGATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                                                                                              >  1:1511557/1‑150 (MQ=255)

                           cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                            >  1:27707/1‑150 (MQ=255)

                           cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                            >  1:5045291/1‑150 (MQ=255)

                            gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                                                           >  1:2521010/1‑150 (MQ=255)

                            gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                                                           >  1:2520963/1‑150 (MQ=255)

                                 cATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGtt                                                                                                                      <  2:107105/150‑1 (MQ=255)

                                   tGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCa                                                                                                                    >  1:4872457/1‑150 (MQ=255)

                                         aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                                                              <  2:1938107/150‑1 (MQ=255)

                                         aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                                                              <  2:1938059/150‑1 (MQ=255)

                                             cTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGtt                                                                                                          <  2:2638010/150‑1 (MQ=255)

                                              tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                                                         >  2:473241/1‑150 (MQ=255)

                                              tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                                                         >  2:2180639/1‑150 (MQ=255)

                                              tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGAATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                                                         >  1:4994429/1‑150 (MQ=255)

                                                   cgcgcgCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGt                                                                                                    <  2:2694433/150‑1 (MQ=255)

                                                     gccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGttc                                                                                                  <  2:3923675/145‑3 (MQ=255)

                                                       cgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCtt                                                                                                <  2:1006418/147‑2 (MQ=255)

                                                        gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                               >  2:4397567/1‑150 (MQ=255)

                                                        gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                               <  2:487844/150‑1 (MQ=255)

                                                        gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                               <  2:595353/150‑1 (MQ=255)

                                                         tcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGa                                                                                              <  2:2857147/149‑1 (MQ=255)

                                                          cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                                                             <  1:2354814/150‑2 (MQ=255)

                                                          cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCGGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                                                             <  1:318518/150‑2 (MQ=255)

                                                                ggTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgc                                                                                       >  2:148586/1‑150 (MQ=255)

                                                                 gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                      <  1:1955447/150‑1 (MQ=255)

                                                                 gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                      >  2:2042496/1‑150 (MQ=255)

                                                                   gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                                                    <  2:4337168/150‑1 (MQ=255)

                                                                   gCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                                                    <  2:3209446/150‑1 (MQ=255)

                                                                     accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                                                                  >  2:2226865/1‑150 (MQ=255)

                                                                       caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                >  2:786427/1‑150 (MQ=255)

                                                                       caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                >  1:2918298/1‑150 (MQ=255)

                                                                       caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                >  1:3386690/1‑150 (MQ=255)

                                                                       caccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                >  2:1567972/1‑150 (MQ=255)

                                                                        accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                               <  1:2463938/150‑1 (MQ=255)

                                                                        accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                               <  1:2622529/150‑1 (MQ=255)

                                                                          ccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCg                                                                             >  1:3994997/6‑150 (MQ=255)

                                                                             gCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACa                                                                          <  1:4840670/150‑1 (MQ=255)

                                                                              cTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg                                                                         <  2:3839826/150‑1 (MQ=255)

                                                                               tATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCAGATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggt                                                                        <  2:4564983/150‑4 (MQ=255)

                                                                               tATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGc                                                                        >  2:4581149/1‑150 (MQ=255)

                                                                                 tGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggtca                                                                      >  2:2999406/1‑145 (MQ=255)

                                                                                 tGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggtca                                                                      <  1:3067712/150‑6 (MQ=255)

                                                                                    aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg                                                                   <  2:2527804/150‑1 (MQ=255)

                                                                                     gggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg‑g                                                                 >  2:3700267/1‑150 (MQ=255)

                                                                                         aTGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCa                                                             >  1:912260/1‑150 (MQ=255)

                                                                                           gAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAcc                                                           >  2:3625296/1‑150 (MQ=255)

                                                                                              gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                        <  1:212111/150‑1 (MQ=255)

                                                                                              gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                        <  1:1720542/150‑1 (MQ=255)

                                                                                                 gtcgtttACCTGACCGAGGCAGTGCGTCGCAAGCCTTTCTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACTGCCGCCTGACCGACAGCCTCGGTGCGC‑CCGTggct                                                     <  2:3419683/145‑3 (MQ=37)

                                                                                                 ggCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGTGCGC‑CCGTggct                                                     <  2:3419804/150‑3 (MQ=255)

                                                                                                     gcgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTcc                                                 <  2:1928968/145‑1 (MQ=255)

                                                                                                     gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                 <  2:1792238/150‑1 (MQ=255)

                                                                                                     gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                 <  2:1860933/150‑1 (MQ=255)

                                                                                                     gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                 <  2:1860862/150‑1 (MQ=255)

                                                                                                      cgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCg                                                <  1:4710728/146‑1 (MQ=255)

                                                                                                       gttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGc                                               <  1:3503442/147‑1 (MQ=255)

                                                                                                        aCCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCa                                              >  2:2079503/1‑150 (MQ=255)

                                                                                                        aCCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCa                                              >  2:2079319/1‑150 (MQ=255)

                                                                                                         ccTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCaa                                             >  1:4714913/1‑150 (MQ=255)

                                                                                                          cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac                                            >  1:259370/1‑150 (MQ=255)

                                                                                                          cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac                                            >  1:3920861/1‑150 (MQ=255)

                                                                                                            gACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAacac                                          >  1:896612/1‑150 (MQ=255)

                                                                                                            gACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAacac                                          >  1:3154591/1‑150 (MQ=255)

                                                                                                              ccGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcg                                        >  1:823569/1‑150 (MQ=255)

                                                                                                              ccGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcg                                        <  2:1463012/150‑1 (MQ=255)

                                                                                                               cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                       >  2:2345434/1‑150 (MQ=255)

                                                                                                                gAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtg                                      >  2:1247745/1‑150 (MQ=255)

                                                                                                                 aGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtga                                     >  1:1129830/1‑150 (MQ=255)

                                                                                                                       tGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTACTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtga                               <  2:4980261/150‑1 (MQ=255)

                                                                                                                        gcggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtgat                              <  1:4976058/150‑1 (MQ=255)

                                                                                                                           gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                            >  1:1553147/1‑54 (MQ=255)

                                                                                                                           gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                           >  2:823378/1‑150 (MQ=255)

                                                                                                                           gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                           >  2:33188/1‑150 (MQ=255)

                                                                                                                             gcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCt                         >  1:2698467/1‑150 (MQ=255)

                                                                                                                               aaGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTcc                       >  2:2274709/1‑150 (MQ=255)

                                                                                                                                 gCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCaa                     >  1:2271628/1‑150 (MQ=255)

                                                                                                                                  ccTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAc                    >  1:254600/1‑150 (MQ=255)

                                                                                                                                   cTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAcc                   >  2:3824508/1‑150 (MQ=255)

                                                                                                                                     tACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTg                 <  1:2162069/150‑1 (MQ=255)

                                                                                                                                      aCTCGGTGGTGCAGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTgg                <  2:1370649/150‑1 (MQ=255)

                                                                                                                                         aggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCTCGGcg             >  2:866040/2‑149 (MQ=255)

                                                                                                                                          ggtggtGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTc            <  2:4936704/150‑1 (MQ=255)

                                                                                                                                               tgctgcTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgcgc       <  1:571949/150‑1 (MQ=255)

                                                                                                                                                gctgctGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCa      <  2:5056906/150‑1 (MQ=255)

                                                                                                                                                gctgctGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCa      <  2:2971601/150‑1 (MQ=255)

AGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATT  >  NC_002947/732341‑732638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`