BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,494	C→T	12.0%	D677D (GAC→GAT)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,494	0	C	T	12.0%	446.8 / 41.0	167	D677D (GAC→GAT)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base C (77/70); new base T (5/15); total (82/85)
Fisher's exact test for biased strand distribution p-value = 3.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 169 total aligned reads displayed.

GCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGG  >  NC_002947/732345‑732642

gCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGAc                                                                                                                                                       >  1:3393983/1‑150 (MQ=255)

  gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                     >  1:1370649/1‑150 (MQ=255)

  gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                     >  2:2162069/1‑150 (MQ=255)

  gATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGa                                                                                                                                                     >  1:4048457/1‑150 (MQ=255)

    tGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAgg                                                                                                                                                   <  1:3856775/150‑1 (MQ=255)

      gTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTg                                                                                                                                                 <  2:3470866/150‑1 (MQ=255)

       tGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTgg                                                                                                                                                <  2:3183670/150‑1 (MQ=255)

        gcgcATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGa                                                                                                                                               >  2:571949/1‑150 (MQ=255)

            aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                                                           <  2:309690/150‑1 (MQ=255)

            aTCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaaa                                                                                                                                           <  1:3973111/150‑1 (MQ=255)

             tCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAg                                                                                                                                          <  2:4113092/150‑1 (MQ=255)

                aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                                                                                                       <  1:564603/150‑1 (MQ=255)

                    gTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGG‑AAAAGCCCACCCg                                                                                                                                  >  2:1681878/1‑150 (MQ=255)

                     tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                                                                                                  >  1:4327562/1‑150 (MQ=255)

                       cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                                >  2:3915543/1‑150 (MQ=255)

                       cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                                >  1:27707/1‑150 (MQ=255)

                        gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                                                               >  1:2520963/1‑150 (MQ=255)

                        gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                                                                                               >  1:2521010/1‑150 (MQ=255)

                             cATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGtt                                                                                                                          <  2:107105/150‑1 (MQ=255)

                                 gagaAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAAc                                                                                                                      >  2:262133/1‑150 (MQ=255)

                                     aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                                                                  <  2:1938107/150‑1 (MQ=255)

                                     aGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCAGCGCAAGCCGTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGc                                                                                                                  <  2:1938059/150‑1 (MQ=255)

                                      gCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCt                                                                                                                 <  2:4692978/150‑1 (MQ=255)

                                          tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                                                             >  1:5057831/1‑150 (MQ=255)

                                          tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                                                                                             >  2:2187866/1‑150 (MQ=255)

                                           ccGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGc                                                                                                            >  2:3694092/1‑150 (MQ=255)

                                               cgcgcgCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGt                                                                                                        <  2:2694433/150‑1 (MQ=255)

                                                 gccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGttc                                                                                                      <  2:3923675/145‑3 (MQ=255)

                                                  ccgacTGATTGGTGCACCTCCCGTATATGTCGGCTATGAACAGCGTCGCGTA‑CTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCt                                                                                                     <  2:3408224/146‑1 (MQ=255)

                                                    gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                                   <  2:595353/150‑1 (MQ=255)

                                                    gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                                   <  2:487844/150‑1 (MQ=255)

                                                     tcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGa                                                                                                  <  2:2857147/149‑1 (MQ=255)

                                                      cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                                                                 <  1:973065/150‑2 (MQ=255)

                                                      cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                                                                 <  1:2354814/150‑2 (MQ=255)

                                                      cTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCGGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGat                                                                                                 <  1:318518/150‑2 (MQ=255)

                                                      cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                                                                                                 >  2:504895/1‑150 (MQ=255)

                                                        gATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTtgatga                                                                                               >  1:599450/1‑150 (MQ=255)

                                                        gATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGa                                                                                               >  1:3025363/1‑150 (MQ=255)

                                                          tCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACg                                                                                             <  2:4856873/150‑1 (MQ=255)

                                                            ggTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgc                                                                                           >  2:148586/1‑150 (MQ=255)

                                                             gTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                          >  2:2042496/1‑150 (MQ=255)

                                                             gTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                          <  1:5041745/150‑1 (MQ=255)

                                                               gCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                                                        <  2:4337189/150‑1 (MQ=255)

                                                               gCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgc                                                                                        <  2:3209446/150‑1 (MQ=255)

                                                                caccacCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgccgcc                                                                                       >  2:708257/1‑150 (MQ=255)

                                                                 accaccAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                                                                      >  2:2226865/1‑150 (MQ=255)

                                                                   caccaGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                    >  2:4945908/1‑150 (MQ=255)

                                                                   caccaGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGa                                                                                    >  1:2918298/1‑150 (MQ=255)

                                                                    accaGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                                   <  1:2463938/150‑1 (MQ=255)

                                                                      ccggttATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCg                                                                                 >  1:3994997/6‑150 (MQ=255)

                                                                         gCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACa                                                                              <  1:4840670/150‑1 (MQ=255)

                                                                           tATGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCAGATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggt                                                                            <  2:4564983/150‑4 (MQ=255)

                                                                             tGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggtca                                                                          <  1:3067712/150‑6 (MQ=255)

                                                                             tGTCGGCTATGAAGAG‑GGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAcggtca                                                                          >  2:2999406/1‑145 (MQ=255)

                                                                                aGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg                                                                       <  2:2527804/150‑1 (MQ=255)

                                                                                 gggTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg‑g                                                                     >  2:3700267/1‑150 (MQ=255)

                                                                                    tATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑gcgc                                                                  >  1:3155617/1‑150 (MQ=255)

                                                                                     aTGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCa                                                                 >  1:912260/1‑150 (MQ=255)

                                                                                          gag‑GGCTGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                            <  1:1720666/150‑1 (MQ=255)

                                                                                          gag‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                            <  1:212111/150‑1 (MQ=255)

                                                                                          gag‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                            <  2:4489591/150‑1 (MQ=255)

                                                                                              gtcgtttACCTGACCGAGGCAGTGCGTCGCAAGCCTTTCTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACTGCCGCCTGACCGACAGCCTCGGTGCGC‑CCGTggct                                                         <  2:3419683/145‑3 (MQ=37)

                                                                                                  gcgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTcc                                                     <  2:1928968/145‑1 (MQ=255)

                                                                                                  gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                     <  2:1860933/150‑1 (MQ=255)

                                                                                                  gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                     <  2:1792238/150‑1 (MQ=255)

                                                                                                   cgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCg                                                    <  1:4710728/146‑1 (MQ=255)

                                                                                                   cgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCg                                                    <  1:4710943/146‑1 (MQ=255)

                                                                                                    gttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGc                                                   <  1:3503442/147‑1 (MQ=255)

                                                                                                     aCCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCa                                                  >  2:2079319/1‑150 (MQ=255)

                                                                                                     aCCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCa                                                  >  2:2079503/1‑150 (MQ=255)

                                                                                                       cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac                                                >  1:259370/1‑150 (MQ=255)

                                                                                                       cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac                                                >  1:3920861/1‑150 (MQ=255)

                                                                                                       cTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAac                                                >  2:1796831/1‑150 (MQ=255)

                                                                                                         gACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAacac                                              >  1:896612/1‑150 (MQ=255)

                                                                                                           ccGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcg                                            <  2:1463012/150‑1 (MQ=255)

                                                                                                            cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                           >  2:2905976/1‑150 (MQ=255)

                                                                                                            cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                           >  2:2345434/1‑150 (MQ=255)

                                                                                                            cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                           <  2:1343606/150‑1 (MQ=255)

                                                                                                            cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGATGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                           >  1:1576827/1‑150 (MQ=255)

                                                                                                             gAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtg                                          >  2:1247745/1‑150 (MQ=255)

                                                                                                              aGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtga                                         >  1:1129830/1‑150 (MQ=255)

                                                                                                                gCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatc                                       <  1:1471410/150‑1 (MQ=255)

                                                                                                                     gcggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtgat                                  <  1:4976058/150‑1 (MQ=255)

                                                                                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                                <  2:1553147/54‑1 (MQ=255)

                                                                                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                                                                                                >  1:1553147/1‑54 (MQ=255)

                                                                                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                               >  2:33188/1‑150 (MQ=255)

                                                                                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                               >  2:823378/1‑150 (MQ=255)

                                                                                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                               <  1:33188/150‑1 (MQ=255)

                                                                                                                          gcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCt                             >  1:2698467/1‑150 (MQ=255)

                                                                                                                          gcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCt                             >  1:2698313/1‑150 (MQ=255)

                                                                                                                              gCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCaa                         >  1:2271628/1‑150 (MQ=255)

                                                                                                                               ccTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAc                        >  1:254600/1‑150 (MQ=255)

                                                                                                                                cTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAcc                       >  2:3824508/1‑150 (MQ=255)

                                                                                                                                  tACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTg                     <  1:2162069/150‑1 (MQ=255)

                                                                                                                                      aggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCTCGGcg                 >  2:866040/2‑149 (MQ=255)

                                                                                                                                                 ctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGAtt      >  1:4911611/1‑150 (MQ=255)

                                                                                                                                                  tgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTc     <  1:4399040/150‑1 (MQ=255)

                                                                                                                                                   ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa    <  2:5045291/150‑1 (MQ=255)

                                                                                                                                                    gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg   <  1:1963749/150‑1 (MQ=255)

                                                                                                                                                    gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg   >  1:3776849/1‑150 (MQ=255)

GCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAG‑GGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGG  >  NC_002947/732345‑732642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`