BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	988,875	A→G	5.8%	K242E (AAG→GAG)	hisS →	histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	988,875	0	A	G	5.8%	596.5 / 16.0	188	K242E (AAG→GAG)	hisS	histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (96/81); new base G (7/4); total (103/85)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

Only 100 of 189 total aligned reads displayed.

TGGACGAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGTTACGACGGC  >  NC_002947/988729‑989024

tGGACGAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAgg                                                                                                                                                    >  2:2053739/1‑150 (MQ=255)

 ggACGAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGc                                                                                                                                                   <  2:1812646/150‑1 (MQ=255)

    cGAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTTGAGGccct                                                                                                                                                <  1:3287763/150‑2 (MQ=255)

     gAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGc                                                                                                                                               >  2:5113011/1‑150 (MQ=255)

     gAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGc                                                                                                                                               >  1:1253967/1‑150 (MQ=255)

     gAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGc                                                                                                                                               >  1:868132/1‑150 (MQ=255)

        gACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTTGAGGccctcgaa                                                                                                                                            <  2:4800431/150‑6 (MQ=255)

        gACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTTGAGGccctcgaa                                                                                                                                            <  2:3477052/150‑6 (MQ=255)

        gACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTg                                                                                                                                            >  2:1515286/1‑150 (MQ=255)

                gcgcCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGc                                                                                                                                    <  1:1403349/150‑1 (MQ=255)

                  gccgccTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCg                                                                                                                                  <  1:3340318/150‑1 (MQ=255)

                    cgccTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGt                                                                                                                                <  1:1794817/150‑1 (MQ=255)

                       cTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTAtt                                                                                                                             >  1:1352962/1‑150 (MQ=255)

                        tGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTc                                                                                                                            >  1:4254895/1‑150 (MQ=255)

                            gagCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGtt                                                                                                                        <  1:749212/150‑1 (MQ=255)

                             agCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTc                                                                                                                       >  1:3935991/1‑150 (MQ=255)

                               cAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGt                                                                                                                     >  2:955467/1‑150 (MQ=255)

                               cAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGt                                                                                                                     <  2:1669935/150‑1 (MQ=255)

                               cAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGt                                                                                                                     >  2:3682279/1‑150 (MQ=255)

                                aaCCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTg                                                                                                                    >  1:570559/1‑150 (MQ=255)

                                aaCCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTg                                                                                                                    <  2:410844/150‑1 (MQ=255)

                                 aCCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGa                                                                                                                   <  1:105643/150‑1 (MQ=255)

                                  cccGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGACGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGAt                                                                                                                  >  2:2593910/1‑150 (MQ=255)

                                  cccGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGAt                                                                                                                  <  2:1632018/150‑1 (MQ=255)

                                     gCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCaa                                                                                                               <  1:3416645/150‑1 (MQ=255)

                                         cgcATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACAcc                                                                                                           >  2:1855737/1‑150 (MQ=255)

                                           cATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCaa                                                                                                         >  1:193046/1‑150 (MQ=255)

                                                tCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTgg                                                                                                    >  2:4074505/1‑150 (MQ=255)

                                                       cAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGccc                                                                                                                                                 <  1:1808144/98‑1 (MQ=255)

                                                       cAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCgg                                                                                             >  1:2746906/1‑150 (MQ=255)

                                                       cAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCACGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCgg                                                                                             >  2:2685265/1‑150 (MQ=255)

                                                         aGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGcc                                                                                           >  2:3039586/1‑150 (MQ=255)

                                                           gACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTg                                                                                         <  2:3997442/150‑1 (MQ=255)

                                                           gACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTg                                                                                         >  1:2390067/1‑150 (MQ=255)

                                                             ccAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGa                                                                                       <  1:2207805/150‑1 (MQ=255)

                                                             ccAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGa                                                                                       <  2:4995315/150‑1 (MQ=255)

                                                             ccAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGa                                                                                       <  2:3643527/150‑1 (MQ=255)

                                                              cAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGac                                                                                      >  2:39573/1‑150 (MQ=255)

                                                               aGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGact                                                                                     >  1:836722/1‑150 (MQ=255)

                                                               aGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGact                                                                                     >  1:48160/1‑150 (MQ=255)

                                                               aGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGact                                                                                     >  2:2786402/1‑150 (MQ=255)

                                                                gAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGacta                                                                                    <  2:2635091/150‑1 (MQ=255)

                                                                   cacCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGActacta                                                                                 <  2:833109/150‑1 (MQ=255)

                                                                   cacCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGActacta                                                                                 <  2:2810647/150‑1 (MQ=255)

                                                                    acCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACtactac                                                                                <  1:3744288/150‑1 (MQ=255)

                                                                     cccAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACa                                                                               <  2:675154/150‑1 (MQ=255)

                                                                      ccAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAg                                                                              >  2:4617411/1‑150 (MQ=255)

                                                                      ccAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTAGTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTCCAg                                                                              >  2:4589172/1‑150 (MQ=255)

                                                                       cAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGc                                                                             >  1:3020100/1‑150 (MQ=255)

                                                                       cAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGc                                                                             <  1:4096343/150‑1 (MQ=255)

                                                                          gCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAg                                                                          <  1:4345801/150‑1 (MQ=255)

                                                                          gCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAg                                                                          >  1:1697404/1‑150 (MQ=255)

                                                                          gCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAg                                                                          <  1:1017329/150‑1 (MQ=255)

                                                                             gTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGAACTACTACAGCAAGAcc                                                                       >  1:796657/1‑150 (MQ=255)

                                                                               gCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCgt                                                                     >  1:4901518/1‑150 (MQ=255)

                                                                                 tGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCAGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCgtgt                                                                   >  1:2107734/1‑150 (MQ=255)

                                                                                  ggTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGtt                                                                  <  2:906248/150‑1 (MQ=255)

                                                                                   gTCGGAGCGCCGAAGCTGGAAGACTACCTAGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTc                                                                 >  2:3944956/1‑150 (MQ=255)

                                                                                       gAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGt                                                             <  2:1597552/150‑1 (MQ=255)

                                                                                       gAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGt                                                             <  1:4225307/150‑1 (MQ=255)

                                                                                       gAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGt                                                             <  2:308830/150‑1 (MQ=255)

                                                                                        aGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTg                                                            >  1:3354289/1‑150 (MQ=255)

                                                                                         gcgcCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTgg                                                           <  2:193046/150‑1 (MQ=255)

                                                                                         gcgcCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTgg                                                           <  1:4383146/150‑1 (MQ=255)

                                                                                            ccGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTa                                                        >  1:1176613/1‑150 (MQ=255)

                                                                                                aGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCg                                                    >  2:1015906/1‑150 (MQ=255)

                                                                                                    ggAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACaa                                                <  1:4199260/150‑1 (MQ=255)

                                                                                                    ggAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACaa                                                <  1:1765546/150‑1 (MQ=255)

                                                                                                      aaGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGc                                              >  1:3457633/1‑150 (MQ=255)

                                                                                                              cTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGcc                                      >  2:2564867/1‑150 (MQ=255)

                                                                                                               tGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTAGTCCGTTCATGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGccc                                     <  2:4749854/150‑1 (MQ=255)

                                                                                                                ggACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCa                                    <  2:3358882/150‑1 (MQ=255)

                                                                                                                   cGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAggg                                 <  1:69591/150‑1 (MQ=255)

                                                                                                                    gaagaaTCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGc                                >  1:1981722/1‑150 (MQ=255)

                                                                                                                    gaagaaTCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGc                                >  1:891667/1‑150 (MQ=255)

                                                                                                                    gaagaaTCGCGCGTGCACTTCGAGGACCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGc                                >  1:1449389/1‑150 (MQ=255)

                                                                                                                       gaaTCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACg                             <  2:3288495/150‑1 (MQ=255)

                                                                                                                           cgcgcgTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGgtgt                         <  2:4341326/150‑1 (MQ=255)

                                                                                                                            gcgcgTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAgg                                  >  1:4714572/1‑140 (MQ=255)

                                                                                                                            gcgcgTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAgg                                  <  2:4714572/140‑1 (MQ=255)

                                                                                                                               cgTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTgcgc                     <  2:1132127/150‑1 (MQ=255)

                                                                                                                                 tGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCTAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCgg                   <  1:3046866/150‑1 (MQ=255)

                                                                                                                                 tGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCgg                   <  1:1515286/150‑1 (MQ=255)

                                                                                                                                 tGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCgg                   <  2:570559/150‑1 (MQ=255)

                                                                                                                                 tGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCgg                   <  1:609760/150‑1 (MQ=255)

                                                                                                                                  gCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCggg                  <  1:3944956/150‑1 (MQ=255)

                                                                                                                                     cTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTgg               >  2:3832842/1‑150 (MQ=255)

                                                                                                                                     cTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTgg               <  1:2579358/150‑1 (MQ=255)

                                                                                                                                      ttCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGc              >  2:3707083/1‑150 (MQ=255)

                                                                                                                                      ttCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGc              >  2:4304956/1‑150 (MQ=255)

                                                                                                                                       tCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGcc             <  2:1253967/150‑1 (MQ=255)

                                                                                                                                       aggcgggCCTTGAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGcc             >  1:2393175/5‑150 (MQ=255)

                                                                                                                                       aggcgggCCTTGAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGcc             >  1:2393195/5‑150 (MQ=255)

                                                                                                                                         gcgggCCTTGAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGt           >  1:1979135/3‑150 (MQ=255)

                                                                                                                                         gAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGt           <  2:2746925/150‑1 (MQ=255)

                                                                                                                                          aGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGtt          <  2:2744373/150‑1 (MQ=255)

                                                                                                                                           gggCCTTGAGGCCCTCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGTTa         >  2:297079/1‑150 (MQ=255)

                                                                                                                                           gggCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGTTa         <  2:2175190/150‑1 (MQ=255)

                                                                                                                                               cTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGTTacgac     >  1:4930871/1‑150 (MQ=255)

                                                                                                                                                tCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCgg                                          <  2:2452885/112‑1 (MQ=255)

TGGACGAAGACAGCCAGCGCCGCCTGAAGAGCAACCCGCTGCGCATCCTCGACAGCAAGGACCAGAACACCCAGGCGGTGCTGGTCGGAGCGCCGAAGCTGGAAGACTACCTGGACGAAGAATCGCGCGTGCACTTCGAGGGCCTCAAGGCCCGCCTGGACGCTGCCGGTATTCCGTTCGTGATCAACACCAAGCTGGTACGCGGCCTGGACTACTACAGCAAGACCGTGTTCGAGTGGGTAACCGACAAGCTCGGTGCCCAGGGCACGGTGTGCGCGGGTGGCCGTTACGACGGC  >  NC_002947/988729‑989024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`