Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,103,281 T→G 5.6% L12R (CTT→CGT)  murA → UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,103,2810TG 5.6% 612.4 / 18.7 190L12R (CTT→CGT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (78/100);  new base G (7/4);  total (86/104)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 194 total aligned reads displayed.

ATGCTCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCATGGGCA  >  NC_002947/1103132‑1103430
                                                                                                                                                     |                                                                                                                                                     
aTGCTCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCt                                                                                                                                                       >  1:3850853/1‑150 (MQ=255)
 tGCTCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCtt                                                                                                                                                      >  1:3341323/1‑150 (MQ=255)
   cTCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGa                                                                                                                                                    <  2:274184/150‑1 (MQ=255)
    tCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGAc                                                                                                                                                   >  2:4047085/1‑150 (MQ=255)
    tCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGAc                                                                                                                                                   >  2:528446/1‑150 (MQ=255)
       ccTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGc                                                                                                                                                <  1:482591/150‑1 (MQ=255)
       ccTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGc                                                                                                                                                <  2:4639445/150‑1 (MQ=255)
        cTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCg                                                                                                                                               >  2:4628288/1‑150 (MQ=255)
        cTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCg                                                                                                                                               >  2:321266/1‑150 (MQ=255)
             tCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATc                                                                                                                                          <  1:4955341/150‑1 (MQ=255)
             tCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATc                                                                                                                                          <  1:376937/150‑1 (MQ=255)
              ccGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCGTCAAGGCgagcgcc                                                                                                                                         <  1:2092296/150‑5 (MQ=255)
                gTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGc                                                                                                                                       <  1:1081898/150‑1 (MQ=255)
                  ggATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGATCTCGCCGTCAAGGCGAGcgccgcca                                                                                                                                     <  1:520854/150‑3 (MQ=255)
                     tCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTc                                                                                                                                  >  1:2827802/1‑150 (MQ=255)
                       gCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCgg                                                                                                                                <  2:3566789/150‑1 (MQ=255)
                       gCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCgg                                                                                                                                <  2:708262/150‑1 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               >  2:1839323/1‑150 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               >  2:3827677/1‑150 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               >  2:4884047/1‑150 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               >  1:1321377/1‑150 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               >  2:3460742/1‑150 (MQ=255)
                        ccAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCggg                                                                                                                               <  2:1234736/150‑1 (MQ=255)
                           aTGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGgcgc                                                                                                                            <  2:2994269/150‑1 (MQ=255)
                               cagcaTCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGaaa                                                                                                                        >  2:3031361/1‑150 (MQ=255)
                                 gcaTCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGaaaaa                                                                                                                      <  2:3784942/150‑1 (MQ=255)
                                 gcaTCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGaaaaa                                                                                                                      >  2:2011683/1‑150 (MQ=255)
                                     ccATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  <  2:1490177/150‑1 (MQ=255)
                                     ccATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  <  1:1351178/150‑1 (MQ=255)
                                     ccATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  >  2:2950249/1‑150 (MQ=255)
                                     ccATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  <  1:1555188/150‑1 (MQ=255)
                                     ccATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  <  2:2203817/150‑1 (MQ=255)
                                      cATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAg                                                                                                                 <  1:1589317/150‑1 (MQ=255)
                                       aTGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGc                                                                                                                <  2:4110952/150‑1 (MQ=255)
                                               aCCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGa                                                                                                        >  1:2395992/1‑150 (MQ=255)
                                               aCCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGa                                                                                                        >  1:2396392/1‑150 (MQ=255)
                                                 cATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGAtt                                                                                                      >  2:3865627/1‑150 (MQ=255)
                                                 cATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGAtt                                                                                                      <  2:3064651/150‑1 (MQ=255)
                                                   tGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCACTGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCt                                                                                                    <  2:4328059/150‑1 (MQ=255)
                                                   tGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCa                                                                                                                  <  1:3871754/136‑1 (MQ=255)
                                                   tGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCt                                                                                                    <  1:146586/150‑1 (MQ=255)
                                                   tGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCt                                                                                                    >  1:4782091/1‑150 (MQ=255)
                                                   tGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCt                                                                                                    <  2:4328042/150‑1 (MQ=255)
                                                       aTTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCa                                                                                                <  2:1597431/150‑1 (MQ=255)
                                                        tttttCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAg                                                                                               <  1:3938128/150‑1 (MQ=255)
                                                         ttttCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGc                                                                                              <  1:438379/150‑1 (MQ=255)
                                                         atttCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTCGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGc                                                                                              <  2:3893510/149‑1 (MQ=255)
                                                          tttCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGcc                                                                                             >  1:1135870/1‑150 (MQ=255)
                                                               gcagcgcagcCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCct                                                                                        >  1:2175642/1‑150 (MQ=255)
                                                                    gcagcCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCCCCCTGCTgg                                                                                   >  1:1894337/1‑150 (MQ=255)
                                                                        ccTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGa                                                                               <  1:3959842/150‑1 (MQ=255)
                                                                         cTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGAt                                                                              <  2:1321377/150‑1 (MQ=255)
                                                                          tGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATg                                                                             >  1:2013653/1‑150 (MQ=255)
                                                                          tGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATg                                                                             <  1:2955581/150‑1 (MQ=255)
                                                                             gCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGcc                                                                          >  1:4234454/1‑150 (MQ=255)
                                                                                gAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCgg                                                                       >  2:4179215/1‑150 (MQ=255)
                                                                                       ccTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGt                                                                <  1:3588531/150‑1 (MQ=255)
                                                                                         tGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTgg                                                              <  2:4867945/150‑1 (MQ=255)
                                                                                         tGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTgg                                                              <  2:4915917/150‑1 (MQ=255)
                                                                                         tGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTgg                                                              <  1:2746624/150‑1 (MQ=255)
                                                                                         tGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTgg                                                              <  2:3251487/150‑1 (MQ=255)
                                                                                         tGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATGACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTgg                                                              <  2:1018033/150‑1 (MQ=255)
                                                                                           aGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGc                                                            >  2:1660372/1‑150 (MQ=255)
                                                                                              cAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAAc                                                         >  2:3945510/1‑150 (MQ=255)
                                                                                                     gcggcgAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGc                                                  <  1:1228582/150‑1 (MQ=255)
                                                                                                        gcgAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGc                                                     <  2:4679343/144‑1 (MQ=255)
                                                                                                        gcgAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCAcc                                               <  1:4563762/150‑1 (MQ=255)
                                                                                                             tcTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCAc                                          <  2:4665063/149‑1 (MQ=255)
                                                                                                             aCTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCAc                                          <  2:1598507/150‑1 (MQ=255)
                                                                                                                ccAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGAc                                       <  1:1710535/150‑1 (MQ=255)
                                                                                                                  aaTGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACAt                                     <  2:4133036/150‑1 (MQ=255)
                                                                                                                  aaTGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACAt                                     <  2:4132815/150‑1 (MQ=255)
                                                                                                                     ggACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAATCGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATcac                                  >  2:2049010/1‑150 (MQ=255)
                                                                                                                       aCAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATcacca                                <  2:3096647/150‑1 (MQ=255)
                                                                                                                         aaaCTGATTATTACTGGCGGCGCTCGCCTTGACCGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCaccacc                              >  1:5036446/1‑150 (MQ=255)
                                                                                                                         aaaCTGATTATTACTGGCGGCGCTCGCCATGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCAGCTGGCCCATGGCCCGGTCACCCTGGGCAACCAGCCGCACCAGCACGACATCaccacc                              >  1:4025070/1‑150 (MQ=255)
                                                                                                                          aaCTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCAccacca                             <  2:1177611/150‑1 (MQ=255)
                                                                                                                           aCTGATTATTACTGGCGGCGCTCGCCTTTACGGCGAGATCCCCATTTAGGGCGCGAAAAAAGCAGCCCTGCCCATTATGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCAccaccaa                            >  1:3006558/1‑149 (MQ=255)
                                                                                                                           aCTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCAt                            >  1:2433837/1‑150 (MQ=255)
                                                                                                                             tGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGa                          <  2:3341413/150‑1 (MQ=255)
                                                                                                                             tGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGa                          >  2:3155138/1‑150 (MQ=255)
                                                                                                                             tGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGa                          >  2:4741488/1‑150 (MQ=255)
                                                                                                                               attattACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTTGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATc                        >  1:3191253/1‑150 (MQ=255)
                                                                                                                               attattACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATc                        <  2:3706830/150‑1 (MQ=255)
                                                                                                                                 tattaCTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGa                      <  1:803037/150‑1 (MQ=255)
                                                                                                                                 tattaCTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGa                      <  2:2444156/150‑1 (MQ=255)
                                                                                                                                       tGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGGTCGAGCTGtg                >  1:4169137/1‑149 (MQ=255)
                                                                                                                                        ggcggcGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTc               <  1:733380/150‑1 (MQ=255)
                                                                                                                                         gcggcgCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCg              >  1:3416340/1‑150 (MQ=255)
                                                                                                                                          cggatcTCGCCGTCAAGGCGAGCGCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCgg             >  2:2456012/6‑150 (MQ=255)
                                                                                                                                           ggatcTCGCCGTCAAGGCGAGCGCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCggg            >  1:233220/5‑150 (MQ=255)
                                                                                                                                           ggatcTCGCCGTCAAGGCGAGCGCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCggg            >  1:232888/5‑150 (MQ=255)
                                                                                                                                           ggatcTCGCCGTCAAGGCGAGCGCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCggg            >  1:3083774/5‑150 (MQ=255)
                                                                                                                                           ggaacTCGCCGTCAAGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCggg            >  1:1884836/5‑150 (MQ=255)
                                                                                                                                              tcTCGCCGTCAAGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGgcgc         >  2:4243435/2‑150 (MQ=255)
                                                                                                                                                tCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGTAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCAt       <  2:2651789/150‑1 (MQ=255)
                                                                                                                                                tCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCAt       >  2:2110341/1‑150 (MQ=255)
                                                                                                                                                  gCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCATgg     >  2:4432567/1‑150 (MQ=255)
                                                                                                                                                  gCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCATgg     >  2:3471172/1‑150 (MQ=255)
                                                                                                                                                    cTTGACGGCGAGATCCGCATTTCGGGCGCGAAAATCGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCATGGGc   >  2:2557394/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCTCACCTGAATCCGTGGATCGCCAATGGCAGCATCCATGCGGTAACCATGAAATTTTTCAGCAGCGCAGCCTGGGCTGAGCGCACCTGAGCCAACTTGGCGGCGAGACTCCAATGGACAAACTGATTATTACTGGCGGCGCTCGCCTTGACGGCGAGATCCGCATTTCGGGCGCGAAAAACGCAGCCCTGCCGATTCTGGCAGCCACCCTGCTGGCCGATGGCCCGGTCACCGTGGGCAACCTGCCGCACCTGCACGACATCACCACCATGATCGAGCTGTTCGGGCGCATGGGCA  >  NC_002947/1103132‑1103430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑