Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,148,802 A→C 7.7% L150R (CTT→CGT)  PP_1007 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,148,8020AC 7.7% 478.2 / 22.4 170L150R (CTT→CGT) PP_1007transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (81/73);  new base C (6/8);  total (87/83)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 176 total aligned reads displayed.

CCTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCTT  >  NC_002947/1148653‑1148950
                                                                                                                                                     |                                                                                                                                                    
ccTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTaa                                                                                                                                                      >  2:3053562/1‑150 (MQ=255)
ccTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTaa                                                                                                                                                      <  1:3285100/150‑1 (MQ=255)
ccTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTaa                                                                                                                                                      <  2:2649855/150‑1 (MQ=255)
ccTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTaa                                                                                                                                                      <  2:1974635/150‑1 (MQ=255)
ccTCGGGGACCTGGAAGTAAGCCACGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTaa                                                                                                                                                      <  2:4471603/150‑1 (MQ=255)
 cTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAg                                                                                                                                                     <  1:2041632/150‑1 (MQ=255)
    ggggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATTCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtg                                                                                                                                                  <  2:4425197/150‑1 (MQ=255)
    ggggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtg                                                                                                                                                  <  1:322271/150‑1 (MQ=255)
     gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtgg                                                                                                                                                 >  2:410342/1‑150 (MQ=255)
     gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtgg                                                                                                                                                 <  2:2794048/150‑1 (MQ=255)
       gACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTc                                                                                                                                               <  1:4626086/150‑1 (MQ=255)
        aCCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCg                                                                                                                                              <  2:4344972/150‑1 (MQ=255)
        aCCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTACAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTTGTCg                                                                                                                                              <  2:716270/150‑1 (MQ=255)
          cTGGAAGTAAGCCTCGCCCTGTAGCTGGGGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGATTGCCGATTTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGc                                                                                                                                            <  1:4838464/150‑1 (MQ=255)
          cTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGc                                                                                                                                            >  1:4523360/1‑150 (MQ=255)
          cTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGc                                                                                                                                            <  1:1509794/150‑1 (MQ=255)
            gtaaGTAAGCCTCGCCCTGTAGCTGGCGGGCGACGTGGCGGCCGTCCTGTCTTTCGCTGGCTAATGCCGAATTGTTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTAAGGTGGTCGGCCt                                                                                                                                          <  1:4019884/148‑1 (MQ=255)
              aaGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGc                                                                                                                                        >  2:4037423/1‑150 (MQ=255)
                gTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAg                                                                                                                                      <  2:306081/150‑1 (MQ=255)
                     ccTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCa                                                                                                                                 >  2:1126436/1‑150 (MQ=255)
                          cccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGc                                                                                                                            >  2:15750/1‑150 (MQ=255)
                           ccTGTAGCAGGCGGGCGCCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAAGGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCa                                                                                                                           <  1:1089068/150‑1 (MQ=255)
                           ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGACTGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCa                                                                                                                           >  1:3782871/1‑150 (MQ=255)
                           ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCa                                                                                                                           <  1:3155482/150‑1 (MQ=255)
                            cTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                          <  1:2741953/150‑1 (MQ=255)
                            cTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                          <  1:2741952/150‑1 (MQ=255)
                            cTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAg                                                                                                                          >  2:2531720/1‑150 (MQ=255)
                               tAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGtt                                                                                                                       >  2:4051035/1‑150 (MQ=255)
                                    ggcgggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGa                                                                                                                  <  2:1614441/150‑1 (MQ=255)
                                     gcgggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGAc                                                                                                                 >  2:2500006/1‑150 (MQ=255)
                                       gggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCCCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACgg                                                                                                               <  1:1814692/150‑1 (MQ=255)
                                        ggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCCGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGt                                                                                                              <  1:1911187/150‑1 (MQ=255)
                                         gcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTg                                                                                                             <  2:2464792/150‑1 (MQ=255)
                                            cccgggcggcCGGCCTGGCGTTCGGTGGCGAATGCGGATTGGGTGTTGAGCAGCACTTTGGCGCCGTCTTCCAGTGGCCGGCGCTGGCGCTCGCCGACCACGGTACGGGGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAAt                                                                                                          <  1:4298284/146‑1 (MQ=255)
                                               tGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGc                                                                                                       >  1:1542802/1‑150 (MQ=255)
                                                ggcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGcc                                                                                                      <  2:3823601/150‑1 (MQ=255)
                                                 gcggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCCGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGccc                                                                                                     <  2:4210862/150‑1 (MQ=255)
                                                   ggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGACTAGCCCGa                                                                                                   >  2:3670205/1‑150 (MQ=255)
                                                   ggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGa                                                                                                   >  2:2093358/1‑150 (MQ=255)
                                                    gcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGCCCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGAc                                                                                                  <  2:1354461/150‑1 (MQ=255)
                                                      cGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACca                                                                                                <  2:2253677/150‑1 (MQ=255)
                                                           tGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTCGGcgagc                                                                                                                                        >  2:2897833/1‑101 (MQ=255)
                                                           tGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTCGGcgagc                                                                                                                                        <  1:2897833/105‑5 (MQ=255)
                                                               gTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAgcg                                                                                       >  2:4113809/1‑150 (MQ=255)
                                                                    cTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCg                                                                                  >  2:1844429/1‑150 (MQ=255)
                                                                     tGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATa                                                                                                         <  2:3174656/126‑1 (MQ=255)
                                                                     tGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCgg                                                                                 >  2:2108188/1‑150 (MQ=255)
                                                                       gCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTg                                                                               >  2:2354726/1‑150 (MQ=255)
                                                                         gAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGc                                                                             >  1:397915/1‑150 (MQ=255)
                                                                         gAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCGGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGc                                                                             <  1:4016550/150‑1 (MQ=255)
                                                                            tGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAg                                                                          >  2:2839517/1‑150 (MQ=255)
                                                                              ccGATTTGGTGTTGAGCAGCACTTTGGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCCCCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                        <  1:3946334/150‑1 (MQ=255)
                                                                              ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                        <  2:2740174/150‑1 (MQ=255)
                                                                              ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTCCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                        <  1:350705/150‑1 (MQ=255)
                                                                               cGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAgggg                                                                       >  2:2781594/1‑150 (MQ=255)
                                                                                gAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGt                                                                                                              <  2:4564172/110‑1 (MQ=255)
                                                                                gAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGt                                                                      >  1:4979880/1‑150 (MQ=255)
                                                                                     ggTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCa                                                                 <  2:4979880/150‑1 (MQ=255)
                                                                                             gcagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgc                                                         >  2:4338444/1‑150 (MQ=255)
                                                                                               agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcgc                                                       >  1:601934/1‑150 (MQ=255)
                                                                                               agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcgc                                                       >  2:4166766/1‑150 (MQ=255)
                                                                                                gcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCa                                                      >  1:4158983/1‑150 (MQ=255)
                                                                                                    ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                                                  >  2:4152580/1‑150 (MQ=255)
                                                                                                    ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                                                  >  1:909800/1‑150 (MQ=255)
                                                                                                     tttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcgc                                                 <  1:4426935/150‑1 (MQ=255)
                                                                                                     tttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcgc                                                 <  1:1146733/150‑1 (MQ=255)
                                                                                                           ccGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCgcgc                                           <  2:3712873/150‑1 (MQ=255)
                                                                                                           ccGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCgcgc                                           <  1:425012/150‑1 (MQ=255)
                                                                                                           ccGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCgcgc                                           <  2:500649/150‑1 (MQ=255)
                                                                                                            cGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGcc                                          >  1:1214310/1‑150 (MQ=255)
                                                                                                             gTCTTCCAGTTGCTGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCa                                         <  1:258065/150‑1 (MQ=255)
                                                                                                             gTCTTCCAGTGGCAGGCGCGGGCGCTCGCCGACCCCCGTCAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCa                                         <  1:258047/150‑1 (MQ=255)
                                                                                                              tCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCAt                                        <  2:909800/150‑1 (MQ=255)
                                                                                                              tCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCAt                                        <  2:1723196/150‑1 (MQ=255)
                                                                                                              ccTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCAt                                        <  2:1723240/149‑1 (MQ=255)
                                                                                                                ttCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGa                                      >  2:3529557/1‑150 (MQ=255)
                                                                                                                  ccAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGAcc                                    >  2:5078884/1‑150 (MQ=255)
                                                                                                                  ccAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGAcc                                    >  2:406242/1‑150 (MQ=255)
                                                                                                                   cAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATTGGGAGGTTAACGACGGTGAATATCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCt                                   >  1:2628890/1‑150 (MQ=255)
                                                                                                                   cAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCt                                   >  1:2409530/1‑150 (MQ=255)
                                                                                                                   cAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGGTTCCAGTGCCTGCGCAGGCGCCCGCGCCATGACCt                                   >  1:2071107/1‑150 (MQ=255)
                                                                                                                       tGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGt                               <  2:1713318/150‑1 (MQ=255)
                                                                                                                       tGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGGGACTGCGCAGGCGCCCGCGCCATGACCTGCGt                               <  2:2995032/150‑1 (MQ=255)
                                                                                                                         cAGGCGCTGGCGGTCGCCGACCACCGTCAGGGGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCCGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtg                             <  1:748857/150‑1 (MQ=255)
                                                                                                                          aGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCTGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgc                            <  1:1502532/150‑1 (MQ=255)
                                                                                                                          aGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgc                            >  2:2697590/1‑150 (MQ=255)
                                                                                                                           ggcgctggcgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgct                           >  2:4540970/1‑150 (MQ=255)
                                                                                                                                 ggcgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGt                     <  2:1374464/150‑1 (MQ=255)
                                                                                                                                 ggcgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGt                     <  1:4932394/150‑1 (MQ=255)
                                                                                                                                    gctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCa                  >  1:1441013/1‑150 (MQ=255)
                                                                                                                                    gatCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCGGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCa                  >  2:4763289/3‑150 (MQ=255)
                                                                                                                                      tCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCAtg                >  1:4021036/1‑150 (MQ=255)
                                                                                                                                           gACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGt           >  2:2032194/1‑150 (MQ=255)
                                                                                                                                           gACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGt           >  2:2032213/1‑150 (MQ=255)
                                                                                                                                            accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGt                     >  2:753557/1‑139 (MQ=255)
                                                                                                                                            accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTg          <  1:389434/150‑1 (MQ=255)
                                                                                                                                              caccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGc        >  1:1955662/1‑150 (MQ=255)
                                                                                                                                              caccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGc        >  1:736928/1‑150 (MQ=255)
                                                                                                                                                   tAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCt   >  1:3539564/1‑150 (MQ=255)
                                                                                                                                                    aaGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCtt  <  2:4264300/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCCACCTT  >  NC_002947/1148653‑1148950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑