BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	104,321	T→G	5.5%	I160M (ATT→ATG)	PP_0099 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	104,321	0	T	G	5.5%	606.7 / 18.5	184	I160M (ATT→ATG)	PP_0099	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (95/78); new base G (6/4); total (101/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 187 total aligned reads displayed.

CTGGCACAGCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGTGCGA  >  NC_002947/104172‑104469

     acaGCAATTGAGCACACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCAc                                                                                                                                                 <  2:2633697/150‑1 (MQ=255)

     acaGCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCCTTGTCAc                                                                                                                                                 >  1:2561292/1‑150 (MQ=255)

     acaGCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCAc                                                                                                                                                 >  2:3942507/1‑150 (MQ=255)

       aGCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCa                                                                                                                                               >  2:4273471/1‑150 (MQ=255)

        gCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACAATGGTCAccgg                                                                                                                                              <  1:1530729/150‑3 (MQ=255)

         cAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAg                                                                                                                                             >  2:3432625/1‑150 (MQ=255)

          aaTTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGGGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAgg                                                                                                                                            >  1:4143174/1‑150 (MQ=255)

          aaTTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGACCAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTGTTCGTCACCCGGTGACCATTGTCACCAAgg                                                                                                                                            >  2:3521701/1‑150 (MQ=255)

          aaTTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAgg                                                                                                                                            <  2:2204519/150‑1 (MQ=255)

          aaTTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAgg                                                                                                                                            <  2:2797813/150‑1 (MQ=255)

          aaTTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAgg                                                                                                                                            >  1:124650/1‑150 (MQ=255)

             tGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGtt                                                                                                                                         <  1:4716282/150‑1 (MQ=255)

               aGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCa                                                                                                                                       >  2:8104/1‑150 (MQ=255)

                gCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCAc                                                                                                                                      <  1:3574920/150‑1 (MQ=255)

                  aaaCCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTg                                                                                                                                    <  2:2948777/150‑1 (MQ=255)

                      cGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGtgc                                                                                                                                >  1:4351504/1‑150 (MQ=255)

                          cTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTgcg                                                                                                                            >  2:116669/1‑150 (MQ=255)

                          cTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTgcg                                                                                                                            >  2:4421823/1‑150 (MQ=255)

                           tACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTgcgc                                                                                                                           <  1:2726242/150‑1 (MQ=255)

                           tACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTgcgc                                                                                                                           <  2:4331200/150‑1 (MQ=255)

                                    gcgcCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGcgacctcgac                                                                                                                  >  1:4426585/1‑150 (MQ=255)

                                    gcgcCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGcgacctcgac                                                                                                                  >  1:1045579/1‑150 (MQ=255)

                                         gATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCGTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACctgct                                                                                                             <  2:1181426/150‑1 (MQ=255)

                                          aTCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACctgctg                                                                                                            >  1:4736243/1‑150 (MQ=255)

                                          aTCAATCTGGGCTCCAACACAGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCAACAAGGGTTCACTGGTGCTGCGCGACCTCGACctgctg                                                                                                            >  2:55401/1‑150 (MQ=255)

                                            cAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGCCCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCGGGc                                                                                                          <  2:3056078/150‑1 (MQ=255)

                                              aTCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCg                                                                                                        >  1:2201412/1‑150 (MQ=255)

                                               tCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCga                                                                                                       <  1:1190506/150‑1 (MQ=255)

                                                 tGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCgaga                                                                                                     >  1:4881339/1‑150 (MQ=255)

                                                 tGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCgaga                                                                                                     >  1:4881151/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  1:4133248/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  1:4823736/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  2:3135060/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  2:18617/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  1:1983856/1‑150 (MQ=255)

                                                        cAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCg                                                                                              >  1:954645/1‑150 (MQ=255)

                                                         aaCACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGc                                                                                             >  2:4036843/1‑150 (MQ=255)

                                                         aaCACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGc                                                                                             >  2:2716272/1‑150 (MQ=255)

                                                         aaCACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCAGTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGc                                                                                             >  2:1259725/1‑150 (MQ=255)

                                                            acCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAg                                                                                          >  1:4089156/1‑150 (MQ=255)

                                                                   cGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTgg                                                                                   >  1:240735/1‑150 (MQ=255)

                                                                      aCCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCAc                                                                                >  2:3634530/1‑150 (MQ=255)

                                                                       ccAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACg                                                                               <  1:3644204/150‑1 (MQ=255)

                                                                        cAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACgg                                                                              <  1:3381450/150‑1 (MQ=255)

                                                                        cAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTCGCACgg                                                                              >  2:1901004/1‑150 (MQ=255)

                                                                        aagacgatcgatCGCGAACAGTTACTGACCCTGCGCTTGCACGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACgg                                                                              >  2:4613408/5‑150 (MQ=255)

                                                                         aGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACggg                                                                             <  1:2321083/150‑1 (MQ=255)

                                                                          gCCGATCGGGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGt                                                                            <  2:4143174/150‑1 (MQ=255)

                                                                           ccGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGtg                                                                           >  1:3837166/1‑150 (MQ=255)

                                                                             gATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGtgat                                                                         <  2:51103/150‑1 (MQ=255)

                                                                                 gAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATc                                                                     <  1:2947734/150‑1 (MQ=255)

                                                                                  aGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCa                                                                    <  1:569337/150‑1 (MQ=255)

                                                                                  aGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCa                                                                    >  1:1240638/1‑150 (MQ=255)

                                                                                   gcgcGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAg                                                                   >  2:2502355/1‑150 (MQ=255)

                                                                                      cgAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCt                                                                <  1:944309/150‑1 (MQ=255)

                                                                                      cgAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCt                                                                <  2:8540/150‑1 (MQ=255)

                                                                                       gAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTg                                                               >  2:1260485/1‑150 (MQ=255)

                                                                                         aCAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGAc                                                             <  1:655977/150‑1 (MQ=255)

                                                                                           aGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCa                                                           <  1:3208694/150‑1 (MQ=255)

                                                                                               aCTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACg                                                                               <  2:3270241/126‑1 (MQ=255)

                                                                                               aCTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACg                                                                               >  1:704765/1‑126 (MQ=255)

                                                                                               aCTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCt                                                       >  1:2860504/1‑150 (MQ=255)

                                                                                                   aCCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGgac                                                   >  1:3790123/1‑150 (MQ=255)

                                                                                                      cGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGgacgac                                                <  1:2719145/150‑1 (MQ=255)

                                                                                                       ggCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGacgacg                                               <  2:259072/150‑1 (MQ=255)

                                                                                                              gCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCaa                                        >  2:3327122/1‑150 (MQ=255)

                                                                                                                tCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAAc                                      >  1:1954774/1‑150 (MQ=255)

                                                                                                                 cGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACg                                     >  1:5060591/1‑150 (MQ=255)

                                                                                                                  gAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACgg                                    <  2:4881339/150‑1 (MQ=255)

                                                                                                                  gAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACgg                                    <  2:1867522/150‑1 (MQ=255)

                                                                                                                   aaGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACggg                                   <  2:4409707/150‑1 (MQ=255)

                                                                                                                    aGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGt                                  <  2:1694370/150‑1 (MQ=255)

                                                                                                                      tgtTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGc                                >  1:1027509/1‑150 (MQ=255)

                                                                                                                        ttACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTc                              >  1:4409741/1‑150 (MQ=255)

                                                                                                                          aCTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGa                            >  1:4674265/1‑150 (MQ=255)

                                                                                                                           cTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGGCCAGCAGGTCgagg                                                                                                     >  1:1961336/1‑75 (MQ=255)

                                                                                                                           cTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGGCCAGCAGGTCgagg                                                                                                     <  2:1961336/76‑2 (MQ=255)

                                                                                                                           cTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGaa                           <  2:1515575/150‑1 (MQ=255)

                                                                                                                           cTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGaa                           >  2:22827/1‑150 (MQ=255)

                                                                                                                            tGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGc                                                                  <  2:930468/110‑1 (MQ=255)

                                                                                                                            tGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAAc                          >  2:626646/1‑150 (MQ=255)

                                                                                                                            tGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAAc                          >  1:2872007/1‑150 (MQ=255)

                                                                                                                              cGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAAccc                        >  1:4473790/1‑150 (MQ=255)

                                                                                                                               gTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAAcccc                       <  1:4260198/150‑1 (MQ=255)

                                                                                                                                 tttCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGa                     <  1:3942507/150‑1 (MQ=255)

                                                                                                                                      tCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGagcagc                >  2:4852902/1‑150 (MQ=255)

                                                                                                                                      tCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGagcagc                >  1:452809/1‑150 (MQ=255)

                                                                                                                                        aCCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCt              <  1:2875140/150‑1 (MQ=255)

                                                                                                                                        aCCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCt              >  1:4285885/1‑150 (MQ=255)

                                                                                                                                         cccGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCTCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTc             >  2:524690/1‑150 (MQ=255)

                                                                                                                                          ttggTGACAATGGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGtgat                                                                         >  2:691983/3‑89 (MQ=255)

                                                                                                                                          ttggTGACAATGGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCa            >  1:3277580/3‑150 (MQ=255)

                                                                                                                                          ttggTGACAATGGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCa            >  1:1753270/3‑150 (MQ=255)

                                                                                                                                             gTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCAccc         <  1:1848684/150‑1 (MQ=255)

                                                                                                                                              tGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCg        >  1:336172/1‑150 (MQ=255)

                                                                                                                                              tGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCg        >  1:336416/1‑150 (MQ=255)

                                                                                                                                                aCCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGt      <  2:264533/150‑1 (MQ=255)

                                                                                                                                                  cATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCCGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGTGc    >  2:479769/1‑150 (MQ=255)

                                                                                                                                                  cATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGTGc    >  1:4313991/1‑150 (MQ=255)

                                                                                                                                                   aTTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGTGCg   >  2:1374592/1‑150 (MQ=255)

CTGGCACAGCAATTGAGCAAACCGGGCTACGTGTGCGCGCCGATCAATCTGGGCTCCAACACCGACCCGTACCAGCCGATCGAGCGCGAACAGTTACTGACCCGGCGCTTGCTCGAAGTGTTACTGCGTTTTCGTCACCCGGTGACCATTGTCACCAAGGGTTCACTGGTGCTGCGCGACCTCGACCTGCTGGCCGAGATGGCCCGCCAGCGCCTGGCACGGGTGATGATCAGCCTGACCACGCTGGACGACGGCCTCAAACGGGTGCTCGAACCCCGAGCAGCCTCACCCGGTGCGA  >  NC_002947/104172‑104469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`