BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,380,640	T→C	5.4%	intergenic (‑22/+113)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,380,640	0	T	C	5.4%	406.1 / 7.1	132	intergenic (‑22/+113)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (36/85); new base C (4/3); total (44/88)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CAACAAACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGG  >  NC_002947/1380492‑1380789

caacaaACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAgtg                                                                                                                                                       >  1:4138858/1‑150 (MQ=255)

 aacaaACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTATAgtgt                                                                                                                                                      >  1:5018090/1‑150 (MQ=255)

 aacaaACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAgtgt                                                                                                                                                      >  2:2326623/1‑150 (MQ=255)

   caccccccGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGACTATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCTCCCGCGTCAGTGGGGCGGATTCTACAgtgtgt                                                                                                                                                    <  2:3150900/146‑1 (MQ=255)

   caaACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGACTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAgtgtgt                                                                                                                                                    >  2:2337834/1‑150 (MQ=255)

        cccGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGttt                                                                                                                                               <  1:2895119/150‑1 (MQ=255)

         ccGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTg                                                                                                                                              >  2:1883909/1‑150 (MQ=255)

          cgcgCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGt                                                                                                                                             <  2:4916991/150‑1 (MQ=255)

           gcgcTGCTTTTACCCCGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTa                                                                                                                                            <  1:2437676/150‑1 (MQ=255)

           gcgcTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTa                                                                                                                                            <  1:3597335/150‑1 (MQ=255)

             gctgctTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATg                                                                                                                                          >  1:3478652/1‑150 (MQ=255)

                  ttttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAg                                                                                                                                     <  2:3643939/150‑1 (MQ=255)

                  ttttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAg                                                                                                                                     >  1:1639803/1‑150 (MQ=255)

                  ttttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAg                                                                                                                                     >  2:2499807/1‑150 (MQ=255)

                  ttttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAg                                                                                                                                     <  2:1191228/150‑1 (MQ=255)

                  ttttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAg                                                                                                                                     <  2:1191469/150‑1 (MQ=255)

                   tttACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAgc                                                                                                                                    <  2:3914925/150‑1 (MQ=255)

                       cacaGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTa                                                                                                                                <  1:4387020/150‑1 (MQ=255)

                       cacaGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTa                                                                                                                                <  1:706610/150‑1 (MQ=255)

                        acaGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAgc                                                                                                                                    <  2:5038318/145‑1 (MQ=255)

                        acaGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAgc                                                                                                                                    >  1:5038318/1‑145 (MQ=255)

                             cGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCttt                                                                                                                          <  2:1368882/150‑1 (MQ=255)

                              ggggTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTATg                                                                                                                         >  2:2078771/1‑150 (MQ=255)

                                ggTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTATGAt                                                                                                                       <  1:2078771/150‑1 (MQ=255)

                                 gTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTTTGa                                                                                                                        <  1:2762019/148‑1 (MQ=255)

                                   ttGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGAtttt                                                                                                                    <  2:146970/150‑1 (MQ=255)

                                   ttGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGAtttt                                                                                                                    <  2:2080226/150‑1 (MQ=255)

                                    tGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGAttttt                                                                                                                   <  2:4033365/150‑1 (MQ=255)

                                    tGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGAttttt                                                                                                                   <  2:4033289/150‑1 (MQ=255)

                                      ttCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGttta                                                                                                                                              >  2:1657150/1‑120 (MQ=12)

                                      ttCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGAGTGTGGTTTGTATGTCAAGCGCTAGGCGTTGAGTTATCg                                                                                                                 >  1:497850/1‑150 (MQ=255)

                                          gACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGGTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑t                                                                                                            >  1:2518377/1‑150 (MQ=255)

                                               atatGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑tttttt                                                                                                       <  2:4185516/150‑1 (MQ=255)

                                                tatGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTc                                                                                                      <  2:545093/150‑1 (MQ=255)

                                                 atGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTcg                                                                                                     <  2:167634/150‑1 (MQ=255)

                                                  tGGTTGCGGGAGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGTCGAGCTACCAGGCTGCTCCATCCCTCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTcgc                                                                                                    <  1:1147967/150‑1 (MQ=255)

                                                  tGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTcgc                                                                                                    <  1:3305742/150‑1 (MQ=255)

                                                      ggCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAAt                                                                                                <  2:3962257/149‑1 (MQ=255)

                                                            aGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTAt                                                                                          <  1:4777414/150‑1 (MQ=255)

                                                              ccGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCGGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATca                                                                                        <  2:4305941/150‑1 (MQ=255)

                                                                ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATcagc                                                                                      >  2:3330718/1‑150 (MQ=255)

                                                                       aaCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCaaa                                                                               <  2:2440977/150‑1 (MQ=255)

                                                                          cgacgaCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGaa                                                                            >  1:144906/1‑150 (MQ=255)

                                                                            acgacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGaaaa                                                                          <  1:3216713/150‑1 (MQ=255)

                                                                                ccTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGc                                                                      <  1:1707919/150‑1 (MQ=255)

                                                                                ccTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGc                                                                      <  1:1630806/150‑1 (MQ=255)

                                                                                 cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGc                                                                      >  1:3624188/1‑150 (MQ=255)

                                                                                 cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGcc                                                                     >  1:751025/1‑150 (MQ=255)

                                                                                    cGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACt                                                                  <  2:1639803/150‑1 (MQ=255)

                                                                                    cGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACt                                                                  <  1:635158/150‑1 (MQ=255)

                                                                                    cGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACt                                                                  <  1:2206539/150‑1 (MQ=255)

                                                                                      ggTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGAGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACtgt                                                                >  1:4351723/1‑150 (MQ=255)

                                                                                           tGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTaa                                                           <  1:1686600/150‑1 (MQ=255)

                                                                                            gAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGt                                                                                                                                                 >  1:1055194/1‑64 (MQ=40)

                                                                                             aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                         <  1:2010667/150‑1 (MQ=255)

                                                                                               cccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGt                                                       <  1:3542456/150‑1 (MQ=255)

                                                                                               cccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGt                                                       <  1:1211528/150‑1 (MQ=255)

                                                                                               cccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTGGTTTGTATGTCAAGCGCt                                                                                                                                 >  1:2940295/1‑77 (MQ=21)

                                                                                                ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                      <  2:672681/150‑1 (MQ=255)

                                                                                                ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                      <  2:4245204/150‑1 (MQ=255)

                                                                                                 cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTgg                                                     <  1:1626738/150‑1 (MQ=255)

                                                                                                  gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                    <  1:4618367/150‑1 (MQ=255)

                                                                                                  gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                    <  1:959399/150‑1 (MQ=255)

                                                                                                  gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                    <  2:3060057/150‑1 (MQ=255)

                                                                                                  gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                    <  2:4940203/150‑1 (MQ=255)

                                                                                                  gacgaGCTACCAGACTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                    <  1:2337834/150‑1 (MQ=255)

                                                                                                      aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCttt                                                <  2:4822724/150‑1 (MQ=255)

                                                                                                          aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTCCAGTGTGTGGTTTGTATGTCAAGCGCTCGGCTTTGATTGTTCGACG‑TTCTTTCGCGAATGCTTATCAGCAGGCAACGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                            <  1:758675/150‑1 (MQ=18)

                                                                                                          aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCGAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                            <  2:2040933/150‑1 (MQ=255)

                                                                                                          aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                            <  1:2919081/150‑1 (MQ=255)

                                                                                                            cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTGGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTGTTTCGAGAAAGCTTATAATCAGGAAAATAAAAGCACCATTGTGTTAACAGTTGACTTTTATTGa                                          >  1:2374243/1‑150 (MQ=14)

                                                                                                             aGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGaa                                         >  1:3961722/1‑150 (MQ=255)

                                                                                                              ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAAt                                        >  1:3771661/1‑150 (MQ=255)

                                                                                                                ctgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCt                                      <  1:4505489/150‑1 (MQ=255)

                                                                                                                 tgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTg                                     <  1:3647421/150‑1 (MQ=255)

                                                                                                                  gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                    <  1:1883909/150‑1 (MQ=255)

                                                                                                                  gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                    <  1:837193/150‑1 (MQ=255)

                                                                                                                    tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCGTTTCTTGTATCTGGtt                                  >  1:4095433/1‑150 (MQ=255)

                                                                                                                     ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGG‑TTTTTTCTCGAATGATTATCAGCAGGCAAAGAAAAAGTCCACTGTGTAAAAAGTGGCATGTTCTTGAATCTGGTTg                                 >  1:1151517/1‑150 (MQ=18)

                                                                                                                        tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                              <  2:2983324/150‑1 (MQ=255)

                                                                                                                        tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                              <  1:3150850/150‑1 (MQ=255)

                                                                                                                        tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGAAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                              >  2:3527845/1‑150 (MQ=255)

                                                                                                                          ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGa                            <  2:4138858/150‑1 (MQ=255)

                                                                                                                                cAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGa                      <  1:3796290/150‑1 (MQ=255)

                                                                                                                                 aGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGACACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAt                     >  1:2852663/1‑150 (MQ=255)

                                                                                                                                  gTGGGGCGGATTCTACATTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                    <  1:2299102/150‑1 (MQ=255)

                                                                                                                                      ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                >  2:4768450/1‑150 (MQ=255)

                                                                                                                                       gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc               <  1:2499807/150‑1 (MQ=255)

                                                                                                                                          gATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACtgttaac                                                            <  2:717037/102‑5 (MQ=255)

                                                                                                                                          gATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcga            <  2:4066620/150‑1 (MQ=255)

                                                                                                                                           aTTCTACAGTTTGTGGTTTGTATGTCAAGCTCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACTGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgac           <  2:4850452/150‑1 (MQ=255)

                                                                                                                                            ttCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGTTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGACGGATTTGAACcgacg          >  2:2021041/1‑150 (MQ=255)

                                                                                                                                            ttCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTTGTTGCGGGAGCCGGATTTGAAacgacg          >  2:1768670/1‑150 (MQ=255)

                                                                                                                                            ttCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgacg          >  1:3963708/1‑150 (MQ=255)

                                                                                                                                               tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGGTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGTAAAAGGCCACTGTGTTAACAGTGGGCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAGCGACGAcc       >  2:1995529/1‑150 (MQ=255)

                                                                                                                                               tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc       >  2:74046/1‑150 (MQ=255)

                                                                                                                                               tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc       >  2:1320147/1‑150 (MQ=255)

                                                                                                                                                   gtgtgtGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg   <  1:404448/150‑1 (MQ=255)

                                                                                                                                                    tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAATAGGCCACTGTGTTAAGAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg  <  2:2644842/150‑1 (MQ=255)

                                                                                                                                                    tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg  <  1:74046/150‑1 (MQ=255)

CAACAAACCCCGCGCTGCTTTTACACAGGCGGGGTTTGTTCTGACGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACG‑TTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGG  >  NC_002947/1380492‑1380789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`