Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,549,807 C→G 8.8% S55S (TCC→TCG)  groL → chaperonin GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,549,8070CG 8.8% 546.5 / 33.7 170S55S (TCC→TCG) groLchaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base C (67/88);  new base G (5/10);  total (72/98)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 180 total aligned reads displayed.

GTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAA  >  NC_002947/1549658‑1549956
                                                                                                                                                     |                                                                                                                                                     
gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                       <  2:2910881/150‑1 (MQ=255)
gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                       >  2:4143932/1‑150 (MQ=255)
gTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTcc                                                                                                                                                       >  1:3587681/1‑150 (MQ=255)
   aaaTTTGGTTATTCCGCTCGTAAGAAATTGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTc                                                                                                                                                    <  1:4488917/150‑1 (MQ=255)
   aaaTTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTc                                                                                                                                                    <  2:3476753/150‑1 (MQ=255)
     aTTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGc                                                                                                                                                  <  1:402363/150‑1 (MQ=255)
         ggTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCaaa                                                                                                                                              <  1:689003/150‑1 (MQ=255)
             aTTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGaaa                                                                                                                                          >  1:164097/1‑150 (MQ=255)
                 cGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGa                                                                                                                                      <  2:2588093/150‑1 (MQ=255)
                  gCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAg                                                                                                                                     <  2:2724468/150‑1 (MQ=255)
                  gCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAg                                                                                                                                     >  2:3415498/1‑150 (MQ=255)
                    tCGTAAGAAATTGCTGGTTGGTGTCACCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCt                                                                                                                                   <  1:3531933/150‑1 (MQ=255)
                       tAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGaa                                                                                                                                >  1:3017406/1‑150 (MQ=255)
                       tAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGaa                                                                                                                                >  1:182665/1‑150 (MQ=255)
                          gAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGa                                                                                                                             >  1:4633368/1‑150 (MQ=255)
                                gCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCttt                                                                                                                       <  2:61415/150‑1 (MQ=255)
                                 cTGGTTGGTGTCCCCGTTCTGGCTGACGCGGGCAAAGCGACCCGGGGCCCGCAAGGCCGGAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCCTCACCAAGGACGGCGTTTCCGTCGCCACAGAAATCGCGCTGCAAGCCGcgggc                                                                                                                      >  2:876149/1‑146 (MQ=255)
                                  tggttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCg                                                                                                                     >  1:4059209/1‑150 (MQ=255)
                                  tggttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCg                                                                                                                     >  2:4822600/1‑150 (MQ=255)
                                  tggttggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTGTCg                                                                                                                     >  1:5040321/1‑150 (MQ=255)
                                       ggtGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAAc                                                                                                                >  2:1708743/1‑150 (MQ=255)
                                          gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                             <  2:4098076/150‑1 (MQ=255)
                                          gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                             <  2:4098107/150‑1 (MQ=255)
                                          gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                             >  1:2458108/1‑150 (MQ=255)
                                          gtCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATg                                                                                                             <  2:2481726/150‑1 (MQ=255)
                                           tCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATgg                                                                                                            >  1:977552/1‑150 (MQ=255)
                                           tCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATgg                                                                                                            <  1:4887129/150‑1 (MQ=255)
                                             aaCGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGgc                                                                                                          >  2:821297/1‑150 (MQ=255)
                                                      gCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTGTCGAAAACATGGGCGCCCAGcgg                                                                                                 >  2:3818540/1‑148 (MQ=255)
                                                        tGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGt                                                                                               >  1:478822/1‑150 (MQ=255)
                                                           cgcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCaa                                                                                            <  2:4540669/150‑1 (MQ=255)
                                                            gcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAg                                                                                           <  2:2611490/150‑1 (MQ=255)
                                                            gcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAg                                                                                           <  2:986892/150‑1 (MQ=255)
                                                            gcgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAg                                                                                           >  1:2265336/1‑150 (MQ=255)
                                                             cgGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAgg                                                                                          >  2:2084577/1‑150 (MQ=255)
                                                              ggTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGa                                                                                         <  2:151184/150‑1 (MQ=255)
                                                                tAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAg                                                                                       >  1:2486372/1‑150 (MQ=255)
                                                                tAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAg                                                                                       <  1:2676993/150‑1 (MQ=255)
                                                                tAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAg                                                                                       >  1:826195/1‑150 (MQ=255)
                                                                  aaaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGtt                                                                                     <  2:1128911/150‑1 (MQ=255)
                                                                   aaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCCAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                    >  2:2694434/1‑150 (MQ=255)
                                                                   aaGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTg                                                                                    >  1:4272344/1‑150 (MQ=255)
                                                                    aGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCCTCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGc                                                                                   >  1:3239919/1‑150 (MQ=255)
                                                                     gCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCt                                                                                  >  1:3780929/1‑150 (MQ=255)
                                                                          ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                             <  1:102829/150‑1 (MQ=255)
                                                                          ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                             >  1:1483374/1‑150 (MQ=255)
                                                                          ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                             <  1:5116843/150‑1 (MQ=255)
                                                                          ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                             >  2:4697917/1‑150 (MQ=255)
                                                                             gggCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGc                                                                          <  2:1666617/150‑1 (MQ=255)
                                                                                cccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCaa                                                                       >  1:4830216/1‑150 (MQ=255)
                                                                                 ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                      <  2:3131783/150‑1 (MQ=255)
                                                                                    aaaGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacgac                                                                   <  2:4265654/150‑1 (MQ=255)
                                                                                    aaaGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacgac                                                                   <  2:324538/150‑1 (MQ=255)
                                                                                           gTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                            >  2:1335860/1‑150 (MQ=255)
                                                                                           gTAACGTGGTACTGGACAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                            <  2:652320/150‑1 (MQ=255)
                                                                                                gTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGAc                                                       <  1:2373756/150‑1 (MQ=255)
                                                                                                  ggTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACgg                                                     <  1:4953044/150‑1 (MQ=255)
                                                                                                     aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                  <  1:2007647/150‑1 (MQ=255)
                                                                                                     aCTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcac                                                  <  1:2924720/150‑1 (MQ=255)
                                                                                                      cTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcacc                                                 <  2:2931576/150‑1 (MQ=255)
                                                                                                       tGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCAAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTACCAAGGCCAACGACGCTGCCGGTGACGGcacca                                                >  1:3354161/1‑150 (MQ=255)
                                                                                                          ccAAGAGCTTCGGCGCGCCGACCATCACCACGGACGGCGTTTCCGTCGCCAAAGAAAGCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacca                                             <  1:25522/150‑1 (MQ=255)
                                                                                                           cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGTCGCTGCCGGTGACGGcaccaccac                                            >  2:3169502/1‑150 (MQ=255)
                                                                                                           cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcacaaccac                                            >  1:4223407/1‑150 (MQ=255)
                                                                                                           cAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCAAAGGCCAACGACGCTGCCGGTGACGGcaccaccac                                            >  2:3301961/1‑150 (MQ=255)
                                                                                                            aagtccTTCGGCGCTCCACGCATCACCCAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACACGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGcaccaccact                                           <  2:2655268/145‑2 (MQ=255)
                                                                                                              gagCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGc                                         >  2:1798498/1‑150 (MQ=255)
                                                                                                              gagCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGc                                         <  2:2439735/150‑1 (MQ=255)
                                                                                                                gCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTa                                       <  1:73660/150‑1 (MQ=255)
                                                                                                                  ttCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAcc                                     >  1:4173756/1‑150 (MQ=255)
                                                                                                                       cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                <  2:5063796/150‑1 (MQ=255)
                                                                                                                        gcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAACTCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGACGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTg                               <  2:73198/150‑1 (MQ=255)
                                                                                                                         cgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                              <  1:3036130/150‑1 (MQ=255)
                                                                                                                            cGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTc                           >  1:1079984/1‑150 (MQ=255)
                                                                                                                             gACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCa                          <  2:181912/150‑1 (MQ=255)
                                                                                                                               gcATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAgg                        >  2:3579089/2‑150 (MQ=255)
                                                                                                                                cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                       >  2:514762/1‑150 (MQ=255)
                                                                                                                                cATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGc                       <  2:2293127/150‑1 (MQ=255)
                                                                                                                                  tCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGcta                     <  2:4427236/150‑3 (MQ=255)
                                                                                                                                   cACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCAt                    >  2:3666998/1‑150 (MQ=255)
                                                                                                                                     ccAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCg                  >  2:4501539/1‑150 (MQ=255)
                                                                                                                                      cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                 >  1:2187681/1‑150 (MQ=255)
                                                                                                                                      cAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                 <  1:444202/150‑1 (MQ=255)
                                                                                                                                       aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTc                <  1:4337256/150‑1 (MQ=255)
                                                                                                                                        aGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttc               <  2:906640/150‑3 (MQ=255)
                                                                                                                                         ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca              <  2:701142/150‑4 (MQ=255)
                                                                                                                                         ggACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCaa              <  2:4902760/150‑1 (MQ=255)
                                                                                                                                           aCGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttcagg            <  1:1412907/150‑6 (MQ=255)
                                                                                                                                             ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa          <  1:3036706/150‑1 (MQ=255)
                                                                                                                                             ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa          <  2:2430855/150‑1 (MQ=255)
                                                                                                                                              gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAg         <  2:2920658/150‑1 (MQ=255)
                                                                                                                                              gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAg         <  2:2636610/150‑1 (MQ=255)
                                                                                                                                                gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGc       <  1:4143932/150‑1 (MQ=255)
                                                                                                                                                 tttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGcc      <  2:4059209/150‑1 (MQ=255)
                                                                                                                                                  ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCt     <  2:3587681/150‑1 (MQ=255)
                                                                                                                                                  ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCt     <  2:1885434/150‑1 (MQ=255)
                                                                                                                                                    ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa   <  1:768683/150‑1 (MQ=255)
                                                                                                                                                    ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa   <  1:3415498/150‑1 (MQ=255)
                                                                                                                                                    ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa   <  2:3366777/150‑1 (MQ=255)
                                                                                                                                                     cGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaa  >  2:2353555/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAAAATTTGGTGATTCCGCTCGTAAGAAAATGCTGGTTGGTGTCAACGTTCTGGCTGACGCGGTAAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAA  >  NC_002947/1549658‑1549956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑