Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,5340GC40.5% 168.2 / 224.8 168intergenic (+51/+63)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base G (38/62);  new base C (32/36);  total (70/98)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

ATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGAT  >  NC_002947/1544386‑1544680
                                                                                                                                                     |                                                                                                                                                    
aTTTTGCCCTGGGGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGGGGTTTGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTCAACCCG‑CTCCTACACAggg                                                                                                                                                     <  2:3777774/150‑1 (MQ=255)
aTTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGGGGCCGCTGTGGTTTGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAggg                                                                                                                                                     <  2:3225147/150‑1 (MQ=255)
aTTGTGCCCGGGGGGGGGGGGGGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAggg                                                                                                                                                     <  1:1983825/150‑1 (MQ=39)
 ttttGCCGTGGCGGGGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGgc                                                                                                                                                    <  2:5407792/150‑1 (MQ=255)
 ttttGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGGGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGgc                                                                                                                                                    <  1:1286560/150‑1 (MQ=255)
       ccTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCGCGTCGCGGGGGAAACCC‑CGCCCACACAGGgccccgcc                                                                                                                                              >  2:2507512/1‑150 (MQ=255)
            ggggggggggggggggggCGTGGGCGCTGGGGCGGCGGGGGTTGGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATCATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                        <  1:162213/146‑1 (MQ=37)
             gggggggggggggggggCGTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTg                                                                                                                                       <  1:5182383/147‑1 (MQ=255)
              ggggggggggggggggggTGGGGGGGGGGGCCGCTGTGGTTTGTGGGGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                      <  1:3866167/148‑1 (MQ=38)
              ggggggggggggggggCGGTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                      <  2:3058130/148‑1 (MQ=255)
              gggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑Tgtgt                                                                                                                                      <  2:183510/148‑1 (MQ=37)
               gggggggggggggggggTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                     <  1:3344921/149‑1 (MQ=255)
               gggggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  1:1519618/149‑1 (MQ=37)
               gggggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  1:4929593/149‑1 (MQ=37)
               gggggggggggggggCGTGGGCGCGGGGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  1:1937536/149‑1 (MQ=25)
               gggggggggggggggCGTGGGCGCGGGGGCCGCGGTGGTTTGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                     <  1:3344600/149‑1 (MQ=38)
               ggggggTGGGGGGGGGGTTGGCGGTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                     <  1:301447/149‑1 (MQ=255)
               ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTATGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  2:9644/149‑1 (MQ=37)
               ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGTTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  1:2010477/149‑1 (MQ=37)
               ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                     <  1:1328128/149‑1 (MQ=255)
               ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  2:1710200/149‑1 (MQ=37)
               cggGGGGGGCGGGGGCGTGGGCGCTGTGGCCGCTATGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                     <  2:2722914/150‑1 (MQ=25)
                ggggggggggggggggTGGGCGCTGGGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                    <  1:2267948/150‑1 (MQ=25)
                ggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAg                                                                                                                                    <  2:1391149/150‑1 (MQ=255)
                gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                    <  1:57303/150‑1 (MQ=25)
                gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACGTCGCGGGTAAACCCC‑CCCCTACACAGGGCCGCGCCGCGTTTGtg                                                                                                                                     >  2:2528102/1‑149 (MQ=255)
                  ggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                  <  1:2690189/150‑1 (MQ=25)
                  ggggggggggggCGTTGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                  <  2:2394028/150‑1 (MQ=25)
                  ggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                  <  2:3472319/150‑1 (MQ=37)
                  gggTGGCGGGGGCGTTGGCGCTTTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                  <  2:2380135/150‑1 (MQ=255)
                  gggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑tt                                                                                                                                         <  2:1821442/143‑1 (MQ=255)
                  gggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                  <  2:3798017/150‑1 (MQ=255)
                   ggTGGCGGGGGTGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAg                                                                                                                                 <  1:3519659/150‑1 (MQ=255)
                    gTGGGGGGGGGGTTGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGc                                                                                                                                <  1:2078760/150‑1 (MQ=255)
                    gTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGc                                                                                                                                <  1:4360174/150‑1 (MQ=255)
                      gggggggggGTTGGCGCTGGGGCCGCTGGGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCgg                                                                                                                              <  1:4303073/147‑1 (MQ=38)
                      ggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCgg                                                                                                                              <  1:1910792/147‑1 (MQ=25)
                      ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCgg                                                                                                                              <  1:5326912/150‑1 (MQ=37)
                      ggCGGGGGCGTGGGCGCTGTGGCCGCTGGGGGTGGTGGTGGGGGGGCGGCCGGGGGACGGGGCTGGAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCgg                                                                                                                              <  2:3681045/150‑1 (MQ=21)
                       ggggggggGTTGGCGCTGTGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                             <  1:2785374/148‑1 (MQ=255)
                       gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                             <  1:607835/148‑1 (MQ=21)
                       gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                             <  1:997204/148‑1 (MQ=255)
                       gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                             <  1:1994157/148‑1 (MQ=25)
                       gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                             <  2:4119282/150‑1 (MQ=21)
                            ggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑c                                                                                                                       <  2:3427512/150‑1 (MQ=255)
                               gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCcg                                                                                                                    <  2:705396/150‑1 (MQ=255)
                                   gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                    <  1:3227686/131‑1 (MQ=18)
                                   gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                    >  2:3227686/1‑131 (MQ=18)
                                     gcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                             >  1:2136720/1‑136 (MQ=255)
                                     gcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                             <  2:2136720/136‑1 (MQ=255)
                                     gcTGTGGCCGCTGTGGTTGGTGGGGGGTGGGCGGCCTGGGGAAGGGGCGGTAGCTCGATAATCGGTATGGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACCGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAaga                                                                                                              <  2:4712548/150‑1 (MQ=38)
                                         gggcggcTGGGGTTGGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATGGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGcc                                                                                                          <  2:3082817/149‑1 (MQ=255)
                                          ggcgggtgtgGTTGGTGGGGGGGGGGGGGCGTGGGGTGGGGGCTGTAGCTCTATATGCGGTATTGCGGGGGCCGGCCTCTTCGGGGGTAACCCCG‑CTCCTACACTGGGCGGGGCCGT‑TTTGTAGGAGGGGTTTTA‑CCCGCGAAGAGGCCa                                                                                                         <  2:243508/144‑1 (MQ=19)
                                                 gtgGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                      <  1:5060834/115‑1 (MQ=255)
                                                      ttGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCggcgcggccct                                                                                                                                           <  1:3423648/106‑6 (MQ=25)
                                                                 gggCGGCCTGGGGACGGGGCTGTAGCTCGATAATCGGTCTGGCGGGGGCCGGCCTCTTCGGGGTTAAACCCG‑CCCCTACACCGGGCCGCGCCGC‑TTTGTCGGAGGGGTTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAg                                                                                  <  2:1452865/150‑1 (MQ=38)
                                                                            ggACGGGGCTGTAGCTCGATAATCGGTTTTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGc                                                                       <  2:5274742/150‑1 (MQ=255)
                                                                                         gCTCGATAATCGGTATTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                  >  1:1935394/1‑79 (MQ=255)
                                                                                         gCTCGATAATCGGTATTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                  <  2:1935394/79‑1 (MQ=255)
                                                                                                aaacGGTACGGCCGGGGCCGGCCCCTCCGCGGCACACCCCG‑CTCCTACACAGGGCCGCGCCGA‑TTTGTAGGAGGGGTTTGA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCa                                                   <  1:865713/147‑1 (MQ=255)
                                                                                                             ggggCGGGCCTTTTGGCGTTTAAACCGG‑CTCCTACACTGGGCGGGGCGTT‑TTTGTTGTTGGGGTTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccg                                      <  1:1876996/150‑1 (MQ=255)
                                                                                                                   ggccTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGCAGAGGCCAGCACAGGCAATACAAAGCGTCAGGGCCGCTCAGCCCCCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGc                                >  2:313791/1‑150 (MQ=18)
                                                                                                                     ccTCTTCGCGGGTAACCCCG‑CTCCTACACTGGGCGGCGCCGC‑TTGGTAGGAGCGGTTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCtt                              <  2:2770020/150‑1 (MQ=39)
                                                                                                                      ctctTCGCGGGTAAACCCC‑CGCCTACAAAGCGGCGCGGCCC‑GGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAAAACAAAGCTTCAGGGCCGCTCCGCCACCCAGTGGTCGTCCCCCACCGGCGTCCCCCCCCCGGCGTc                             >  2:1887021/1‑150 (MQ=18)
                                                                                                                            gcgGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCTGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                       >  1:4765205/1‑150 (MQ=25)
                                                                                                                            gcgGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                       >  1:5236821/1‑150 (MQ=25)
                                                                                                                             ggggTAAACCCGCCTCCCACCCGGGGCCGCGCCGT‑TTTGTCGG‑GGGGGTTTACCCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                       <  1:230276/149‑1 (MQ=255)
                                                                                                                                  aaaCCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg                 >  2:4949382/1‑150 (MQ=255)
                                                                                                                                    ccccG‑CCCCTCCCCGGGGCCGCGCGGC‑TTTTCTGGCG‑GGGTTTACCCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa               <  1:2507512/149‑1 (MQ=255)
                                                                                                                                     cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg              >  1:3493865/1‑150 (MQ=255)
                                                                                                                                     cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg              >  2:5359398/1‑150 (MQ=255)
                                                                                                                                     cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg              >  1:391117/1‑150 (MQ=255)
                                                                                                                                     cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                      >  2:399116/1‑142 (MQ=25)
                                                                                                                                     cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                      <  1:399116/142‑1 (MQ=25)
                                                                                                                                     cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg              >  1:698522/1‑150 (MQ=37)
                                                                                                                                      ccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg             >  2:4268846/1‑150 (MQ=255)
                                                                                                                                      ccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg             >  2:4258227/1‑150 (MQ=255)
                                                                                                                                      ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg             >  2:1461867/1‑150 (MQ=37)
                                                                                                                                      ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg             >  1:475474/1‑150 (MQ=37)
                                                                                                                                       cG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg            >  1:1751592/1‑150 (MQ=255)
                                                                                                                                        g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                              <  1:2844587/99‑1 (MQ=17)
                                                                                                                                        g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                              >  2:2844587/1‑99 (MQ=17)
                                                                                                                                        g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc           >  1:64601/1‑150 (MQ=37)
                                                                                                                                           tCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga         >  2:2230183/1‑150 (MQ=255)
                                                                                                                                           tCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga         >  1:3670204/1‑150 (MQ=255)
                                                                                                                                           tCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga         >  1:4483501/1‑150 (MQ=255)
                                                                                                                                           tCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga         >  1:4171452/1‑150 (MQ=255)
                                                                                                                                           tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                              <  2:5123815/97‑1 (MQ=17)
                                                                                                                                           tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga         >  1:29680/1‑150 (MQ=37)
                                                                                                                                             cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc       >  2:4882623/1‑150 (MQ=37)
                                                                                                                                             cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc       >  1:3889137/1‑150 (MQ=37)
                                                                                                                                              tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  1:4079352/1‑150 (MQ=255)
                                                                                                                                              tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  1:1640955/1‑150 (MQ=255)
                                                                                                                                              tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  1:249998/1‑150 (MQ=255)
                                                                                                                                              tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  1:3885798/1‑150 (MQ=255)
                                                                                                                                              tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  2:1943924/1‑150 (MQ=255)
                                                                                                                                              tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  2:1319779/1‑150 (MQ=37)
                                                                                                                                              tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca      >  2:3678284/1‑150 (MQ=37)
                                                                                                                                                cacaGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcagg    >  1:3969031/1‑150 (MQ=255)
                                                                                                                                                  ccgggCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggat  <  2:3706796/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGAT  >  NC_002947/1544386‑1544680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑