Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,5430GC41.9% 128.4 / 221.0 162intergenic (+60/+54)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base G (36/57);  new base C (32/35);  total (68/94)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

CGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCA  >  NC_002947/1544398‑1544691
                                                                                                                                                  |                                                                                                                                                      
ggggggggggggggggggCGTGGGCGCTGGGGCGGCGGGGGTTGGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATCATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                                   <  1:162213/146‑1 (MQ=37)
 gggggggggggggggggCGTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTg                                                                                                                                                  <  1:5182383/147‑1 (MQ=255)
 ggggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTg                                                                                                                                                  <  1:3873224/147‑1 (MQ=255)
  ggggggggggggggggCGGTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                                 <  2:3058130/148‑1 (MQ=255)
  gggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑Tgtgt                                                                                                                                                 <  2:183510/148‑1 (MQ=37)
  gcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑Tgtgt                                                                                                                                                 <  2:2194468/150‑1 (MQ=37)
   gggggggggggggggggTGGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                                <  1:3344921/149‑1 (MQ=255)
   gggggggggggggggCGTTGGCGCTGTGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  1:4252042/149‑1 (MQ=25)
   gggggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  1:1519618/149‑1 (MQ=37)
   gggggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  1:4929593/149‑1 (MQ=37)
   gggggggggggggggCGTGGGCGCGGGGGCCGCGGTGGTTTGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                                <  1:3344600/149‑1 (MQ=38)
   gggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATACTCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                                <  2:5335630/149‑1 (MQ=255)
   ggggggTGGGGGGGGGGTTGGCGGTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                                <  1:301447/149‑1 (MQ=255)
   ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTATGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  2:9644/149‑1 (MQ=37)
   ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGTTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  1:2010477/149‑1 (MQ=37)
   ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  2:1710200/149‑1 (MQ=37)
   cggGGGGGGCGGGGGCGTGGGCGCTGTGGCCGCTATGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                                <  2:2722914/150‑1 (MQ=25)
    ggggggggggggggggTGGGCGCTGGGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                               <  1:2267948/150‑1 (MQ=25)
    ggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAg                                                                                                                                               <  2:1391149/150‑1 (MQ=255)
    gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCTGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                               <  1:1721555/150‑1 (MQ=21)
    gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                               <  1:57303/150‑1 (MQ=25)
    gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAg                                                                                                                                               <  1:3564287/150‑1 (MQ=255)
    gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACGTCGCGGGTAAACCCC‑CCCCTACACAGGGCCGCGCCGCGTTTGtg                                                                                                                                                >  2:2528102/1‑149 (MQ=255)
      ggggggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                             <  1:2690189/150‑1 (MQ=25)
      ggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                             <  2:3472319/150‑1 (MQ=37)
      gggTGGGGGGGGCGTTGGCGCTGGGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGa                                                                                                                                             <  2:2394096/150‑1 (MQ=25)
      gggTGGCGGGGGCGTTGGCGCTTTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                             <  2:2380135/150‑1 (MQ=255)
      gggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑tt                                                                                                                                                    >  1:1821442/1‑143 (MQ=255)
      gggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑tt                                                                                                                                                    <  2:1821442/143‑1 (MQ=255)
      gggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                             <  2:3798017/150‑1 (MQ=255)
       ggTGGCGGGGGTGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAg                                                                                                                                            <  1:3519659/150‑1 (MQ=255)
        gTGGGGGGGGGGTTGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGc                                                                                                                                           <  1:2078760/150‑1 (MQ=255)
        gTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGc                                                                                                                                           <  1:3445759/150‑1 (MQ=37)
        gTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGc                                                                                                                                           <  1:4360174/150‑1 (MQ=255)
         gggggggggCGTGGGCGCGGGGGCCGCGGGGGTTGGGGGGGGGGGGGCGGCCGGGGGACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCg                                                                                                                                          <  1:1907133/146‑1 (MQ=37)
          ggggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCgg                                                                                                                                         <  1:1910792/147‑1 (MQ=25)
          ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCgg                                                                                                                                         <  1:5326912/150‑1 (MQ=37)
           ggggggggGTTGGCGCTGTGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                                        <  1:2785374/148‑1 (MQ=255)
           gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                                        <  1:607835/148‑1 (MQ=21)
           gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                                        <  1:997204/148‑1 (MQ=255)
           gggggggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                                        <  1:1994157/148‑1 (MQ=25)
           gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCggg                                                                                                                                        <  2:4119282/150‑1 (MQ=21)
                ggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑c                                                                                                                                  <  2:3427512/150‑1 (MQ=255)
                   gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCcg                                                                                                                               <  2:705396/150‑1 (MQ=255)
                       gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                               <  1:3227686/131‑1 (MQ=18)
                       gcgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                               >  2:3227686/1‑131 (MQ=18)
                         gcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCggg                                                                                                                                        >  1:2136720/1‑136 (MQ=255)
                             gggcggcTGGGGTTGGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATGGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGcc                                                                                                                     <  2:3082817/149‑1 (MQ=255)
                                     gtgGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                                 <  1:5060834/115‑1 (MQ=255)
                                     gtgGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                                 >  2:2341068/1‑115 (MQ=255)
                                                     gggCGGCCTGGGGACGGGGCTGTAGCTCGATAATCGGTCTGGCGGGGGCCGGCCTCTTCGGGGTTAAACCCG‑CCCCTACACCGGGCCGCGCCGC‑TTTGTCGGAGGGGTTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAg                                                                                             <  2:1452865/150‑1 (MQ=38)
                                                                             gCTCGATAATCGGTATTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                             >  1:1935615/1‑79 (MQ=255)
                                                                             gCTCGATAATCGGTATTGCCTGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                             <  2:1935394/79‑1 (MQ=255)
                                                                                    aaacGGTACGGCCGGGGCCGGCCCCTCCGCGGCACACCCCG‑CTCCTACACAGGGCCGCGCCGA‑TTTGTAGGAGGGGTTTGA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCa                                                              <  1:865713/147‑1 (MQ=255)
                                                                                              gCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTccc                                                    <  2:3979516/150‑1 (MQ=255)
                                                                                                       ggccTCTTCGCGGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGCAGAGGCCAGCACAGGCAATACAAAGCGTCAGGGCCGCTCAGCCCCCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGc                                           >  2:313791/1‑150 (MQ=18)
                                                                                                         ccTCTTCGCGGGTAACCCCG‑CTCCTACACTGGGCGGCGCCGC‑TTGGTAGGAGCGGTTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCtt                                         <  2:2770020/150‑1 (MQ=39)
                                                                                                          ctctTCGCGGGTAAACCCC‑CGCCTACAAAGCGGCGCGGCCC‑GGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAAAACAAAGCTTCAGGGCCGCTCCGCCACCCAGTGGTCGTCCCCCACCGGCGTCCCCCCCCCGGCGTc                                        >  2:1887021/1‑150 (MQ=18)
                                                                                                                gcgGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCTGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                                  >  1:4765205/1‑150 (MQ=25)
                                                                                                                gcgGGTAAACCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                                  >  1:5236821/1‑150 (MQ=25)
                                                                                                                 ggggTAAACCCGCCTCCCACCCGGGGCCGCGCCGT‑TTTGTCGG‑GGGGGTTTACCCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATg                                  <  1:230276/149‑1 (MQ=255)
                                                                                                                      aaaCCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg                            >  2:4949382/1‑150 (MQ=255)
                                                                                                                        ccccG‑CCCCTCCCCGGGGCCGCGCGGC‑TTTTCTGGCG‑GGGTTTACCCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa                          <  1:2507512/149‑1 (MQ=255)
                                                                                                                        aCCCG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa                          >  1:388927/1‑150 (MQ=25)
                                                                                                                         cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  1:3698122/1‑150 (MQ=255)
                                                                                                                         cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  2:5359398/1‑150 (MQ=255)
                                                                                                                         cccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  1:391290/1‑150 (MQ=255)
                                                                                                                         cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                                 >  2:399116/1‑142 (MQ=25)
                                                                                                                         cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                                 <  1:399116/142‑1 (MQ=25)
                                                                                                                         cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  2:2439377/1‑150 (MQ=37)
                                                                                                                         cccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                         >  1:698522/1‑150 (MQ=37)
                                                                                                                          ccG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  2:4258227/1‑150 (MQ=255)
                                                                                                                          ccG‑CTCCTACACAGGGCCGCGCCGC‑GTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCATCAGGGCCGCTCCGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTACCTGATGCCACTGCAgg                        >  2:4427416/1‑150 (MQ=255)
                                                                                                                          ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  2:1461867/1‑150 (MQ=37)
                                                                                                                          ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  2:3705656/1‑150 (MQ=37)
                                                                                                                          ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  1:475474/1‑150 (MQ=37)
                                                                                                                          ccG‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                        >  2:4718780/1‑150 (MQ=37)
                                                                                                                            g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                                         >  2:2844587/1‑99 (MQ=17)
                                                                                                                            g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                                         <  1:2844587/99‑1 (MQ=17)
                                                                                                                            g‑CTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc                      >  1:64601/1‑150 (MQ=37)
                                                                                                                               tCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga                    >  1:4483501/1‑150 (MQ=255)
                                                                                                                               tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg                                                                         <  2:5123815/97‑1 (MQ=17)
                                                                                                                               tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga                    >  1:29680/1‑150 (MQ=37)
                                                                                                                                 cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc                  >  1:3889137/1‑150 (MQ=37)
                                                                                                                                  tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:249998/1‑150 (MQ=255)
                                                                                                                                  tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  2:1943924/1‑150 (MQ=255)
                                                                                                                                  tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:1640955/1‑150 (MQ=255)
                                                                                                                                  tACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:4079363/1‑150 (MQ=255)
                                                                                                                                  tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  2:3678284/1‑150 (MQ=37)
                                                                                                                                  tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:1980760/1‑150 (MQ=37)
                                                                                                                                  tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  2:1319779/1‑150 (MQ=37)
                                                                                                                                  tACAAAGCGGCGCGGCCC‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca                 >  1:3993418/1‑150 (MQ=37)
                                                                                                                                      ccgggCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggat             <  2:3706796/148‑1 (MQ=255)
                                                                                                                                       agcggcgcggccc‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTcccc                                                   <  1:4377138/106‑1 (MQ=25)
                                                                                                                                       agcggcgcggccc‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTcccc                                                   >  2:4377138/6‑111 (MQ=25)
                                                                                                                                       agcggcgcggccc‑TGTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatc            >  2:5208309/6‑150 (MQ=255)
                                                                                                                                             gcgccgc‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTa      >  2:4968696/1‑150 (MQ=255)
                                                                                                                                               gccgc‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAgg    >  1:119488/1‑150 (MQ=255)
                                                                                                                                               gccgc‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAgg    >  1:119661/1‑150 (MQ=255)
                                                                                                                                                 cgc‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCGGCGTAGGCa  >  2:1935824/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTAGGCA  >  NC_002947/1544398‑1544691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑