Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,484,303 T→G V125G (GTC→GGC)  yhdX → amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,484,3030TG98.2% 776.5 / 1.9 220V125G (GTC→GGC) yhdXamino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base G (112/104);  total (116/104)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 220 total aligned reads displayed.

CCGACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATGCCTG  >  NC_002947/1484156‑1484449
                                                                                                                                                   |                                                                                                                                                  
ccGACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCt                                                                                                                                                  <  2:447935/150‑1 (MQ=255)
ccGACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCt                                                                                                                                                  >  2:1003750/1‑150 (MQ=255)
  gACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTAt                                                                                                                                                >  1:3111364/1‑150 (MQ=255)
  gACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTAt                                                                                                                                                >  2:2144246/1‑150 (MQ=255)
       ttATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGa                                                                                                                                           >  2:3405233/1‑150 (MQ=255)
             gcgGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCtt                                                                                                                                     >  1:352976/1‑150 (MQ=255)
                   gtTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCaa                                                                                                                               <  1:4310689/150‑1 (MQ=255)
                         cATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTcc                                                                                                                         <  2:2408091/150‑1 (MQ=255)
                          aTCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccg                                                                                                                        >  1:1632473/1‑150 (MQ=255)
                           tCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccgc                                                                                                                       <  2:3460576/150‑1 (MQ=255)
                           tCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTccgc                                                                                                                       >  2:2281959/1‑150 (MQ=255)
                                cTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCgttg                                                                                                                  >  2:3782444/1‑150 (MQ=255)
                                 tGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCgttgt                                                                                                                 >  1:3909540/1‑150 (MQ=255)
                                  gCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCgttgtt                                                                                                                <  1:1263362/150‑1 (MQ=255)
                                    tCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGc                                                                                                              >  2:2034475/1‑150 (MQ=255)
                                    tCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGc                                                                                                              <  1:2456994/150‑1 (MQ=255)
                                      aaTACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAg                                                                                                            >  2:4077445/1‑150 (MQ=255)
                                         aCGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATc                                                                                                         <  2:5060974/150‑1 (MQ=255)
                                          cGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATcc                                                                                                        <  2:1041902/150‑1 (MQ=255)
                                           gCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCt                                                                                                       <  2:1109360/150‑1 (MQ=255)
                                            cTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTg                                                                                                      <  1:3305218/150‑1 (MQ=255)
                                             tGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGt                                                                                                     <  2:23848/150‑1 (MQ=255)
                                                 ggTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTg                                                                                                 <  1:3200468/150‑1 (MQ=255)
                                                 ggTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTg                                                                                                 >  1:704906/1‑150 (MQ=255)
                                                 ggTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTg                                                                                                 <  2:5269706/150‑1 (MQ=255)
                                                  gTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTgg                                                                                                >  1:2093965/1‑150 (MQ=255)
                                                    gACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTa                                                                                              <  1:2014511/150‑1 (MQ=255)
                                                       cTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTAGTTGCAGATACTGTACTGGTAttt                                                                                           >  2:1042532/1‑150 (MQ=255)
                                                       cTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTAttt                                                                                           >  1:170425/1‑150 (MQ=255)
                                                       cTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTAttt                                                                                           >  1:1077589/1‑150 (MQ=255)
                                                            tCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCg                                                                                      >  2:1027365/1‑150 (MQ=255)
                                                            tCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTACGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCg                                                                                      >  1:4642451/1‑150 (MQ=255)
                                                            tCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATATGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCg                                                                                      >  2:361049/1‑150 (MQ=255)
                                                             cGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgt                                                                                     >  1:4553524/1‑150 (MQ=255)
                                                             cGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgt                                                                                     <  1:302625/150‑1 (MQ=255)
                                                             cGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgt                                                                                     <  1:4772852/150‑1 (MQ=255)
                                                             cGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgt                                                                                     <  1:5293537/150‑1 (MQ=255)
                                                               gCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgtgt                                                                                   <  2:4737006/150‑1 (MQ=255)
                                                               gCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCgtgt                                                                                   <  1:5244661/150‑1 (MQ=255)
                                                                 gTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTc                                                                                 <  2:2066650/150‑1 (MQ=255)
                                                                  tCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTcc                                                                                >  2:5172904/1‑150 (MQ=255)
                                                                     ttCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGa                                                                             <  2:170425/150‑1 (MQ=255)
                                                                     ttCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGa                                                                             <  2:2669093/150‑1 (MQ=255)
                                                                     ttCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGa                                                                             <  1:622413/150‑1 (MQ=255)
                                                                     ttCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGa                                                                             >  1:5397380/1‑150 (MQ=255)
                                                                      tCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGAc                                                                            <  1:4553168/150‑1 (MQ=255)
                                                                            gacgaTACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGcc                                                                      <  1:1633078/150‑1 (MQ=255)
                                                                                aTACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGa                                                                  <  1:4352489/150‑1 (MQ=255)
                                                                                    tGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACcgc                                                              >  2:1898118/1‑150 (MQ=255)
                                                                                      ggCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGAGCCTGCCGGGACCGCgg                                                            >  2:159818/1‑150 (MQ=255)
                                                                                         ttCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgc                                                         <  2:4923383/150‑1 (MQ=255)
                                                                                         ttCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgc                                                         <  2:64153/150‑1 (MQ=255)
                                                                                            atcatcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgcagc                                                      <  2:3096954/150‑1 (MQ=255)
                                                                                            atcatcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgcagc                                                      <  2:381216/150‑1 (MQ=255)
                                                                                             tcatcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGgcagca                                                     >  1:953981/1‑150 (MQ=255)
                                                                                               atcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATc                                                   >  2:836209/1‑150 (MQ=255)
                                                                                               atcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATc                                                   >  1:3239796/1‑150 (MQ=255)
                                                                                               atcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATc                                                   <  1:4902494/150‑1 (MQ=255)
                                                                                                tcGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGGTGTTGCAGATCCTGGTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCa                                                  >  2:4469186/1‑150 (MQ=255)
                                                                                                  ggTGTGGCTCGCCTGTGACCGAACTGGATGATCAACAAGCTGGCGAGCGGGTATGTGGAATCCTTCCGCAACATTCCGCGGTTGTTGGTGTTCGTGTTGTGGTATTTCGCGGTGTTCGTGTCGCTGCCGGGAGCGCGGGGCAGCATCaag                                                >  2:5420329/1‑149 (MQ=255)
                                                                                                    tgtgGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACAt                                              >  2:1443952/1‑150 (MQ=255)
                                                                                                       ggCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGa                                           <  1:4458917/150‑1 (MQ=255)
                                                                                                          tCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGGCCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGa                                        <  1:1461811/150‑1 (MQ=255)
                                                                                                          tCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGa                                        >  2:2829480/1‑150 (MQ=255)
                                                                                                          tCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGa                                        <  1:2358174/150‑1 (MQ=255)
                                                                                                             ccTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACAt                                     >  2:385234/1‑150 (MQ=255)
                                                                                                              cTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATg                                    >  2:1238667/1‑150 (MQ=255)
                                                                                                              cTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATg                                    >  2:3988596/1‑150 (MQ=255)
                                                                                                               tGTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGt                                   >  1:1155087/1‑150 (MQ=255)
                                                                                                                gTCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGtt                                  >  2:4265283/1‑150 (MQ=255)
                                                                                                                 tCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttc                                 >  1:4055487/1‑150 (MQ=255)
                                                                                                                 tCACCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttc                                 >  2:3209438/1‑150 (MQ=255)
                                                                                                                   aCCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttctt                               >  1:834930/1‑150 (MQ=255)
                                                                                                                   aCCGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttctt                               >  2:1206188/1‑150 (MQ=255)
                                                                                                                    ccGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttcttc                              <  1:5307320/150‑1 (MQ=255)
                                                                                                                    ccGAACTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGttcttc                              <  2:358343/150‑1 (MQ=255)
                                                                                                                       aaCTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGGTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTtcatc                           >  2:3473914/1‑150 (MQ=255)
                                                                                                                        aCTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTtcatca                          <  1:747413/150‑1 (MQ=255)
                                                                                                                        aCTGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTtcatca                          <  1:4667227/150‑1 (MQ=255)
                                                                                                                          tGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGc                        >  1:3815374/1‑150 (MQ=255)
                                                                                                                          tGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGc                        >  1:284548/1‑150 (MQ=255)
                                                                                                                          tGGATGATCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGc                        >  1:226893/1‑150 (MQ=255)
                                                                                                                              tgatCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAAcc                    <  1:4584201/150‑1 (MQ=255)
                                                                                                                               gatCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCg                   >  2:2542642/1‑150 (MQ=255)
                                                                                                                               gatCAACAAGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCg                   >  1:4036080/1‑150 (MQ=255)
                                                                                                                                  caacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTgg                <  1:1003750/150‑1 (MQ=255)
                                                                                                                                   aacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGa                                        <  2:2296406/125‑1 (MQ=255)
                                                                                                                                   aacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGc               >  1:2739740/1‑150 (MQ=255)
                                                                                                                                   aacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGc               >  1:2161328/1‑150 (MQ=255)
                                                                                                                                   aacaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGc               <  1:1027365/150‑1 (MQ=255)
                                                                                                                                    acaaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCt              >  2:3676668/1‑150 (MQ=255)
                                                                                                                                     caaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAAc                     <  2:2944800/142‑1 (MQ=255)
                                                                                                                                     caaGCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCtt             >  1:3897902/1‑150 (MQ=255)
                                                                                                                                        gCTGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGaa          >  2:1832429/1‑150 (MQ=255)
                                                                                                                                          tGGCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACa        >  1:3677459/1‑150 (MQ=255)
                                                                                                                                           ggCGACCGGCTATGTGGAAACCTTCCGCAACAGTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACAt       <  2:4768054/150‑1 (MQ=255)
                                                                                                                                            gCGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATg      <  1:2542642/150‑1 (MQ=255)
                                                                                                                                             cGACCGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATGc     <  2:468292/150‑1 (MQ=255)
                                                                                                                                                ccGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATGCCTg  <  1:3594231/150‑1 (MQ=255)
                                                                                                                                                ccGGCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATGCCTg  <  1:2803718/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGACAGTTATGCGCGGGTGTTCGTCATCGGCCTGCTCAATACGCTGTTGGTGACCTTCATCGGCGTCATTCTGGCGACGATACTGGGCTTCATCATCGGTGTGGCTCGCCTGTCACCGAACTGGATGATCAACAAGCTGGCGACCGTCTATGTGGAAACCTTCCGCAACATTCCGCCGTTGTTGCAGATCCTGTTCTGGTATTTCGCCGTGTTCCTGACCCTGCCGGGACCGCGGGGCAGCATCAACATCGAGGACATGTTCTTCATCAGCAACCGTGGCTTGAACATGCCTG  >  NC_002947/1484156‑1484449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑