| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 56,296 | 0 | T | . | 25.0% | 37.7 / 10.8 | 16 | coding (1332/1467 nt) | PP_0049 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/10); new base . (4/0); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGGACTGGCTGCTGTCAGCAACTCATCGAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTCAGCAGACCACGGCTCGTAGCCATATTGCGGCCAGAGCTGACCGTAGT > NC_002947/56172‑56423 | gaggaCTGGCTGCTGTCAGCAACTCATCGAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGTGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTg < 2:775359/136‑1 (MQ=255) ggCTGCTGGAAGAAACTCATCTAGTGACGGAACGTTCGGGTGTGCACTGAGGACGAGTGTTCTGGATCGTAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACt < 1:389040/136‑1 (MQ=255) aTCGAGTGACGTAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTa < 1:451478/136‑1 (MQ=255) aTCGAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTa < 1:102217/136‑1 (MQ=255) aTCGAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTa > 1:178516/1‑136 (MQ=255) gAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGc < 1:373983/136‑1 (MQ=255) gACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCg > 2:600119/1‑136 (MQ=255) ggAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGTATACATCAAATAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGt < 2:797893/136‑1 (MQ=255) ttAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTg < 1:236697/136‑1 (MQ=255) gACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAAt < 1:207015/136‑1 (MQ=255) gACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAAt < 1:440501/136‑1 (MQ=255) tGGATCGGAGTGTGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTA‑‑‑CTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTcagc > 2:661470/1‑136 (MQ=255) tGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTA‑‑‑CTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTcagc > 2:210608/1‑136 (MQ=255) tGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTA‑‑‑CTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTcagc > 2:806177/1‑136 (MQ=255) tGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTA‑‑‑CTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGCTATAGTGCCATAGGGTTATGGAATACCTCCACTTcagc > 2:665258/1‑136 (MQ=255) ccGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTCAGCAGACCACGGCTCGTAGCCATATTGCGGCCAGAGCTGACCGTAGt < 1:110343/136‑1 (MQ=255) | GAGGACTGGCTGCTGTCAGCAACTCATCGAGTGACGGAACCTTAGCGTTTGCACTGAGGACGAGTGTTCTGGATCGGAGTATGGATACATCAAAGAAAGATTCGCACTCGACTCTTCCGTTTACTTCTCTCCAGTGGGTGACTTCTGGTATCAACTCATTAGGCACCGGGTATAGTGCCATAGGGTTATGGAATACCTCCACTTCAGCAGACCACGGCTCGTAGCCATATTGCGGCCAGAGCTGACCGTAGT > NC_002947/56172‑56423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |