Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,992,8810AC37.1% 41.0 / 11.2 35F238V (TTC→GTC) PP_3517hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/8);  new base C (0/13);  total (14/21)
Fisher's exact test for biased strand distribution p-value = 1.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGCGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGCC  >  NC_002947/3992747‑3993013
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gAAGCGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAg                                                                                                                                     >  1:476842/1‑136 (MQ=255)
  aGCGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGcc                                                                                                                                   >  2:794063/1‑136 (MQ=255)
   gCGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCTTGAAGCTCATCACCCGGGTGTCGTCGTAGTTGGCTGG‑CCAGGCGACCATGGCGAAGCCa                                                                                                                                  <  1:204408/136‑1 (MQ=255)
    cGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCAc                                                                                                                                 >  2:444825/1‑136 (MQ=255)
     ttttCATCTCCTGCCCCCCCCTGGCGCCCTGCGGCCCGAGGTCTTTCTCCAACACTTGGCCGTCAGGGCTGATCATGAACCACATCACCCCGGTGGCGTCGAACTGGGCCGG‑CCCGGCGACCAGGGCTACGCCACg                                                                                                                                <  2:584286/135‑1 (MQ=255)
           tctcCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCAt                                                                                                                          >  1:625030/1‑136 (MQ=255)
           tctcCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCAt                                                                                                                          >  1:578948/1‑136 (MQ=255)
                    ccAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCCGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGcc                                                                                                                 <  2:476842/136‑1 (MQ=255)
                     cAGACTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGGTGCCg                                                                                                                >  2:445895/1‑136 (MQ=255)
                        ccTGTCGCCTTGCGGCCGGTGGTCTTTCTCTAACACTTGGCGGTCTTGGCTGATCTTGAAGCTCATCACCCGGGTGGGGTGTTACTTGGCGGG‑CCAGGCTACCTTGGCGACCCCACGGCTCATCTGGTTGCCGATc                                                                                                             <  2:457670/136‑1 (MQ=255)
                                              ccTTTCTCTAACACTTGCCCTTCTTGGCTTATAATCACCCTCTCCCCCCGGGTGTCGGCTTAGTTGGCCCG‑CCCGGCTACCTGGGCCAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCg                                                                                       <  2:814367/135‑1 (MQ=255)
                                                        acacTTGGCCGTCATGGCTGACCAGCACGCCCACCCCCCCGGGGCCGTCGAACTTGGCCGG‑CCCGGCGACCTGGGCCACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATgg                                                                             <  2:525919/136‑1 (MQ=255)
                                                                 cGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGc                                                                    >  2:415253/1‑136 (MQ=255)
                                                                                          caCCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATc                                                                                                             >  1:550298/1‑70 (MQ=255)
                                                                                          caCCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATc                                                                                                             <  2:550298/70‑1 (MQ=255)
                                                                                           aCCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCAt                                          >  2:279622/1‑136 (MQ=255)
                                                                                                ggTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAg                                     <  1:368984/136‑1 (MQ=255)
                                                                                                  tgtCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCAGTGCATACAGGATGCGGAAGTGGTAGCCATACCAgcc                                   >  1:649173/1‑134 (MQ=255)
                                                                                                    tcgtcgTACTTGGCCGG‑CCAGGCGACCTTGGCCACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                 <  1:250007/136‑1 (MQ=255)
                                                                                                    gggtcgtAC‑TGGCCGGCCCCGGCGACCAGGGCTACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                 <  1:833035/134‑1 (MQ=255)
                                                                                                    gcgtcgtACTGGGCCGG‑CCCGGCGACCTTGGCGACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                 <  1:245471/135‑1 (MQ=255)
                                                                                                     cgtcgtACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTc                                >  2:284478/1‑136 (MQ=255)
                                                                                                         gtACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCg                            >  2:53939/1‑136 (MQ=255)
                                                                                                          tACTTGGCAGG‑CCAGGCGACCATGGCGAAGCCACTGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGTGCCCAGTGCATCCATGATGCGGAAGTGTTAGCCATACCAGGCGTCACCGa                           >  2:208674/1‑136 (MQ=255)
                                                                                                             ttGGCCGC‑CCCGGCCACCTGGGCCACGCCACGGCTCATCTGGTTGCTGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACca                        <  1:453410/136‑1 (MQ=255)
                                                                                                               ggccgg‑ccCGGCGACCATGGCGACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACcaca                      <  2:578948/136‑1 (MQ=255)
                                                                                                               ggccgg‑ccCGGCGACCATGCCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACcaca                      <  2:193401/136‑1 (MQ=255)
                                                                                                               ggccgc‑ccCGGCCACCATGCCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACcaca                      <  2:625030/136‑1 (MQ=255)
                                                                                                                 ccgc‑ccAGGCGACCAGGCCCACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATc                    <  2:64911/136‑1 (MQ=255)
                                                                                                                  cgg‑ccAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATcc                   >  2:554391/1‑136 (MQ=255)
                                                                                                                           ctacgttGGCTACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGaa           <  1:60353/130‑1 (MQ=255)
                                                                                                                             ccctgggCGACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACa         <  1:75364/131‑1 (MQ=255)
                                                                                                                                   gCGACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGc   <  1:444825/136‑1 (MQ=255)
                                                                                                                                   gCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGc   <  1:415253/136‑1 (MQ=255)
                                                                                                                                    cGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGcc  <  1:236215/136‑1 (MQ=255)
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GAAGCGTTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGG‑CCAGGCGACCATGGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGCC  >  NC_002947/3992747‑3993013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑