| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,231,161 | 0 | G | C | 38.1% | 7.1 / 12.1 | 21 | intergenic (‑133/+43) | PP_4606/PP_4607 | ferric siderophore receptor/transmembrane sensor |
| Reads supporting (aligned to +/- strand): ref base G (2/11); new base C (8/0); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTC > NC_002947/5231026‑5231290 | cATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑AGG‑TAAACCCg < 1:471048/136‑1 (MQ=255) gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TTAACcccccc > 1:441642/1‑136 (MQ=255) gCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TAc < 2:630515/136‑1 (MQ=255) gtgAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACGGGGGGtt < 2:19636/136‑1 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAAACCCCCCA‑ACCGGGGGGTTTACCCggaaa > 1:836983/1‑132 (MQ=255) acaatcACAGTTTCACTCATTACACGGATGCCTCGGGCTGTTAGCTCACGTGCGCCTGGAATTATTTTTGCTGGGGCGGCCTCTTCGG‑GGG‑AAACCCCGCCCC‑TAGGGGGGGTTTACCCGTGAAGTGGCTGGcaca < 2:634890/135‑1 (MQ=255) caatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TTAACCCCCCCC‑TAGGGGGGGTTTA‑GCGTGTAGAGGGTggggcag > 1:169802/1‑131 (MQ=255) caatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACggggg > 2:697019/1‑110 (MQ=255) caatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACggggg < 1:697019/110‑1 (MQ=255) cGCACTTTCACTCATTACACGGATGCCACGTGCTGTTCCCTCACCTGTGCCTGTAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TCCGGGGGGTTAACCGGTGAAGAGGCTGGCACAGGCaa < 2:623008/136‑1 (MQ=39) tttCAGTCATTACCGCGATGCCTCGGGGGGATACCTCCCGTGTGCCTGAATATATTTTTGCTGTGGGGGCCTATTCGC‑GGG‑TAAACCCGCCCC‑TCGGGGGGGTTTACCGGTGATGAGGGTGGCACAGGCAACTAcc < 2:701019/136‑1 (MQ=255) aaagggATGCCACGTGCTGTTACGTCACCTGTGGCTGTAATTATTTTTGCTGGGGCGGCCTCTTCGG‑GGG‑AAACCCCGCCCC‑TAGGGGGGGTTTACCCGTGATGTGGCGGGCAAAGGCAACTACCGAACCAGGGTc < 2:723231/132‑1 (MQ=255) gCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCCCCCCATACGGGGGGTTT‑GCCGTGTATTTGGTTGGACAGGCAAAAACCGCACCAGGGGGAAACGccc > 1:213589/1‑136 (MQ=255) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCG‑AAGGGGGGGTTTACCC‑TGTAGAGGCTGGGCCAGGCAACAAACGCACCACGGGCAAACGccccc > 1:158548/1‑135 (MQ=255) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCCCCCC‑AACGGGGGGGTTTCCCCGGAAGGGGCGGGCACCGGCAACGAACGCACCCCGGGGAAAAGccccc > 1:61141/1‑135 (MQ=255) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAAACCCCCCC‑AACGGGGGGGTTTACCGTGTAGAGGCTGGCACAGGCAACAACCCCACCACGGTCAAAccccccc > 2:545634/1‑135 (MQ=255) cgCTGTTACCCCACCTGTGCCTGCAAATATTTTTGCTGTGGCGCCCGCTGCGC‑GGG‑TAACCCCGCCCA‑AAGGGGGGGTTAACCCGTTAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑Aacac < 1:635350/135‑1 (MQ=255) cTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAACCCCCCCCC‑AAGGGGGGGTTTACCCG‑GAAGAGGCTGGCACAGGCCACTAACCAACCACGGGCAAACGCCCC‑AACACCGTCTGCCGTGaaa > 2:185887/1‑136 (MQ=255) gtgCCTGTAAATATTTTTGCTGGGGCGGCCTCTGCGG‑GGT‑TAACCCCGCCCC‑TCCGGGGGGTTTACCCGTTAAGAGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGaaaaa < 1:763679/136‑1 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TAAACCCCCCCC‑ACCGGGGGGTTTACCCG‑GGAGAGGGGGGGGCACGCCACAACCCCACCCGGGGGAAACCCCCCAAACCCGGCTGGCCGGGAAAAAccc > 2:449264/1‑136 (MQ=255) ggAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGT‑TAAACCCGCCCC‑TACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGAAAAACCCATc < 2:638605/136‑1 (MQ=255) aaTATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGAAAAACCCATCAcc > 2:550475/1‑136 (MQ=255) aaccccGCCCC‑TCCGGGGGGTTAACCGGTTAATTGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTc < 1:441862/133‑1 (MQ=255) | CATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TACGGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCC‑AACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTC > NC_002947/5231026‑5231290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |