Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,367,766	A→G	F254L (TTC→CTC)	ftsH ←	integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,367,766	0	A	G	100.0%	71.0 / NA	20	F254L (TTC→CTC)	ftsH	integral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

ATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCG  >  NC_002947/5367636‑5367894                                                                                                                                   |                                                                                                                                 atgatgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCAACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGgatga                                                                                                                             <  2:637746/136‑1 (MQ=255)    atgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGc                                                                                                                          <  1:294289/136‑1 (MQ=255)    atgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGc                                                                                                                          <  1:417376/136‑1 (MQ=255)    atgCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGc                                                                                                                          >  2:280790/1‑136 (MQ=255)        catcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACgg                                                                                                                      >  2:533811/1‑136 (MQ=255)        catcatTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACgg                                                                                                                      >  2:31415/1‑136 (MQ=255)                    cGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGcttctt                                                                                                          <  1:214099/136‑1 (MQ=255)                     gAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGcttctt                                                                                                          <  1:409886/135‑1 (MQ=255)                     gAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGcttctt                                                                                                          >  2:409886/1‑135 (MQ=255)                       aGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGc                                                                                                       <  1:801519/136‑1 (MQ=255)                       aGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGc                                                                                                       <  1:65346/136‑1 (MQ=255)                                             cTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGcacc                                                                                 >  1:196042/1‑135 (MQ=255)                                                 ttGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGc                                                                             >  2:781935/1‑136 (MQ=255)                                                             tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                                                 >  2:164861/1‑136 (MQ=255)                                                             tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                                                 >  2:694442/1‑136 (MQ=255)                                                                                  cccATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTAGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGGTGGCACCGCCACCCACGAACAATTACAAGAAGt                                            >  2:389701/1‑136 (MQ=255)                                                                                                aCGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATg                              <  1:533811/136‑1 (MQ=255)                                                                                                aCGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATg                              <  1:31415/136‑1 (MQ=255)                                                                                                    tGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTga                          >  1:397456/1‑136 (MQ=255)                                                                                                                            gcgATGAGGATGATGCACGGGGCGTGCTTCTTTGCCTGCTCGAACATGTCGCGCACTCGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCcg  <  1:646421/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 ATGATGCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTTCGCCCG  >  NC_002947/5367636‑5367894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑