Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,827,925	G→A	S29F (TCC→TTC)	ptxS ←	2‑ketogluconate utilization repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,827,925	0	G	A	100.0%	86.3 / NA	25	S29F (TCC→TTC)	ptxS	2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/18);  total (7/18)

CCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTT  >  NC_002947/3827808‑3828052                                                                                                                      |                                                                                                                                ccGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGaaa                                                                                                               <  1:304877/136‑1 (MQ=255)    cgcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGc                                                                                                            <  1:200667/136‑1 (MQ=255)        ttAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGc                                                                                                        >  2:148719/1‑136 (MQ=255)             ccGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGAGGCTTTGGAAACGCCAGCAacac                                                                                                   >  1:24025/1‑136 (MQ=255)                 gAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACgcgc                                                                                               <  1:452352/136‑1 (MQ=255)                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                             >  1:132247/1‑136 (MQ=255)                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                             >  2:606945/1‑136 (MQ=255)                      aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACtt                                                                                          <  2:820975/136‑1 (MQ=255)                      aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACtt                                                                                          <  2:639792/136‑1 (MQ=255)                      aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACtt                                                                                          <  1:724422/136‑1 (MQ=255)                      aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACtt                                                                                          <  2:626923/136‑1 (MQ=255)                                   gATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAc                                                                             <  2:432097/136‑1 (MQ=255)                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                         <  1:290714/136‑1 (MQ=255)                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                         <  1:618099/136‑1 (MQ=255)                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                         <  2:474052/136‑1 (MQ=255)                                        ccGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGtt                                                                        >  1:571184/1‑136 (MQ=255)                                                          tctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCATTTGGCGGTCTCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgcg                                                      <  2:161198/136‑1 (MQ=255)                                                              ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTc                                                  <  1:283514/136‑1 (MQ=255)                                                               cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCg                                                 <  1:349469/136‑1 (MQ=255)                                                                         gCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGtt                                       >  2:651869/1‑136 (MQ=255)                                                                                 ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                               <  2:552092/136‑1 (MQ=255)                                                                                       cAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGt                         >  2:285104/1‑136 (MQ=255)                                                                                                      gCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaa          <  2:525250/136‑1 (MQ=255)                                                                                                           cGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCgg     <  2:571184/136‑1 (MQ=255)                                                                                                              tGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt  <  1:247004/136‑1 (MQ=255)                                                                                                                      |                                                                                                                                CCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTT  >  NC_002947/3827808‑3828052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑