Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,674,240	G→A	50.0%	R109H (CGC→CAC)	PP_3235 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,674,240	0	G	A	50.0%	‑6.3 / 6.8	8	R109H (CGC→CAC)	PP_3235	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (2/2);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAACGAGGACCTGTTCGATGTCTTCCTCAATATGCGGCGCGCCAATATCCGTCGGCACATCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCGTTGACCTCATGG  >  NC_002947/3674174‑3674365                                                                   |                                                                                                                              aGAACGAGGACCTGTTCGATGTCTTCCTCAATATGCGGCGCGCCAATATCCGTCGGCACATCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGAtt                                                          >  1:84781/1‑136 (MQ=255)                         tCCTCAATATGCGGCGCGCCAATATCCGTCGGCACATCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGAcgtgcg                                  <  1:131387/136‑1 (MQ=255)                                     ggcgCGCCAATATCCGTCGGCACATCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGggtggt                      <  1:25746/136‑1 (MQ=255)                                             aaTATCCGTCGGCACATCGCTCACACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCg              >  2:224259/1‑136 (MQ=255)                                             aaTATCCGTCGGCACATCGCTCACACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCg              >  2:225583/1‑136 (MQ=255)                                                      cGGCACATCGCTCACACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCGTTGACCTCa     <  1:224259/136‑1 (MQ=255)                                                      cGGCACATCGCTCACACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCGTTGACCTCa     <  1:225583/136‑1 (MQ=255)                                                         cacaTCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCGTTGACCTCATgg  >  1:143572/1‑136 (MQ=255)                                                                   |                                                                                                                              AGAACGAGGACCTGTTCGATGTCTTCCTCAATATGCGGCGCGCCAATATCCGTCGGCACATCGCTCGCACAGGCAAGCACCACCTCGGGCATCTCGTCATCATGGCAGGCCCAGCCCCAGCGCCCGATACCTGATTGGCAATGCAGGCTCCAGACGTGCGTGTGAGGGTGGTGAATGTCGTTGACCTCATGG  >  NC_002947/3674174‑3674365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑