| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,136,737 | G→T | 25.9% | P87T (CCC→ACC) | PP_3638 ← | acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,136,737 | 0 | G | T | 25.9% | 29.7 / 4.0 | 15 | P87T (CCC→ACC) | PP_3638 | acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (8/3); new base T (2/2); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTGGG > NC_002947/4136611‑4136870 | ggcggcCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGTGCTGTgcag > 1:12424/1‑136 (MQ=255)ggcggcCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGTGCTGTgcag > 1:11096/1‑136 (MQ=255) gcgcTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGc > 2:188503/1‑136 (MQ=255) cGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTg > 2:205262/1‑136 (MQ=255) aTACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGc > 2:239061/1‑136 (MQ=255) aGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGa > 1:256223/1‑136 (MQ=255) ttCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAg < 1:31414/136‑1 (MQ=255) ggTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTg > 2:159862/1‑136 (MQ=255) ggTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTg > 2:142345/1‑136 (MQ=255) gCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATggcgg > 1:164422/1‑136 (MQ=255) cagcagGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCAcc > 2:250631/1‑136 (MQ=255) gCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTAcc < 1:159862/136‑1 (MQ=255) gCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTAcc < 1:142345/136‑1 (MQ=255) ttGGTGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTg < 2:12424/136‑1 (MQ=255) ttGGTGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTg < 2:11096/136‑1 (MQ=255) ggggCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTggg > 2:162068/1‑136 (MQ=255) ggggCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTggg > 1:144870/1‑136 (MQ=255) ggggCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGCTCACCTGCCCGCCCGTACCGCCCAGCGCTggg > 2:213251/1‑136 (MQ=255) | GGCGGCCACGCACCTGCAGCGCTTCGATACCGGACAGGAACTTCATGGCGTTGGACAGCCCGGTGGCACCGGCCAATTCACCGGTCAACAGCCGCGGCAGCAGGCGCTCACGCAGGGGCTGGTTGGGGCTGTGCAGCAGGTATTCGATGAAGGCACGCTGGCCCCAGCAGACGAAAGCCGCGGCCAATGAGCGGCTGGCAATGGCGGCGATGGCCTCGACCGCGTCGGTCACCTGCCCGCCCGTACCGCCCAGCGCTGGG > NC_002947/4136611‑4136870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |