| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,551,306 | C→T | 30.8% | A26V (GCC→GTC) | pydX → | NADP‑dependent dihydropyrimidine dehydrogenase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,551,306 | 0 | C | T | 30.8% | 11.7 / 5.9 | 13 | A26V (GCC→GTC) | pydX | NADP‑dependent dihydropyrimidine dehydrogenase subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base T (2/2); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01 | |||||||||||
CGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCAACGCTTGCCCCAGCGAAATAGACATTCC > NC_002947/4551184‑4551408 | cGCAAAACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTACAGCGACCTGGCCCCACCCCACAcc > 1:72391/1‑136 (MQ=255) ccTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTc < 2:20530/136‑1 (MQ=255) cTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGTCCCACCCCTCACCGCGCGTCAGgccgcc > 2:202818/1‑136 (MQ=255) cTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGTCCCACCCCTCACCGCGCGTCAGgccgcc > 2:41477/1‑136 (MQ=255) cTGCACC‑AAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCCACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGccc < 2:185332/136‑1 (MQ=255) aaaTAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGTCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCa < 2:180609/136‑1 (MQ=255) atACAACCGTGACCAACGCCCTGAACCTTTTGCCGCGCCCCCGGGCCGGGGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTa < 2:31881/135‑1 (MQ=255) ccAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCCCCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGc > 1:217329/1‑136 (MQ=255) cATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCCCCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCGGTACTGCTACGACGCCCCCTGCGTCAAcc > 1:216442/1‑135 (MQ=255) cccccGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCATCGCTTGCCCCAGc < 1:90656/136‑1 (MQ=255) gcgcCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGTCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCAACGCTTGCCCCAGCGAAATAGACAt < 1:202818/136‑1 (MQ=255) gcgcCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGTCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCAACGCTTGCCCCAGCGAAATAGACAt < 1:41477/136‑1 (MQ=255) ccGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCAACGCTTGCCCCAGCGAAATAGACATTcc > 2:51182/1‑136 (MQ=255) | CGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCTGGCCCCACCCCTCACCGCGCGTCAGGCCGCCCTGGAAAGTGCACGCTGCCTGTACTGCTACGACGCCCCCTGCGTCAACGCTTGCCCCAGCGAAATAGACATTCC > NC_002947/4551184‑4551408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |