| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,759,486 | C→A | 11.8% | T264T (ACC→ACA) | ompQ → | outer membrane pyoverdine efflux protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,759,486 | 0 | C | A | 11.8% | 108.5 / 5.6 | 34 | T264T (ACC→ACA) | ompQ | outer membrane pyoverdine efflux protein |
| Reads supporting (aligned to +/- strand): ref base C (19/11); new base A (2/2); total (21/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
AGCTGGCCCAGCAGCGCAGCCTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAGCGCCAATGTGCAGGTCGCCAGGGCA > NC_002947/4759353‑4759621 | aGCTGGCCCAGCAGCGCAGCCTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCgc < 2:248177/136‑1 (MQ=255) gCCCAGCAGCGCAGCCTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCAt > 1:151404/1‑136 (MQ=255) gcCTGGTGGCCGCTCAGCTTCGCCTACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACGCTGGCAACGCTACTGGGGCTACCGGTGCAACTACTGCCGACCAGTACCGCAGCATTCGCTGACCTgcg < 2:129034/136‑1 (MQ=255) gcCTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTgcg < 2:253767/136‑1 (MQ=255) ccTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCACTCGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTgcgc > 2:51694/1‑136 (MQ=255) ggtggCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTgg > 1:178532/1‑136 (MQ=255) ggCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCt < 2:151404/136‑1 (MQ=255) cAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCg > 1:219879/1‑136 (MQ=255) aCGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGt < 2:214513/136‑1 (MQ=255) aCGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGt < 2:178532/136‑1 (MQ=255) aCTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGt > 2:165951/1‑130 (MQ=255) aCTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGt < 1:165951/130‑1 (MQ=255) aCTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAg > 1:114359/1‑136 (MQ=255) ggCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCgc < 2:156540/70‑1 (MQ=255) ggCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCgc > 1:156540/1‑70 (MQ=255) cccAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACAt < 1:94488/136‑1 (MQ=255) cACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAgcc > 1:154946/1‑136 (MQ=255) cTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGccgccg > 2:245379/1‑136 (MQ=255) aCGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACAGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGa < 1:233492/116‑1 (MQ=255) aCGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACAGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGa < 1:232203/116‑1 (MQ=255) aCGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACAGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGa > 2:233492/1‑116 (MQ=255) aCGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACAGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGa > 2:232203/1‑116 (MQ=255) cGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGgcgcg > 2:231609/1‑136 (MQ=255) cGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGgcgcg > 2:230612/1‑136 (MQ=255) tACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGccg > 1:54703/1‑135 (MQ=255) cTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTgg > 2:18013/1‑136 (MQ=255) cTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTgg > 1:172450/1‑136 (MQ=255) ggCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCGCGACATCGCAGCCGCCGAGGCGCGCCTGGCcgc < 1:51694/136‑1 (MQ=255) ggCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCcgc > 2:31754/1‑136 (MQ=255) aCCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGcc > 2:267323/1‑136 (MQ=255) ggTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAgc > 2:20163/1‑136 (MQ=255) ggTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAgc > 2:244572/1‑136 (MQ=255) ggTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAgc > 2:161513/1‑136 (MQ=255) cGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAGCGCCAATGTGCAGGt < 2:226019/136‑1 (MQ=255) cGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAGCGCCAATGTGCAGGTCGCCAGGGcc > 1:23183/1‑135 (MQ=255) | AGCTGGCCCAGCAGCGCAGCCTGGTGGCCGCTCAGCAACGCCAACTGCCGCTACTTGAGCAGCAGTGGCAAGACACCCAGGTCACCCTGGCAACGCTACTGGGGCTACCGGTGCAACAACTGCCGACCAGTACCGCAGCATTCGCTGACCTGCGCTGGCCTCGCATCGCCAGTGGCGTACCCAGTGAGCTGCTGGCGCGTCGCCCCGACATCGCAGCCGCCGAGGCGCGCCTGGCCGCGGCCAGCGCCAATGTGCAGGTCGCCAGGGCA > NC_002947/4759353‑4759621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |