Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,720,671 G→A 10.8% Q123Q (CAG→CAA)  PP_1516 → RND membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,720,6710GA10.8% 107.6 / 3.8 37Q123Q (CAG→CAA) PP_1516RND membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (9/24);  new base A (2/2);  total (11/26)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

GCGGGTCAAGGCCGACCAGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAACCAGGCCG  >  NC_002947/1720536‑1720795
                                                                                                                                       |                                                                                                                            
gCGGGTCAAGGCCGACCAGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAg                                                                                                                              >  2:190207/1‑136 (MQ=255)
                 aGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCa                                                                                                             >  2:222299/1‑136 (MQ=255)
                 aGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCa                                                                                                             >  2:19061/1‑136 (MQ=255)
                 aGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCa                                                                                                             >  2:11002/1‑136 (MQ=255)
                 aGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCa                                                                                                             >  1:84820/1‑136 (MQ=255)
                  gCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAg                                                                                                            >  2:198878/1‑136 (MQ=255)
                            caaCTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCACCCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGcc                                                                                                  <  2:97512/135‑1 (MQ=255)
                              aCTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCAc                                                                                                <  2:221992/136‑1 (MQ=255)
                                              gTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATg                                                                                <  2:44165/136‑1 (MQ=255)
                                                  gcCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGa                                                                            >  2:255759/1‑136 (MQ=255)
                                                  gcCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGa                                                                            >  1:58905/1‑136 (MQ=255)
                                                   ccTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGaa                                                                           <  2:67927/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGGGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  1:167203/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  1:102032/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  2:96607/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  2:48537/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  1:123298/136‑1 (MQ=255)
                                                        aaCTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACct                                                                      <  1:47088/136‑1 (MQ=255)
                                                                 cgaaCCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGc                                                             <  2:107509/134‑1 (MQ=255)
                                                                       gcgcCCTGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCt                                                       <  1:248100/136‑1 (MQ=255)
                                                                       gcgcCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCt                                                       <  2:102857/136‑1 (MQ=255)
                                                                       gcgcCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCt                                                       <  2:58905/136‑1 (MQ=255)
                                                                       gcgcCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCt                                                       >  2:204942/1‑136 (MQ=255)
                                                                                 ggcggcggcCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTg                                             <  1:123553/136‑1 (MQ=255)
                                                                                 ggcggcTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTg                                             <  2:206792/136‑1 (MQ=255)
                                                                                 ggcggcTGCCGAGGCCAACCTGGCATTGGTGAGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTg                                             <  1:266150/136‑1 (MQ=255)
                                                                                             ggCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAg                                 <  1:222299/136‑1 (MQ=255)
                                                                                             ggCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAg                                 <  1:19061/136‑1 (MQ=255)
                                                                                               ccAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGc                               <  2:150937/136‑1 (MQ=255)
                                                                                                                cgcgcCGAACGCGACCGCTACCAAAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGaa                                                                           <  1:128087/75‑1 (MQ=255)
                                                                                                                cgcgcCGAACGCGACCGCTACCAAAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGaa                                                                           >  2:2867/1‑75 (MQ=255)
                                                                                                                cgcgcCGAACGCGACCGCTACCAAAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGaa                                                                           <  1:2867/75‑1 (MQ=255)
                                                                                                                cgcgcCGAACGCGACCGCTACCAAAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGaa                                                                           >  2:128087/1‑75 (MQ=255)
                                                                                                                  cgcCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGc            <  1:210003/136‑1 (MQ=255)
                                                                                                                      gAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAAcc        <  1:255759/136‑1 (MQ=255)
                                                                                                                            gACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAACCAGGCCg  <  1:4982/136‑1 (MQ=255)
                                                                                                                                gCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAACCAGGCCg  <  1:30551/132‑1 (MQ=255)
                                                                                                                                gCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAACCAGGCCg  >  2:30551/1‑132 (MQ=255)
                                                                                                                                       |                                                                                                                            
GCGGGTCAAGGCCGACCAGCCGCTGGCCGAACTGGACCCGCAGGACGTGCGCCTGCAACTTGAAGCCAACCGCGCCCAGCTGGCGGCTGCCGAGGCCAACCTGGCATTGGTGCGCGCCGAACGCGACCGCTACCAGAAGCTGCTGGAGCGGCAGATGGTCAGCCACTCCCAGTACGACAATGCCGAAAACCTCTACCGTGCAGGCCTTGCCCGTCTGAAGCAGGCCAAGGCCGAGTTCGATGTGGCGGGCAACCAGGCCG  >  NC_002947/1720536‑1720795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑