breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | JBEI_Xylose_A28_F48_I0_S29_R1_001.good.fq | 271,842 | 36,193,677 | 100.0% | 133.1 bases | 136 bases | 86.6% |
errors | JBEI_Xylose_A28_F48_I0_S29_R2_001.good.fq | 271,842 | 36,193,677 | 100.0% | 133.1 bases | 136 bases | 74.0% |
total | 543,684 | 72,387,354 | 100.0% | 133.1 bases | 136 bases | 80.3% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_002947 | 6,183,858 | 9.2 | 5.7 | 100.0% | na |
total | 6,183,858 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 910 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 52 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.002 |
reference sequence | pr(no read start) |
---|---|
NC_002947 | 0.97123 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
option | value |
---|---|
Mode | Full Polymorphism |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 2 |
Polymorphism frequency cutoff | 0.05 |
Polymorphism minimum variant coverage each strand | 2 |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 23:30:21 17 Jul 2020 | 23:30:37 17 Jul 2020 | 16 seconds |
Read alignment to reference genome | 23:30:38 17 Jul 2020 | 23:31:52 17 Jul 2020 | 1 minute 14 seconds |
Preprocessing alignments for candidate junction identification | 23:31:52 17 Jul 2020 | 23:32:03 17 Jul 2020 | 11 seconds |
Preliminary analysis of coverage distribution | 23:32:03 17 Jul 2020 | 23:32:20 17 Jul 2020 | 17 seconds |
Identifying junction candidates | 23:32:20 17 Jul 2020 | 23:32:20 17 Jul 2020 | 0 seconds |
Re-alignment to junction candidates | 23:32:20 17 Jul 2020 | 23:32:36 17 Jul 2020 | 16 seconds |
Resolving best read alignments | 23:32:36 17 Jul 2020 | 23:32:55 17 Jul 2020 | 19 seconds |
Creating BAM files | 23:32:55 17 Jul 2020 | 23:33:08 17 Jul 2020 | 13 seconds |
Tabulating error counts | 23:33:08 17 Jul 2020 | 23:33:17 17 Jul 2020 | 9 seconds |
Re-calibrating base error rates | 23:33:17 17 Jul 2020 | 23:33:18 17 Jul 2020 | 1 second |
Examining read alignment evidence | 23:33:18 17 Jul 2020 | 23:36:21 17 Jul 2020 | 3 minutes 3 seconds |
Polymorphism statistics | 23:36:21 17 Jul 2020 | 23:36:26 17 Jul 2020 | 5 seconds |
Output | 23:36:26 17 Jul 2020 | 00:06:27 18 Jul 2020 | 30 minutes 1 second |
Total | 36 minutes 5 seconds |