Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,511,8780TG44.1% ‑1.4 / 10.7 36L36R (CTC→CGC) urtBurea ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (3/16);  new base G (8/7);  total (11/25)
Fisher's exact test for biased strand distribution p-value = 3.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCC  >  NC_002947/5511745‑5512011
                                                                                                                                     |                                                                                                                                     
ccAACCCCTATTTCCCGCATTGCCCGCACGCTCACACTCCTGCCCACCCTCCTGCTGCTACCCCCCCTCGCAACCCACGCCAGCGA‑GGCAAATTCTTTCTCACCCCCACCCCCGCCGCGCCGGCCGGCCGGCTCGa                                                                                                                                    <  2:945654/136‑1 (MQ=255)
           ttCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCCCGCCAGCGCGGGCGAATTCTTTCTCCCCCCCAAGCCCGCCGCGCAGGCCGGCCTGCTCGAAGGCTGGgca                                                                                                                          <  2:729936/136‑1 (MQ=255)
           ttCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGgca                                                                                                                          <  1:1039995/136‑1 (MQ=255)
           ttCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGCCACCCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGgca                                                                                                                          <  1:506062/136‑1 (MQ=255)
            tCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCGCGCAGGCGGGgcag                                                                                                                         >  1:27669/1‑136 (MQ=255)
                         gCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCCGCCCGCGCGAAGGCGGGGCCGCACAAccccaaac                                                                                                            >  1:455574/1‑131 (MQ=255)
                         gCATGCGCAGACTCCTCCTCACCCTCCTGCTTCTAACCCCCGTCGAAACCCACGCCTGCGTGGCCTATTTCTTTCACACCGCCACGCCCGCCGCGCAGGGCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGc                                                                                                            <  2:541275/136‑1 (MQ=255)
                                     cccTGCCCACCCTCGTGCTGCAAACCCCCCTCGCACCCCCCGCCAGCGCGCGCTAATTCTTCCCCCCCGCCACGCCCGCCGCGCCGGCGGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGcc                                                                                                <  1:567888/135‑1 (MQ=255)
                                          cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCGGGCCGGCCGGCGCGCAGGCGGGGCGGCCCAACCCCATGCCGCGCCCCAGCCgcggc                                                                                           >  1:297/1‑136 (MQ=255)
                                           ccACCCTCGTGCTGTAAACCCCCCTCCCAACCCACGCCCGCGCGGGCTAATTCTTTCACACCGCCACGCCCGCGGTGCAGGCCGGCCTGCCCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCgctgct                                                                                          <  2:123271/135‑1 (MQ=255)
                                              cccTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCCGGCCGGCCGGCGCCAAGGCGGGGCCGCACCACCCCCAGCCCCGCCCCGGCCGCGGCTggt                                                                                       >  2:1000751/1‑135 (MQ=255)
                                                  cctGCTGCTAATCCCCCTGGCAACCCACGCCAGCGATGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCCGCCGGGGCGAAAGCGGGGCCGCCCCACCCCCAGCCCCCCGCCAGCCCCAGCAGGAAAAc                                                                                   >  2:522237/1‑136 (MQ=255)
                                                     gctgctAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCGCGCAGGCGGGGCCGCCCCACCCCCAGCCCCGCGCCCGCCGCGGCGGGAAAAccaa                                                                                >  1:744235/1‑134 (MQ=255)
                                                            aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCCGGGCGGGCGGCGGGGGGGCGGGGCGGCCCCCCCCCCAGCCGCGCCCCGGCCGCGGCGGGAGAACCAGCGccccc                                                                         >  1:816904/1‑133 (MQ=255)
                                                            aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCGGGCCGGGCGGCGCGGAGGCGGGGCAGCCCACCCCCAAGCCGCGCGCCGGCCGCTGCGGGAAAACCAGCGCCaac                                                                         >  1:511975/1‑135 (MQ=255)
                                                                cccTGCCACCCCCCGCCCGCGAGGGCTAATTCTTACTCCCCCCCACGCCCGCCGAGCAGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCg                                                                     <  2:18553/136‑1 (MQ=255)
                                                                cccTGCCACCCCACCCCAGCGCGGGCTAATTCTTTCTCACCCCCACGCCCGCCGAGCAGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCg                                                                     <  2:275089/136‑1 (MQ=255)
                                                                   tcccacccccCGCCAGCGAGGGCTATTTCTTTCTCCCCGCCACGCCCGCCGCGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCAt                                                                  <  2:816904/130‑1 (MQ=255)
                                                                            ccGCCCGCGAGGGCTAATTCTTCCACCCCCCCACGCCCGCCGAGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCaa                                                         <  2:528827/135‑1 (MQ=255)
                                                                             gggcAGCGTGGGTGTATTCTTTCCCAGCGCCCAGCCGGCCGCGCTGGCCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAAt                                                        <  1:920185/133‑1 (MQ=255)
                                                                             cGCCCGCGTGTGCGTATTCTTCCTCCCCCGCACGCCCGCCGCCCCGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAAt                                                        <  1:607324/136‑1 (MQ=255)
                                                                              gCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGAAGGCTGGGCCGCACAACCCGCTGCCGCGCCCCAGCCGCGGCTGGGAAACCAGCGCCAAGGCCGCATCGCCCCCaaat                                                       >  2:271368/1‑134 (MQ=255)
                                                                                      gggCGAATTCTTTCTCACCGCCAAGCCCGCCGAGTAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAgcgc                                               >  1:507414/1‑136 (MQ=255)
                                                                                       ggttatttctttctCCCCGCCACCCCCGCCGCGCCGGCCGGGCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa                                              <  2:590759/130‑1 (MQ=255)
                                                                                       ggctatttctttcaCCCCCCCACCCCCGCCGCGCCGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa                                              <  2:216963/130‑1 (MQ=255)
                                                                                       ggCTAATTCTTTCTCACCCCCAAGCCCGCCGCGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa                                              <  2:1034822/136‑1 (MQ=255)
                                                                                         ctaaTTCTTCCACCCCCCCACCCCCGCCGCGCAGGCCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAATGCCGCATCGCCACCAATGACAAGCGCAAg                                            <  2:1093969/134‑1 (MQ=255)
                                                                                                             ccccccccGAGCCGGCCGGCCTGCCCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCt                        <  2:901430/134‑1 (MQ=255)
                                                                                                             cccccGCCGCGCCGGCCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCt                        <  2:895734/134‑1 (MQ=255)
                                                                                                               cccGCCGCCCCGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTgc                      <  1:895402/136‑1 (MQ=255)
                                                                                                                 cgccgcgccgGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTgcgc                    <  2:744235/136‑1 (MQ=255)
                                                                                                                      ccctggcgggccTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCt               <  2:762565/132‑1 (MQ=255)
                                                                                                                           gccggccTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCg          <  2:455574/136‑1 (MQ=255)
                                                                                                                                 gggCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACg    <  2:297/134‑1 (MQ=255)
                                                                                                                                  tGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGc   <  1:516608/136‑1 (MQ=255)
                                                                                                                                   gCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGcc  >  1:139968/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCC  >  NC_002947/5511745‑5512011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑