| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,013,252 | T→G | intergenic (+77/+54) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,252 | 0 | T | G | 83.3% | 10.1 / ‑5.5 | 6 | intergenic (+77/+54) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (5/0); total (6/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCG > NC_002947/1013117‑1013379 | ggcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGTGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 2:65446/135‑1 (MQ=255)cgcTTATGGAAGAGCGCTGGCCGGACATCATTTTCCGCGCTACCCGCGAGCACCATTATGGCCTGAATTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 1:810093/136‑1 (MQ=38)cgcTGATGGAAGCGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 2:104593/136‑1 (MQ=255) cTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt < 2:439410/136‑1 (MQ=255) cTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCACGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCAACAGGAATCACCAGACCTGGAGGTAAtgt < 2:966989/136‑1 (MQ=255) cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCCTTTCAATTTCCGGCGTTGATACTGGTACAGATTCGCCTACGAATACGGTCGCTCTTCCCCGGCATCGCCTACa > 1:85587/6‑136 (MQ=21) cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTGCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa > 1:351587/6‑136 (MQ=37) cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTccc > 1:360503/6‑135 (MQ=37) cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCGTTGATACTGGTCCAGATTCGCGTACGAATACCGTCGATCTTAGCCGGCATCGCCTccc > 1:828295/6‑135 (MQ=25) cctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTACGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTccc > 1:619062/6‑135 (MQ=37) ggAGGTCATGTGATACATGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTTGGCGGCCACATTTCAATTTCCGGCTTTGTTAATGGTCCAGATTAGCGTACGAATACGGTCGCTCTTCGCCGGCATCGCCTCCAgcg > 2:601776/1‑136 (MQ=255) | CGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCG > NC_002947/1013117‑1013379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |