Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,367,766	A→G	F254L (TTC→CTC)	ftsH ←	integral membrane ATP‑dependent zinc metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,367,766	0	A	G	100.0%	69.1 / NA	20	F254L (TTC→CTC)	ftsH	integral membrane ATP‑dependent zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

GCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTT  >  NC_002947/5367641‑5367888                                                                                                                              |                                                                                                                           gCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCAc                                                                                                                  <  1:1076979/136‑1 (MQ=255)     tcattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACgggg                                                                                                              <  1:320277/136‑1 (MQ=255)     tcattcatCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACgggg                                                                                                              <  2:1050756/136‑1 (MQ=255)              tcGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGcttct                                                                                                     >  1:11567/1‑136 (MQ=255)                    ccGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCt                                                                                               >  1:691219/1‑136 (MQ=255)                                        cTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGcacc                                                                           >  1:626948/1‑135 (MQ=255)                                              gAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTg                                                                     >  2:621612/1‑136 (MQ=255)                                                        tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                                           >  1:1160594/1‑136 (MQ=255)                                                        tCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGacac                                                           >  1:644038/1‑136 (MQ=255)                                                              tcgtcgTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGa                                                     <  2:691219/136‑1 (MQ=255)                                                                         gccgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACggg                                                                                                               >  1:781459/1‑67 (MQ=255)                                                                         gccgccCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACggg                                                                                                               <  2:781459/67‑1 (MQ=255)                                                                                     ggCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCg                              <  2:548010/136‑1 (MQ=255)                                                                                                  cGACCAACGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTgaaga                 >  1:19986/1‑136 (MQ=255)                                                                                                       aaCGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGt            <  2:1045204/136‑1 (MQ=255)                                                                                                       aaCGGCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGt            <  2:303047/136‑1 (MQ=255)                                                                                                          ggCGTCGATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACt         >  2:996485/1‑136 (MQ=255)                                                                                                           gCGTCGATTTCGTCGATTAGGATGATGCAACGGGCGTGCATCGTGGCCAGCTCGAACATGTCGCGCACACGGCTGGCACCCACACCCCCGAACCTTTACACGAAGACCGAACCCGAAAAGGTGAAGAACGTTACtt        >  2:921056/1‑136 (MQ=255)                                                                                                                gATTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTttcgct   <  2:909004/136‑1 (MQ=255)                                                                                                                 aTTTCGTCGATGAGGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTttcgctt  >  1:829226/1‑136 (MQ=255)                                                                                                                              |                                                                                                                           GCCATCATTCATCTCGAAGCCGTCCATCTCCACCAGCAACTGGTTGAGGGTTTGCTCACGCTCGTCGTGACCGCCGCCCATGCCGGCGCCACGATGGCGACCAACGGCGTCGATTTCGTCGATGAAGATGATGCACGGGGCGTGCTTCTTGGCCTGCTCGAACATGTCGCGCACACGGCTGGCACCGACACCCACGAACATTTCCACGAAGTCCGAACCGGAAATGGTGAAGAACGGTACTTTCGCTT  >  NC_002947/5367641‑5367888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑