Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,385,507	T→C	D319G (GAC→GGC)	oprD ←	basic amino acid specific porin OprD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,507	0	T	C	100.0%	140.4 / NA	38	D319G (GAC→GGC)	oprD	basic amino acid specific porin OprD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

TTGCTGGTGCCGGCGACGTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGTCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGATGTTGTAGGCGCC  >  NC_002947/1385375‑1385634                                                                                                                                     |                                                                                                                                ttGCTGGTGCCGGCGACGTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCgg                                                                                                                              >  2:826067/1‑136 (MQ=255)       gTGCCGGCGACGTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCggg                                                                                                                        <  1:481397/136‑1 (MQ=255)                  gTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTg                                                                                                             <  2:1067054/136‑1 (MQ=255)                  gTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTg                                                                                                             <  2:778292/136‑1 (MQ=255)                      gCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCa                                                                                                         >  2:773329/1‑136 (MQ=255)                              aCCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATg                                                                                                 >  1:339936/1‑136 (MQ=255)                              aCCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATg                                                                                                 >  1:346156/1‑136 (MQ=255)                                   gTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTgcc                                                                                            >  2:381388/1‑136 (MQ=255)                                    tACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTgccg                                                                                           <  1:117895/136‑1 (MQ=255)                                     aCATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTgccgc                                                                                          >  2:681119/1‑136 (MQ=255)                                          aCGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCg                                                                                     >  1:705708/1‑136 (MQ=255)                                           cGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGt                                                                                    <  2:411176/136‑1 (MQ=255)                                           cGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTCAAACCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGt                                                                                    <  1:581976/136‑1 (MQ=255)                                              gTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGa                                                                                 <  2:49401/136‑1 (MQ=255)                                              gTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGa                                                                                 >  2:1062262/1‑136 (MQ=255)                                                 aTGAAGGGCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATac                                                                              <  1:88111/136‑1 (MQ=255)                                                    aaGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGACGATaccaa                                                                           >  1:236616/1‑135 (MQ=255)                                                               tGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAgtcg                                                                <  1:195731/136‑1 (MQ=255)                                                                    tGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAgtcgccgtc                                                           >  1:627109/1‑136 (MQ=255)                                                                    tGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGACACCATAACCATAgtcgccgtc                                                           >  1:859774/1‑136 (MQ=255)                                                                         tATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCgg                                                      >  1:826258/1‑136 (MQ=255)                                                                         tATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTAGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCgg                                                      >  2:859842/1‑136 (MQ=255)                                                                            tCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGa                                                   <  1:1068554/136‑1 (MQ=255)                                                                            tCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGa                                                   <  1:1156802/136‑1 (MQ=255)                                                                                   aGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTg                                            >  2:195050/1‑136 (MQ=255)                                                                                      gcAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTa                                         <  1:400420/135‑1 (MQ=255)                                                                                        cggTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAgg                                       <  2:1118525/135‑1 (MQ=255)                                                                                             gTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAgg                                  <  1:381388/136‑1 (MQ=255)                                                                                                  gAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGaa                             >  2:946779/1‑136 (MQ=255)                                                                                                  gAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCAAAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCAAGGGTGaa                             >  1:886564/1‑136 (MQ=255)                                                                                                                ttCTCGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCgg               >  2:619934/1‑135 (MQ=255)                                                                                                                 tcacGTCTTCGGCGTTGAAGCCGGAGCGGGCCAAGGAGTTGGACAGGAATATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGCTGAAGGTGAGAGCGCCGAt              >  2:1097569/4‑136 (MQ=255)                                                                                                                   ccGTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGAtgt            <  1:162356/135‑1 (MQ=255)                                                                                                                     gTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAgtcg                                                                <  2:28601/82‑1 (MQ=255)                                                                                                                     gTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAgtcg                                                                >  1:28601/1‑82 (MQ=255)                                                                                                                     gTCTTCGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGAtgttg          >  2:459041/1‑136 (MQ=255)                                                                                                                          cGGCGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGATGTTGTAGgc     >  1:954957/1‑136 (MQ=255)                                                                                                                             cGTTGAAGCCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGATGTTGTAGGCGcc  >  2:813258/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                                TTGCTGGTGCCGGCGACGTTGGCGTCGGAACCGCGTACATAACGGGTCATGAAGGTCAGGCCTGGAATGCCGTATTCGGCGAAGTTCAGGTCGTAGCGAGCCTGCCAGGATTTCTCGTCTTCGGCGTTGAAGTCGGAGCGGGCCACGGAGTTGGCCAGGAAGATGGTGCCGCCACCGTCGATACCATAACCATAGTCGCCGTCGCCGGTGACTTTCTGGTAGGCCAGGGTGAAGGTGTGAGCGCCGATGTTGTAGGCGCC  >  NC_002947/1385375‑1385634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑