Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,824,314 C→G A279P (GCG→CCG)  kguT ← 2‑ketogluconate transporter, putative

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,824,3140CG96.3% 93.6 / ‑3.2 27A279P (GCG→CCG) kguT2‑ketogluconate transporter, putative
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (13/13);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGA  >  NC_002947/3824189‑3824445
                                                                                                                             |                                                                                                                                   
gCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGAGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGc                                                                                                                           >  2:1080585/1‑136 (MQ=255)
       aaGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAgg                                                                                                                    <  1:5514/136‑1 (MQ=255)
                 aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc                                                                                                          <  1:1060883/136‑1 (MQ=255)
                 aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc                                                                                                          >  2:750051/1‑136 (MQ=255)
                      tAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCt                                                                                                     <  2:418827/136‑1 (MQ=255)
                        gAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGc                                                                                                   >  2:679701/1‑136 (MQ=255)
                              cAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGata                                                                                             >  1:736545/1‑136 (MQ=255)
                                 cgcgGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCg                                                                                          >  2:862578/1‑136 (MQ=255)
                                 cgcgGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCg                                                                                          >  1:745695/1‑136 (MQ=255)
                                                   cggcCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTg                                                                        >  2:713607/1‑136 (MQ=255)
                                                             aaGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACg                                                              >  2:101987/1‑136 (MQ=255)
                                                                 gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGATGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                          <  2:911200/136‑1 (MQ=255)
                                                                 gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                          <  1:126500/136‑1 (MQ=255)
                                                                 gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                          <  2:5536/136‑1 (MQ=255)
                                                                 gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg                                                          <  2:447624/136‑1 (MQ=255)
                                                                  cttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGc                                                         >  1:928870/1‑136 (MQ=255)
                                                                        cttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                   <  1:212311/136‑1 (MQ=255)
                                                                        cttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                   <  1:265610/136‑1 (MQ=255)
                                                                        cttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc                                                   <  2:939437/136‑1 (MQ=255)
                                                                             gCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGaa                                              >  1:1112520/1‑136 (MQ=255)
                                                                                ggCGGTCGGACGCCCAGGACACGCCCACCTTTGCCAGCACGGCCGGCAGGTAAGGCACGGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCGTGGTTGAGGATCGAGGGAAGCCACAGCACGAAGcc                                           <  1:517424/136‑1 (MQ=255)
                                                                                        gACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCTGGTATGGCACCGAGGCCAGCCTGCCGGCCTGCACTATATCGACGTTCGCCGCCTGCTTGTGGATCGACGGCAGCCACAGCACGAACCCATACACGc                                   <  2:452935/136‑1 (MQ=255)
                                                                                                   cacGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCcag                        <  2:72660/136‑1 (MQ=255)
                                                                                                           ccaTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACTATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTa                <  1:898940/136‑1 (MQ=255)
                                                                                                             aTTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACt              >  1:1153106/1‑136 (MQ=255)
                                                                                                              ttGCCAGCACCGCCGGCAGCTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTg             >  2:199496/1‑136 (MQ=255)
                                                                                                                   aGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAgcg        >  1:296071/1‑136 (MQ=255)
                                                                                                                         gccggcAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGga  >  2:573695/1‑136 (MQ=255)
                                                                                                                         gccggcAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCGTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGga  >  1:243773/1‑136 (MQ=255)
                                                                                                                             |                                                                                                                                   
GCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGA  >  NC_002947/3824189‑3824445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑