Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,637,297	A→G	S143P (TCT→CCT)	uvrY ←	BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,637,297	0	A	G	100.0%	149.5 / NA	40	S143P (TCT→CCT)	uvrY	BarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

GTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGC  >  NC_002947/4637182‑4637425                                                                                                                    |                                                                                                                                 gTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGtt                                                                                                              >  2:793141/1‑136 (MQ=255)      gACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAgc                                                                                                         <  1:1120294/136‑1 (MQ=255)      gACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAgc                                                                                                         <  1:323645/136‑1 (MQ=255)      gACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAgc                                                                                                         <  1:509251/136‑1 (MQ=255)         ggTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGcc                                                                                                      >  1:636661/1‑136 (MQ=255)                 gccagagGCAGAACTTGTCGGAGATGATCTGCACTTTCTGGAAGCCGACGATCATCAGGGCATTCTGGAATTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAActgctg                                                                                              <  2:694059/133‑1 (MQ=255)                 gCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGATCGGTTTCAGCGCCAActgctg                                                                                              <  2:433497/136‑1 (MQ=255)                        gCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATc                                                                                       >  2:68176/1‑136 (MQ=255)                          acaACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTg                                                                                     <  1:1002848/136‑1 (MQ=255)                           caACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGc                                                                                    <  1:546846/136‑1 (MQ=255)                            aaCTTGTGGGTGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGGCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                                   <  1:1117659/136‑1 (MQ=255)                            aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                                   <  1:1031053/136‑1 (MQ=255)                            aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                                   <  2:231914/136‑1 (MQ=255)                            aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                                   <  1:463518/136‑1 (MQ=255)                            aaCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCg                                                                                   <  1:985261/136‑1 (MQ=255)                             aCTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCgg                                                                                  >  2:973632/1‑136 (MQ=255)                                         tgatCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAgcg                                                                      >  2:857138/1‑136 (MQ=255)                                           atCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCt                                                                    <  1:188683/136‑1 (MQ=255)                                                cACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCg                                                               <  1:942242/136‑1 (MQ=255)                                                       tGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAgg                                                        <  1:282089/136‑1 (MQ=255)                                                        ggCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGc                                                       >  1:14138/1‑136 (MQ=255)                                                        ggCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGc                                                       >  2:852185/1‑136 (MQ=255)                                                        ggCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGc                                                       >  2:738822/1‑136 (MQ=255)                                                            gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                                   <  1:766600/136‑1 (MQ=255)                                                            gCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGa                                                   <  2:853906/136‑1 (MQ=255)                                                            cccGACGAGCATCAGGGCAATCTGTATTTCCCGTTCGGACAACGGGTCTAACGGAGGCCCATGCGGCTGGAACGGTTTCTGCGCCAACTGCTGGGCGTTCTGGGGGCTTATATCGCGCGGGCCGGCAAAGGCCAGa                                                   <  1:160686/135‑1 (MQ=255)                                                                acgaTCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGaa                                               >  1:988528/1‑136 (MQ=255)                                                                acgaTCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGaa                                               >  1:690725/1‑136 (MQ=255)                                                                              aaTCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCAtt                                 >  1:968506/1‑136 (MQ=255)                                                                                   ggATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTc                            <  2:561930/136‑1 (MQ=255)                                                                                   ggATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTc                            >  2:227665/1‑136 (MQ=255)                                                                                   ggATTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTc                            >  2:797115/1‑136 (MQ=255)                                                                                     aTTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCaa                          >  1:210774/1‑136 (MQ=255)                                                                                     aTTTCCCGTTCGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCaa                          >  1:507739/1‑136 (MQ=255)                                                                                               cGGACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCAcc                >  2:918400/1‑136 (MQ=255)                                                                                                ggACAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCAccc               <  2:295492/136‑1 (MQ=255)                                                                                                   cAACGCGTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTg            <  1:955780/136‑1 (MQ=255)                                                                                                     ccgcgTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGt          <  2:568960/135‑1 (MQ=255)                                                                                                     ccgcgTCGAACGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGt          <  1:201697/135‑1 (MQ=255)                                                                                                             aaCGGAGGCCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGc  <  2:60954/136‑1 (MQ=255)                                                                                                                    |                                                                                                                                 GTATTGACGGTCTTGGGCGAGAGGCACAACTTGTCGGAGATGATCTGCACTTTCTGGCAGCCGACGATCATCAGGGCAATCTGGATTTCCCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGC  >  NC_002947/4637182‑4637425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑