| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,348,779:1 | +G | 100% | intergenic (‑239/+515) | galP‑I ← / ← PP_1174 | porin‑like protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,348,779 | 1 | . | G | 80.0% | 38.5 / 5.2 | 15 | intergenic (‑239/+515) | galP‑I/PP_1174 | porin‑like protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (5/7); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCGGTAACCGGGCGCTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGT‑TGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAC > NC_002947/1348646‑1348902 | gcgcgGTAACCGGGCGCTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATgtgtgt > 2:360057/1‑134 (MQ=255) gcTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATgg > 2:100539/1‑136 (MQ=255) gcTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATgg > 2:437771/1‑136 (MQ=255) aCTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGT‑TGTCGTACAGCTATGGGTGattat > 1:502379/1‑136 (MQ=255) ggCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGT‑TGTCGTACAGCTATGGGTGATTATTAGCAAg < 2:502379/136‑1 (MQ=255) aCGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGAAAGGGGa > 2:335404/1‑136 (MQ=255) gAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGGGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGAt < 2:115117/136‑1 (MQ=255) aCAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGAt > 1:297072/1‑109 (MQ=255) aCAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGAt < 2:297072/109‑1 (MQ=255) aCATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGT‑TGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTg < 2:91623/136‑1 (MQ=255) gTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGaa > 2:505307/1‑136 (MQ=255) cTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTTATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTgg < 1:100539/136‑1 (MQ=255) cTTGTTGTTTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTgg < 1:437771/136‑1 (MQ=255) tgttTTTGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGActct < 2:305249/136‑1 (MQ=255) ttttGTTATGTGTGTGTCGTACAACTAT‑GGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAc < 1:360057/136‑1 (MQ=255) tttGTTATGTGTGTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAc < 2:320806/136‑1 (MQ=255) | GCGCGGTAACCGGGCGCTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGT‑TGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAC > NC_002947/1348646‑1348902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |