| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,748,169 | T→C | 37.5% | K270K (AAA→AAG) | etfA ← | electron transfer flavoprotein subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,748,169 | 0 | T | C | 37.5% | 11.7 / 22.8 | 24 | K270K (AAA→AAG) | etfA | electron transfer flavoprotein subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base C (6/3); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01 | |||||||||||
CAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGC > NC_002947/4748041‑4748303 | cAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCCCGATCACTTTGGAGTCTTTCATGc > 2:407941/1‑136 (MQ=255)cAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGc > 2:412680/1‑136 (MQ=255) ggCGGATCAGACCAGGTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCTGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTAGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:390423/136‑1 (MQ=255) ggCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:227756/136‑1 (MQ=255) ggCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:186486/136‑1 (MQ=255) ttCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATcgcg < 2:291647/136‑1 (MQ=255) ggAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATAc > 2:297688/1‑136 (MQ=255) tgAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt > 2:237815/2‑136 (MQ=255) cGAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt < 1:230781/136‑1 (MQ=255) accgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATcg > 1:294148/3‑104 (MQ=255) accgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATcg < 2:294148/102‑1 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:417825/1‑131 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:414346/1‑131 (MQ=255) ccGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGa > 2:180586/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 1:403618/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 2:318974/1‑136 (MQ=255) gtgcTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGcca > 2:493401/3‑134 (MQ=255) tgcTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGccag > 2:369310/2‑133 (MQ=255) cttcttCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGccagtc < 2:417825/136‑6 (MQ=255) ttcttcGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCg < 1:402828/136‑1 (MQ=255) cTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACg < 1:441569/136‑1 (MQ=255) tgATCGCCACGATCACTATGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGcc > 1:403409/1‑136 (MQ=255) cTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc < 2:329573/136‑1 (MQ=255) cTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc < 1:339183/136‑1 (MQ=255) | CAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGC > NC_002947/4748041‑4748303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |