| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,748,211 | G→A | 29.7% | A256A (GCC→GCT) | etfA ← | electron transfer flavoprotein subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,748,211 | 0 | G | A | 29.7% | 26.6 / 14.1 | 26 | A256A (GCC→GCT) | etfA | electron transfer flavoprotein subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (5/12); new base A (5/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-02 | |||||||||||
CGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGG > NC_002947/4748081‑4748343 | tgAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt > 2:237815/2‑136 (MQ=255)cGAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt < 1:230781/136‑1 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:417825/1‑131 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:414346/1‑131 (MQ=255) ccGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGa > 2:180586/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 2:318974/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 1:403618/1‑136 (MQ=255) gtgcTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGcca > 2:493401/3‑134 (MQ=255) tgcTTCTTCGTCCTTGTTGATCGCAACAATCACGCGGCTGTCTTTCATGCCCGCCAGATGCTGGATCGCACCCGAGATGCCGACTGCGATGTAAAGTTCAGGTGCAACCACCTTGCCGGTCTGGCCAACCTGccag > 2:369310/2‑133 (MQ=255) cttcttCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGCCCGTCTGGCCGACCTGccagtc < 2:417825/136‑6 (MQ=255) ttcttcGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCg < 1:402828/136‑1 (MQ=255) cTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACg < 1:441569/136‑1 (MQ=255) tgATCGCCACGATCACTATGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGcc > 1:403409/1‑136 (MQ=255) cTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc < 1:339183/136‑1 (MQ=255) cTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc < 2:329573/136‑1 (MQ=255) cATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCa > 1:301020/1‑136 (MQ=255) ttCGCGCCGTAGATACCGACGGCGATGTACAGCTGTGGCGCTACGTTCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGt < 2:301020/135‑1 (MQ=255) aTCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGt < 1:154426/136‑1 (MQ=255) aTCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGt < 1:180586/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 1:201151/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 1:297688/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGGGGCGCAACGAACTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 2:360814/136‑1 (MQ=255) gcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGcc < 1:412680/136‑1 (MQ=255) gcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGcc < 1:407941/136‑1 (MQ=255) cGGAGATACCGACGGCGAAGTACAGCTCTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCaa > 1:461135/1‑135 (MQ=255) gACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGACGGCAAGGCTGTACAgg > 2:310422/1‑136 (MQ=255) | CGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGG > NC_002947/4748081‑4748343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |