| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,827,483 | A→G | 21.0% | Y1892H (TAC→CAC) | pvdL ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,827,483 | 0 | A | G | 21.0% | 31.9 / 5.6 | 19 | Y1892H (TAC→CAC) | pvdL | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (5/10); new base G (2/2); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GACTGCCGGACGCGGGCGGTCGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTGACGATGTGATGCA > NC_002947/4827355‑4827608 | gACTGCCGCACGCGGGCGGTCGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAgg < 1:52482/136‑1 (MQ=255) gACGCGGGCGGTCGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCAc > 1:506208/1‑136 (MQ=255) gggcggTCGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTTGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCa > 2:245684/1‑136 (MQ=255) gggcGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAgcgg < 1:318927/133‑1 (MQ=255) cAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCa < 2:314086/136‑1 (MQ=255) cAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCa < 2:181119/136‑1 (MQ=255) tAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGtg > 1:282087/1‑135 (MQ=255) ttGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCg < 1:339081/136‑1 (MQ=255) gTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGat < 1:291493/136‑2 (MQ=255) gTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGaa < 1:17197/136‑1 (MQ=255) gTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGaa > 2:494616/1‑136 (MQ=255) ccTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCAt < 2:333508/136‑1 (MQ=255) gccgcTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGcc < 2:402722/136‑1 (MQ=255) ccATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTg < 1:494616/136‑1 (MQ=255) gTTGCCACACGCTGTGGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTg < 1:293006/128‑1 (MQ=255) gTTGCCACACGCTGTGGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTg < 1:290438/128‑1 (MQ=255) gTTGCCACACGCTGTGGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTg > 2:290438/1‑128 (MQ=255) gTTGCCACACGCTGTGGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTg > 2:293006/1‑128 (MQ=255) cacaCGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTGACGATGTGATGCa > 2:177508/1‑136 (MQ=255) | GACTGCCGGACGCGGGCGGTCGGCAGGCAACGCCAGCACCGGGTGCTCGTCGCCCAGGCGGTGCTTCCAGTAGGCCAGTTGTCGAGCGCCCTCGCCGCTTTCCAGCCATTGCCGTTGCCACACGCTGTAGTCCAGGTACTGCACCGGCAGCGGCGCCAGTGGCGACTCGCGGTCATCGACAAAGGCTTCGTACAGCTCGCCCAGTTCGCGGGCGAAGATGTCCATGGCCCAGCCTTCGGTGACGATGTGATGCA > NC_002947/4827355‑4827608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |