| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,872,114 | G→T | 20.0% | V633L (GTG→TTG) ‡ | xdhB → | xanthine dehydrogenase subunit XdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,872,114 | 0 | G | T | 20.0% | 43.5 / 6.6 | 20 | V633L (GTG→TTG) ‡ | xdhB | xanthine dehydrogenase subunit XdhB |
| Reads supporting (aligned to +/- strand): ref base G (5/11); new base T (2/2); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCAT > NC_002947/4871991‑4872235 | cagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGAtc‑g < 2:169304/136‑1 (MQ=255) aCTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCCGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATAc > 1:210750/1‑136 (MQ=255) ccAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGAcc < 2:292474/136‑1 (MQ=255) aGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCgg > 1:166116/1‑136 (MQ=255) tCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGc < 1:249646/124‑1 (MQ=255) tCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGc > 2:249646/1‑124 (MQ=255) cAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATg < 2:424773/136‑1 (MQ=255) gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 2:34102/136‑1 (MQ=255) gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 1:399389/136‑1 (MQ=255) cACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTg > 2:53081/1‑136 (MQ=255) gggTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTgcgcg < 2:78990/136‑1 (MQ=255) gCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCt < 1:114681/136‑1 (MQ=255) aTCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTgg < 1:468863/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGTCCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGTCGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:161737/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGAGATC‑GTCGATACGCTGACCGGCGAATACACGATGTTGCGCGCCGACATCCTGCACGACGGGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:130252/136‑1 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc > 1:392411/4‑136 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc < 1:104055/133‑1 (MQ=255) cTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGTTGGTTTc > 2:52571/1‑136 (MQ=255) tatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcg < 1:400930/134‑2 (MQ=255) atGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcgt > 2:511496/2‑134 (MQ=255) | CAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCAT > NC_002947/4871991‑4872235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |