| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,872,149 | C→T | 25.0% | Y644Y (TAC→TAT) | xdhB → | xanthine dehydrogenase subunit XdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,872,149 | 0 | C | T | 25.0% | 23.3 / 5.2 | 16 | Y644Y (TAC→TAT) | xdhB | xanthine dehydrogenase subunit XdhB |
| Reads supporting (aligned to +/- strand): ref base C (4/8); new base T (2/2); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
CAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCAAA > NC_002947/4872020‑4872284 | cAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATg < 2:424773/136‑1 (MQ=255) gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 2:34102/136‑1 (MQ=255) gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 1:399389/136‑1 (MQ=255) cACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTg > 2:53081/1‑136 (MQ=255) gggTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTgcgcg < 2:78990/136‑1 (MQ=255) gCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCt < 1:114681/136‑1 (MQ=255) aTCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTgg < 1:468863/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGTCCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGTCGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:161737/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGAGATC‑GTCGATACGCTGACCGGCGAATACACGATGTTGCGCGCCGACATCCTGCACGACGGGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:130252/136‑1 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc > 1:392411/4‑136 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc < 1:104055/133‑1 (MQ=255) cTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGTTGGTTTc > 2:52571/1‑136 (MQ=255) tatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcg < 1:400930/134‑2 (MQ=255) atGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcgt > 2:511496/2‑134 (MQ=255) cgATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGaa > 1:359972/1‑136 (MQ=255) tACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAagag > 1:208449/1‑136 (MQ=255) cAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCaaa > 2:506664/1‑136 (MQ=255) | CAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCAAA > NC_002947/4872020‑4872284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |