| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,872,157 | T→A | 26.7% | L647* (TTG→TAG) ‡ | xdhB → | xanthine dehydrogenase subunit XdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,872,157 | 0 | T | A | 26.7% | 19.9 / 5.7 | 15 | L647* (TTG→TAG) ‡ | xdhB | xanthine dehydrogenase subunit XdhB |
| Reads supporting (aligned to +/- strand): ref base T (6/5); new base A (2/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
GCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCAAAGGCAAG > NC_002947/4872022‑4872290 | gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 2:34102/136‑1 (MQ=255)gCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGtt < 1:399389/136‑1 (MQ=255) cACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTg > 2:53081/1‑136 (MQ=255) gggTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTgcgcg < 2:78990/136‑1 (MQ=255) gCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCt < 1:114681/136‑1 (MQ=255) aTCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTgg < 1:468863/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGTCCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGTCGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:161737/136‑1 (MQ=255) gTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGAGATC‑GTCGATACGCTGACCGGCGAATACACGATGTTGCGCGCCGACATCCTGCACGACGGGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAg < 1:130252/136‑1 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc > 1:392411/4‑136 (MQ=255) ctatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTc < 1:104055/133‑1 (MQ=255) cTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGTTGGTTTc > 2:52571/1‑136 (MQ=255) tatGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcg < 1:400930/134‑2 (MQ=255) atGGCGCGGCCTGCTCGGAAGT‑ATCGGTCGATACGCTGACCGGCGAATATGTGGTGGAGCGCACCGATATTCTTCACGATACGGGCCGTTCGCTGAACCGTGTGATTGATATTGGTCAGGTGGAAGGCGGCTTcgt > 2:511496/2‑134 (MQ=255) cgATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGaa > 1:359972/1‑136 (MQ=255) tACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAagag > 1:208449/1‑136 (MQ=255) cAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCaaa > 2:506664/1‑136 (MQ=255) gTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCAAAGGCAAg > 1:387105/1‑136 (MQ=255) | GCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATC‑GTCGATACGCTGACCGGCGAATACAAGATGTTGCGCGCCGACATCCTGCACGACGTGGGCGATTCGCTGAACCCTGCGATCGACATCGGCCAGGTGGAGGGTGGTTTCATCCAGGGCATGGGCTGGCTGACCACCGAAGAGCTGGTGTGGAACGCCAAAGGCAAG > NC_002947/4872022‑4872290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |