| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,359,049 | C→T | 25.0% | G561D (GGC→GAC) | infB ← | translation initiation factor IF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,359,049 | 0 | C | T | 25.0% | 23.8 / 4.4 | 16 | G561D (GGC→GAC) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base C (4/8); new base T (2/2); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAACCACGGACAGCTCGTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCGGGCCACGGCCCTTGTCCAGGCGCGATTCGACGACCACACCACGACC > NC_002947/5358924‑5359167 | cgGCAACCACGGACAGCTCGTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGa > 1:283300/1‑136 (MQ=255) aaCCACGGACAGCTCGTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCg > 2:261502/1‑136 (MQ=255) aCGGACAGCTCGTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGcaca < 1:462494/136‑1 (MQ=255) cGTCACCGGCTTCCGGAGTACCGTCCAGGCCGTGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTc < 2:403097/136‑1 (MQ=255) gTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTg < 1:349609/115‑1 (MQ=255) gTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTg > 2:349609/1‑115 (MQ=255) gCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGAc < 2:246404/136‑1 (MQ=255) aCCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCg < 1:261502/136‑1 (MQ=255) aGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGTCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGaa > 2:425457/1‑136 (MQ=255) aGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGTCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGaa > 2:427846/1‑136 (MQ=255) cGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGTCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTcgc < 1:425457/136‑1 (MQ=255) cGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGTCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTcgc < 1:427846/136‑1 (MQ=255) gATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCggg < 1:146062/136‑1 (MQ=255) tCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCGGGcc > 1:267900/1‑136 (MQ=255) cGGGCCAGCTTCCTTCACAGTCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCGGGCCACgg < 2:312749/136‑1 (MQ=255) ccagggCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCGGGCCACGGCCCTTGTCCAGGCGCGATTCGACGACCACACCACGAcc < 1:325318/132‑1 (MQ=255) | CGGCAACCACGGACAGCTCGTCACCGGCTTCCGGAGTACCGTCCAGGCCGAGGATTTCGACCGGGATCGACGGGCCAGCTTCCTTCACAGGCTTGCCGTTCTCGTCGAGCATGGCGCGAACGCGGCCATAGTTGGAACCGCACAGGACCATGTCGCCCTGACGCAGGGTACCGTCCTGAACCAGGATGGTCGCGACCGGGCCACGGCCCTTGTCCAGGCGCGATTCGACGACCACACCACGACC > NC_002947/5358924‑5359167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |