| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,378,335 | A→G | 22.2% | G400G (GGT→GGC) | dnaK ← | chaperone protein DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,378,335 | 0 | A | G | 22.2% | 28.7 / 5.9 | 18 | G400G (GGT→GGC) | dnaK | chaperone protein DnaK |
| Reads supporting (aligned to +/- strand): ref base A (8/6); new base G (2/2); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
ACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTTACCGAAGAA > NC_002947/5378200‑5378469 | aCGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATa < 1:325427/136‑1 (MQ=255) cGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATAc > 2:353942/1‑136 (MQ=255) gTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTg < 1:353942/136‑1 (MQ=255) aaTGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACg > 1:11370/1‑136 (MQ=255) ggTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTc < 2:254107/136‑1 (MQ=255) gtcgGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCACGGTCAGCGGGTTGACGTCCAGCAGCACGACGTCTTTCACg > 2:203521/1‑136 (MQ=255) cggTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTc > 1:112988/3‑133 (MQ=255) cggTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTc > 1:110558/3‑133 (MQ=255) cggTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTc < 2:110558/131‑1 (MQ=255) cggTCGAGAAGGTCTGCGACTTCTTGGTCGGGATCGTCGTGTTGCGTTCGATCAGGCGGGTGAACACGCCACCGAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGAACGTCCTTGACGTc < 2:112988/131‑1 (MQ=255) cAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCAcc < 2:271845/136‑1 (MQ=255) gTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCgg < 2:335675/136‑1 (MQ=255) gTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCgg < 2:127606/136‑1 (MQ=255) cGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCAccc > 1:400764/1‑136 (MQ=255) tCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGTTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGgcagc < 1:203521/136‑1 (MQ=255) ttctCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCtt > 1:319177/1‑136 (MQ=255) cgGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGAc > 2:510256/1‑136 (MQ=255) tACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTTACCgaagaa > 1:385671/1‑136 (MQ=255) tACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTTACCgaagaa > 1:27966/1‑136 (MQ=255) | ACGCTCACCTTGCAGCACGTGAATGGTCACGGCGCTCTGGTTGTCGTCGGCAGTCGAGAACACCTGCGACTTCTTGGTCGGGATGGTGGTGTTCTTCTCGATCAGCGCGGTCATCACGCCGCCCATGGTTTCGATACCCAGGGTCAGCGGGCTGACGTCCAGCAGCAGGACGTCTTTCACGTCACCGGCCAGTACCGCACCCTGGATGGCAGCACCCATGGCAACGGCTTCGTCCGGGTTGACGTCCTTGCGCGCTTCTTTACCGAAGAA > NC_002947/5378200‑5378469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |