| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,605,111 | T→A | 30.8% | E115V (GAA→GTA) | PP_4924 ← | subtilase family serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,605,111 | 0 | T | A | 30.8% | 11.5 / 5.9 | 13 | E115V (GAA→GTA) | PP_4924 | subtilase family serine protease |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base A (2/2); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
TGTAAATTCATCCGTTAGATTCCCCGCTGAGGAAAGCACATGCCGCCAGTAGTCCTCTCGATTTTCGACAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCAACCCTCACC > NC_002947/5604984‑5605230 | tgtAAATTCATCCGTTAGATTCCCCGCTGAGGAAAGCACATGCCGCCAGTAGTCCTCTCGATTTTCGACAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGtt > 2:299023/1‑136 (MQ=255) gccAGTAGTCCTCTCGATTTTCGACAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTTGCTTACCTGACCGAc < 1:299023/136‑1 (MQ=255) tagtCCTCTCGATTTTCGACAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACAGACAATAg > 2:481160/1‑136 (MQ=255) aCAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTACGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAAtcttct > 1:298461/1‑136 (MQ=255) aCAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTACGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAAtcttct > 1:300329/1‑136 (MQ=255) tCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTACGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCaa < 2:298461/136‑1 (MQ=255) tCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTACGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCaa < 2:300329/136‑1 (MQ=255) cTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAg < 1:305642/94‑1 (MQ=255) cTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAg > 2:305642/1‑94 (MQ=255) ccTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCAAcc < 2:426057/136‑1 (MQ=255) aGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCaa > 1:425899/1‑128 (MQ=255) aGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCaa < 2:425899/128‑1 (MQ=255) aGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCAACCCTCAcc > 1:57401/1‑136 (MQ=255) | TGTAAATTCATCCGTTAGATTCCCCGCTGAGGAAAGCACATGCCGCCAGTAGTCCTCTCGATTTTCGACAGCAATCACATCAAACACCGGACTCAACGTCCATGCCCTCTCAGGATATTCAAGGCTTTCGAAAGTTTCCTTATGAAATGCCGCGACCTCTTTTTGGCTTACCTGACCGACAATAGGACCTTTACTAATCTTCTTTAGCAACACCTCCATATATTTATTTGCCATTTCAACCCTCACC > NC_002947/5604984‑5605230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |