| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,851,574 | G→T | 20.0% | A101S (GCC→TCC) | ilvD → | dihydroxy‑acid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,851,574 | 0 | G | T | 20.0% | 48.6 / 6.6 | 20 | A101S (GCC→TCC) | ilvD | dihydroxy‑acid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base T (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC > NC_002947/5851443‑5851704 | gCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCgc > 1:258537/1‑135 (MQ=255) cAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACg < 2:457227/136‑1 (MQ=255) cTGGTGGCTCGCTAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCg < 2:186118/136‑1 (MQ=255) cTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCg < 2:359970/136‑1 (MQ=255) ggCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGaa < 1:171420/136‑1 (MQ=255) cGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAAt < 2:50172/136‑1 (MQ=255) ccAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCCCTGCGCCGACGCCAt > 1:508664/1‑136 (MQ=255) cgGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGa > 1:47309/1‑136 (MQ=255) aTGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGAt < 1:115996/136‑1 (MQ=255) ccATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCAt < 2:456282/136‑1 (MQ=255) gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg > 1:294232/1‑84 (MQ=255) gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg < 2:294232/84‑1 (MQ=255) catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg > 2:183251/4‑136 (MQ=255) catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg > 2:385779/4‑136 (MQ=255) cgGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGgccgcc > 1:13368/1‑136 (MQ=255) ggCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGccc > 2:199613/1‑136 (MQ=255) gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc > 1:111177/1‑136 (MQ=255) gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc > 1:113604/1‑136 (MQ=255) gCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGAt > 2:363677/1‑136 (MQ=255) tgaTTTCCGACTCGGTTGAATATATAGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATGGCAGCACTCCGCCTCAATATCCCGGTCGTGTTCGTTTc < 1:286242/134‑1 (MQ=255) | GCCAGCTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC > NC_002947/5851443‑5851704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |