Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,851,582	C→G	22.2%	A103A (GCC→GCG) ‡	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,851,582	0	C	G	22.2%	36.4 / 7.4	18	A103A (GCC→GCG) ‡	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (8/6);  new base G (2/2);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC  >  NC_002947/5851448‑5851704                                                                                                                                       |                                                                                                                           cTGGTGGCTCGCTAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCg                                                                                                                           <  2:186118/136‑1 (MQ=255) cTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCg                                                                                                                           <  2:359970/136‑1 (MQ=255)      ggCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGaa                                                                                                                      <  1:171420/136‑1 (MQ=255)                  cGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAAt                                                                                                          <  2:50172/136‑1 (MQ=255)                                      ccAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCCCTGCGCCGACGCCAt                                                                                      >  1:508664/1‑136 (MQ=255)                                                           cgGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGa                                                                 >  1:47309/1‑136 (MQ=255)                                                                 aTGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGAt                                                           <  1:115996/136‑1 (MQ=255)                                                                             ccATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCAt                                               <  2:456282/136‑1 (MQ=255)                                                                                   gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg                                                                                             >  1:294232/1‑84 (MQ=255)                                                                                   gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg                                                                                             <  2:294232/84‑1 (MQ=255)                                                                                     catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg                                       >  2:183251/4‑136 (MQ=255)                                                                                     catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg                                       >  2:385779/4‑136 (MQ=255)                                                                                          cgGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGgccgcc                                  >  1:13368/1‑136 (MQ=255)                                                                                           ggCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGccc                                 >  2:199613/1‑136 (MQ=255)                                                                                                         gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc                   >  1:111177/1‑136 (MQ=255)                                                                                                         gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc                   >  1:113604/1‑136 (MQ=255)                                                                                                                 gCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGAt           >  2:363677/1‑136 (MQ=255)                                                                                                                          tgaTTTCCGACTCGGTTGAATATATAGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATGGCAGCACTCCGCCTCAATATCCCGGTCGTGTTCGTTTc  <  1:286242/134‑1 (MQ=255)                                                                                                                                       |                                                                                                                           CTGGTGGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC  >  NC_002947/5851448‑5851704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑