| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,851,585 | G→T | 25.0% | V104V (GTG→GTT) | ilvD → | dihydroxy‑acid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,851,585 | 0 | G | T | 25.0% | 28.2 / 6.8 | 16 | V104V (GTG→GTT) | ilvD | dihydroxy‑acid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (8/4); new base T (2/2); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC > NC_002947/5851453‑5851704 | ggCGCGCGAAATTGAAGCAGCTGGCGGCGTTGCCAAGGAATTCAACACCATTGCGGTCGATGACGGTATCGCGATGGGCCATGACGGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGaa < 1:171420/136‑1 (MQ=255) cGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAAt < 2:50172/136‑1 (MQ=255) ccAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCCCTGCGCCGACGCCAt > 1:508664/1‑136 (MQ=255) cgGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGa > 1:47309/1‑136 (MQ=255) aTGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGAt < 1:115996/136‑1 (MQ=255) ccATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCAt < 2:456282/136‑1 (MQ=255) gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg > 1:294232/1‑84 (MQ=255) gCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCg < 2:294232/84‑1 (MQ=255) catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg > 2:183251/4‑136 (MQ=255) catgacgGCATGCTCTATTCGCTGCCATCGCGCGAGCTGATTTCCGACTCGGTTGAATATATGGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATgg > 2:385779/4‑136 (MQ=255) cgGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGgccgcc > 1:13368/1‑136 (MQ=255) ggCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGccc > 2:199613/1‑136 (MQ=255) gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc > 1:111177/1‑136 (MQ=255) gCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATc > 1:113604/1‑136 (MQ=255) gCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGAt > 2:363677/1‑136 (MQ=255) tgaTTTCCGACTCGGTTGAATATATAGTCAATGCCCATTGCGCCGACGCGATGGTCTGCATTTCCAACTGCGACAAGATCACTCCCGGCATGCTGATGGCAGCACTCCGCCTCAATATCCCGGTCGTGTTCGTTTc < 1:286242/134‑1 (MQ=255) | GGCTCGCGAAATCGAACGCGCCGGTGGCGTGGCCAAGGAATTCAACACCATCGCGGTCGATGACGGCATCGCCATGGGCCACGACGGCATGCTGTACTCGCTGCCAAGCCGCGAAATCATTGCCGACGCCGTGGAATACATGGTCAATGCGCACTGCGCCGACGCCATCGTCTGTATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCCCTGCGCCTGAACATCCCGGTGATCTTCGTTTC > NC_002947/5851453‑5851704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |